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- W2154373252 abstract "epithelial cell ratio of>5,76.6% (220of287)ofrespiratory specimens received inthelaboratory wereconsidered acceptable. Unacceptable specimens weremorecommoninyounger patients. Thepositive predictive value oftheGramstain forgrowth fromacceptable sputumsamples was98%forPseudomonas aeruginosa, 84.4%forPseudomonas cepacia, 86.3%forStaphylococcus aureus, and100%forHaemophilus influenzae. Incystic fibrosis patients, ashasbeen reported forrespiratory specimens ingeneral, Gramstain ofrespiratory specimens ishelpful forinterpreting culture results. Gramstain ofsputumisestablished asanimportant component ofthebacteriological investigation oflower respiratory tract infection (2,14). Withmicroscopy atlower power(x100), thedegree ofsalivary contamination andthus thesuitability ofthespecimen forculture canbeassessed, andathigher powerunder oilimmersion (x1,000), presumptive identification ofpathogenic bacteria canbemade.From thelaboratory standpoint, examination ofastained sputum smearcanbeoffurther valueinenhancing thequality control ofculture mediausedfortheprimary isolation of specific bacteria. Cystic fibrosis (CF)isthemostcommonlethal genetic disorder inCaucasians (6). Progressive pulmonary disease commonly associated withpulmonary infection istheprincipal cause ofmorbidity andmortality inCF(18). Yet,there islittle published information onthevalue oftheGramstain inthebacteriological workupofsputumsubmitted from patients withthisdisorder. Itspossible roleinassessing sample quality orinpresumptively identifying themajor CF respiratory pathogens (Staphylococcus aureus, Haemophilus influenzae, Pseudomonas aeruginosa, and,inmany medical centers, Pseudomonas cepacia) hasyettobedefined. Thepurpose ofthepresent study wastherefore toevaluate theutility oftheGramstain inassessing thedegree of salivary contamination ofCFrespiratory specimens andin predicting thepresence ofspecific pathogens inculture on thebasis ofstaining andcellular morphological characteristics." @default.
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- W2154373252 date "1994-01-01" @default.
- W2154373252 modified "2023-09-27" @default.
- W2154373252 title "Utility ofGramStain inEvaluation ofSputafromPatients withCystic Fibrosis" @default.
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