FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2154422221> ?p ?o ?g. }

• W2154422221 endingPage "649" @default.
• W2154422221 startingPage "641" @default.
• W2154422221 abstract "(California poppy) is an emerging model plant for ‘evo–devo’ studies from the basal eudicot clade of Papaveraceae. California poppy has a relatively small genome, a short life cycle and, most importantly, it is amenable for transformation. However, since this transformation protocol is time consuming, virus-induced gene silencing (VIGS) was evaluated as a fast method to obtain functional data for California poppy genes. Commercially available California poppy plants were infiltrated with Agrobacterium tumefaciens carrying the tobacco rattle virus plasmids pTRV1 and pTRV2. pTRV2 contained part of the eschscholzia Phytoene Desaturase (EcPDS) gene whose loss of function results in photobleaching of the green parts of the plant and in a lack of floral coloration. The degree and duration of these symptoms was evaluated for vegetative rosettes and plants in flower. It is shown that VIGS is able to effectively down-regulate the EcPDS gene in eschscholzia. Various degrees of silencing were observed starting <2 weeks after infiltration with Agrobacterium tumefaciens in 92 % of the plants. Tissue with silencing symptoms also showed complete or strong reduction of EcPDS transcripts. Strong silencing resulted in almost completely white petals, fruits, shoots and leaves. Plants with a strong degree of silencing will eventually die off; however, others are able to produce EcPDS gene product even after a strong initial silencing and will recover. Silencing was found to be not always systemic, but was often restricted to certain organs or parts of organs. VIGS is an effective, fast and transient method to down-regulate gene expression in eschscholzia. It serves well to detect prominent phenotypes which may become obvious even if some target gene transcript remains in the plant tissue. However, subtle phenotypes will be more difficult to detect, as extremely strong silencing effects occur in <10 % of all flowers from infected plants." @default.
• W2154422221 created "2016-06-24" @default.
• W2154422221 creator A5033099839 @default.
• W2154422221 creator A5083044777 @default.
• W2154422221 creator A5086495285 @default.
• W2154422221 creator A5087608045 @default.
• W2154422221 date "2007-07-06" @default.
• W2154422221 modified "2023-10-01" @default.
• W2154422221 title "Highly Efficient Virus-induced Gene Silencing (VIGS) in California Poppy (Eschscholzia californica): An Evaluation of VIGS as a Strategy to Obtain Functional Data from Non-model Plants" @default.
• W2154422221 cites W1237637177 @default.
• W2154422221 cites W1480262691 @default.
• W2154422221 cites W1489746883 @default.
• W2154422221 cites W1598933278 @default.
• W2154422221 cites W1973731551 @default.
• W2154422221 cites W1981625866 @default.
• W2154422221 cites W2008803099 @default.
• W2154422221 cites W2011612912 @default.
• W2154422221 cites W2022187444 @default.
• W2154422221 cites W2028747894 @default.
• W2154422221 cites W2057225872 @default.
• W2154422221 cites W2102825463 @default.
• W2154422221 cites W2106236530 @default.
• W2154422221 cites W2115466911 @default.
• W2154422221 cites W2120824464 @default.
• W2154422221 cites W2121633927 @default.
• W2154422221 cites W2127373960 @default.
• W2154422221 cites W2132350017 @default.
• W2154422221 cites W2136643521 @default.
• W2154422221 cites W2139564786 @default.
• W2154422221 cites W2146112286 @default.
• W2154422221 cites W2147849220 @default.
• W2154422221 cites W2148218336 @default.
• W2154422221 cites W2148257122 @default.
• W2154422221 cites W2156097553 @default.
• W2154422221 cites W2160702064 @default.
• W2154422221 cites W2172102397 @default.
• W2154422221 cites W2173376188 @default.
• W2154422221 cites W2980373194 @default.
• W2154422221 doi "https://doi.org/10.1093/aob/mcm118" @default.
• W2154422221 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2533598" @default.
• W2154422221 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17616562" @default.
• W2154422221 hasPublicationYear "2007" @default.
• W2154422221 type Work @default.
• W2154422221 sameAs 2154422221 @default.
• W2154422221 citedByCount "113" @default.
• W2154422221 countsByYear W21544222212012 @default.
• W2154422221 countsByYear W21544222212013 @default.
• W2154422221 countsByYear W21544222212014 @default.
• W2154422221 countsByYear W21544222212015 @default.
• W2154422221 countsByYear W21544222212016 @default.
• W2154422221 countsByYear W21544222212017 @default.
• W2154422221 countsByYear W21544222212018 @default.
• W2154422221 countsByYear W21544222212019 @default.
• W2154422221 countsByYear W21544222212020 @default.
• W2154422221 countsByYear W21544222212021 @default.
• W2154422221 countsByYear W21544222212022 @default.
• W2154422221 countsByYear W21544222212023 @default.
• W2154422221 crossrefType "journal-article" @default.
• W2154422221 hasAuthorship W2154422221A5033099839 @default.
• W2154422221 hasAuthorship W2154422221A5083044777 @default.
• W2154422221 hasAuthorship W2154422221A5086495285 @default.
• W2154422221 hasAuthorship W2154422221A5087608045 @default.
• W2154422221 hasBestOaLocation W21544222211 @default.
• W2154422221 hasConcept C104317684 @default.
• W2154422221 hasConcept C119056186 @default.
• W2154422221 hasConcept C204241405 @default.
• W2154422221 hasConcept C2776162087 @default.
• W2154422221 hasConcept C2776295790 @default.
• W2154422221 hasConcept C2780971368 @default.
• W2154422221 hasConcept C2908719506 @default.
• W2154422221 hasConcept C54355233 @default.
• W2154422221 hasConcept C59822182 @default.
• W2154422221 hasConcept C86803240 @default.
• W2154422221 hasConceptScore W2154422221C104317684 @default.
• W2154422221 hasConceptScore W2154422221C119056186 @default.
• W2154422221 hasConceptScore W2154422221C204241405 @default.
• W2154422221 hasConceptScore W2154422221C2776162087 @default.
• W2154422221 hasConceptScore W2154422221C2776295790 @default.
• W2154422221 hasConceptScore W2154422221C2780971368 @default.
• W2154422221 hasConceptScore W2154422221C2908719506 @default.
• W2154422221 hasConceptScore W2154422221C54355233 @default.
• W2154422221 hasConceptScore W2154422221C59822182 @default.
• W2154422221 hasConceptScore W2154422221C86803240 @default.
• W2154422221 hasIssue "3" @default.
• W2154422221 hasLocation W21544222211 @default.
• W2154422221 hasLocation W21544222212 @default.
• W2154422221 hasLocation W21544222213 @default.
• W2154422221 hasLocation W21544222214 @default.
• W2154422221 hasOpenAccess W2154422221 @default.
• W2154422221 hasPrimaryLocation W21544222211 @default.
• W2154422221 hasRelatedWork W159188555 @default.
• W2154422221 hasRelatedWork W2098257518 @default.
• W2154422221 hasRelatedWork W2154422221 @default.
• W2154422221 hasRelatedWork W2311455528 @default.
• W2154422221 hasRelatedWork W2313253475 @default.
• W2154422221 hasRelatedWork W245583574 @default.
• W2154422221 hasRelatedWork W2810255408 @default.
• W2154422221 hasRelatedWork W3126178684 @default.

• next