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• W2154428305 endingPage "3419" @default.
• W2154428305 startingPage "3413" @default.
• W2154428305 abstract "Vinyl chloride (VC) is a toxic groundwater pollutant associated with plastic manufacture and chlorinated solvent use. Aerobic bacteria that grow on VC as a carbon and energy source can evolve in the laboratory from bacteria that grow on ethene, but the genetic changes involved are unknown. We investigated VC adaptation in two variants (JS623-E and JS623-T) of the ethene-oxidizing Mycobacterium strain JS623. Missense mutations in the EtnE gene developed at two positions (W243 and R257) in cultures exposed to VC but not in cultures maintained on ethene. Epoxyalkane-coenzyme M transferase (EaCoMT) activities in cell extracts of JS623-E and JS623-T (150 and 645 nmol/min/mg protein, respectively) were higher than that of wild-type JS623 (74 nmol/min/mg protein), and in both variant cultures epoxyethane no longer accumulated during growth on ethene. The heterologous expression of two variant etnE alleles (W243G [etnE1] and R257L [etnE2]) from strain JS623 in Mycobacterium smegmatis showed that they had 42 to 59% higher activities than the wild type. Recombinant JS623 cultures containing mutant EtnE genes cloned in the vector pMV261 adapted to growth on VC more rapidly than the wild-type JS623 strain, with incubation times of 60 days (wild type), 1 day (pMVetnE1), and 35 days (pMVetnE2). The JS623(pMVetnE) culture did not adapt to VC after more than 60 days of incubation. Adaptation to VC in strain JS623 is consistently associated with two particular missense mutations in the etnE gene that lead to higher EaCoMT activity. This is the first report to pinpoint a genetic change associated with the transition from cometabolic to growth-linked VC oxidation in bacteria." @default.
• W2154428305 created "2016-06-24" @default.
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• W2154428305 creator A5030495537 @default.
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• W2154428305 date "2010-06-01" @default.
• W2154428305 modified "2023-10-06" @default.
• W2154428305 title "Association of Missense Mutations in Epoxyalkane Coenzyme M Transferase with Adaptation of <i>Mycobacterium</i> sp. Strain JS623 to Growth on Vinyl Chloride" @default.
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• W2154428305 doi "https://doi.org/10.1128/aem.01320-09" @default.
• W2154428305 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2876445" @default.
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• W2154428305 hasPublicationYear "2010" @default.
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