FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2154633231> ?p ?o ?g. }

• W2154633231 endingPage "26388" @default.
• W2154633231 startingPage "26383" @default.
• W2154633231 abstract "Accumulating evidence indicates that calcification by isolated mammalian matrix vesicles (MVs) can be initiated by ATP. Since ATP can be hydrolyzed by either a specific ATPase or by nonspecific alkaline phosphatase (ALP), it remains to be established whether ATPase or ALP mediates ATP-initiated Ca and Pi deposition. To support the hypothesis that specific ATPase is responsible for ATP-initiated calcification by MVs isolated from mammalian cartilage and bone, the effects of ATP analogs, ALP substrates, and specific inhibitors on ATP hydrolysis and ATP-initiated calcification were compared between intact MVs and monoclonal antibody affinity-purified MV ALP. ATP analogs such as ADP and AMP exerted marked inhibitory effects on both [γ-32P]ATP hydrolysis and ATP-initiated calcification by intact MVs, whereas phosphomonoesters such as β-glycerophosphate or phosphoethanolamine had no effect. In contrast to intact MVs, purified MV ALP failed to calcify, and its [γ-32P]ATP hydrolytic activity was readily inhibited by phosphomonoesters. Additionally, [γ-32P]ATP hydrolysis by purified ALP in contrast to that by intact vesicles was completely inhibited by l-tetramisole, a specific inhibitor of ALP, suggesting a loss of specific ATPase during purification. Vanadate inhibition of ATP hydrolysis by purified ALP can be decreased by increasing ATP concentrations. On the contrary, ATP concentrations did not affect vanadate inhibition of ATP hydrolysis by intact MVs if ALP activity was blocked by l-tetramisole. These observations, therefore, suggest that: 1) a portion of [γ-32P]ATP hydrolysis by MVs is attributable to a specific ATPase, whereas the remaining activity is due to ALP; and 2) a specific ATPase, but not ALP, is responsible for ATP-dependent Ca- and Pi-depositing activity of MVs isolated from bone or cartilage. Accumulating evidence indicates that calcification by isolated mammalian matrix vesicles (MVs) can be initiated by ATP. Since ATP can be hydrolyzed by either a specific ATPase or by nonspecific alkaline phosphatase (ALP), it remains to be established whether ATPase or ALP mediates ATP-initiated Ca and Pi deposition. To support the hypothesis that specific ATPase is responsible for ATP-initiated calcification by MVs isolated from mammalian cartilage and bone, the effects of ATP analogs, ALP substrates, and specific inhibitors on ATP hydrolysis and ATP-initiated calcification were compared between intact MVs and monoclonal antibody affinity-purified MV ALP. ATP analogs such as ADP and AMP exerted marked inhibitory effects on both [γ-32P]ATP hydrolysis and ATP-initiated calcification by intact MVs, whereas phosphomonoesters such as β-glycerophosphate or phosphoethanolamine had no effect. In contrast to intact MVs, purified MV ALP failed to calcify, and its [γ-32P]ATP hydrolytic activity was readily inhibited by phosphomonoesters. Additionally, [γ-32P]ATP hydrolysis by purified ALP in contrast to that by intact vesicles was completely inhibited by l-tetramisole, a specific inhibitor of ALP, suggesting a loss of specific ATPase during purification. Vanadate inhibition of ATP hydrolysis by purified ALP can be decreased by increasing ATP concentrations. On the contrary, ATP concentrations did not affect vanadate inhibition of ATP hydrolysis by intact MVs if ALP activity was blocked by l-tetramisole. These observations, therefore, suggest that: 1) a portion of [γ-32P]ATP hydrolysis by MVs is attributable to a specific ATPase, whereas the remaining activity is due to ALP; and 2) a specific ATPase, but not ALP, is responsible for ATP-dependent Ca- and Pi-depositing activity of MVs isolated from bone or cartilage." @default.
• W2154633231 created "2016-06-24" @default.
• W2154633231 creator A5001977114 @default.
• W2154633231 creator A5064417889 @default.
• W2154633231 date "1996-10-01" @default.
• W2154633231 modified "2023-09-27" @default.
• W2154633231 title "Evidence of the Presence of a Specific ATPase Responsible for ATP-initiated Calcification by Matrix Vesicles Isolated from Cartilage and Bone" @default.
• W2154633231 cites W1548847230 @default.
• W2154633231 cites W1565936007 @default.
• W2154633231 cites W1775749144 @default.
• W2154633231 cites W1775897120 @default.
• W2154633231 cites W1965165458 @default.
• W2154633231 cites W1994577132 @default.
• W2154633231 cites W2000219458 @default.
• W2154633231 cites W2004919893 @default.
• W2154633231 cites W2006732343 @default.
• W2154633231 cites W2014564452 @default.
• W2154633231 cites W2014571318 @default.
• W2154633231 cites W2015941566 @default.
• W2154633231 cites W2026059387 @default.
• W2154633231 cites W2031100349 @default.
• W2154633231 cites W2037673705 @default.
• W2154633231 cites W2040724553 @default.
• W2154633231 cites W2050942360 @default.
• W2154633231 cites W2051743902 @default.
• W2154633231 cites W2053058852 @default.
• W2154633231 cites W2056208647 @default.
• W2154633231 cites W2056700455 @default.
• W2154633231 cites W2063790186 @default.
• W2154633231 cites W2069938760 @default.
• W2154633231 cites W2070086332 @default.
• W2154633231 cites W2082266074 @default.
• W2154633231 cites W2088782754 @default.
• W2154633231 cites W2232407802 @default.
• W2154633231 cites W2251650758 @default.
• W2154633231 cites W2267870166 @default.
• W2154633231 cites W4210978097 @default.
• W2154633231 cites W4300936953 @default.
• W2154633231 cites W61595453 @default.
• W2154633231 doi "https://doi.org/10.1074/jbc.271.42.26383" @default.
• W2154633231 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8824294" @default.
• W2154633231 hasPublicationYear "1996" @default.
• W2154633231 type Work @default.
• W2154633231 sameAs 2154633231 @default.
• W2154633231 citedByCount "54" @default.
• W2154633231 countsByYear W21546332312013 @default.
• W2154633231 countsByYear W21546332312015 @default.
• W2154633231 countsByYear W21546332312017 @default.
• W2154633231 countsByYear W21546332312020 @default.
• W2154633231 countsByYear W21546332312023 @default.
• W2154633231 crossrefType "journal-article" @default.
• W2154633231 hasAuthorship W2154633231A5001977114 @default.
• W2154633231 hasAuthorship W2154633231A5064417889 @default.
• W2154633231 hasBestOaLocation W21546332311 @default.
• W2154633231 hasConcept C126322002 @default.
• W2154633231 hasConcept C130316041 @default.
• W2154633231 hasConcept C141315368 @default.
• W2154633231 hasConcept C160160445 @default.
• W2154633231 hasConcept C181199279 @default.
• W2154633231 hasConcept C185592680 @default.
• W2154633231 hasConcept C23265538 @default.
• W2154633231 hasConcept C2777739839 @default.
• W2154633231 hasConcept C2780309369 @default.
• W2154633231 hasConcept C2781116636 @default.
• W2154633231 hasConcept C2992907065 @default.
• W2154633231 hasConcept C41625074 @default.
• W2154633231 hasConcept C55493867 @default.
• W2154633231 hasConcept C71924100 @default.
• W2154633231 hasConcept C94412978 @default.
• W2154633231 hasConceptScore W2154633231C126322002 @default.
• W2154633231 hasConceptScore W2154633231C130316041 @default.
• W2154633231 hasConceptScore W2154633231C141315368 @default.
• W2154633231 hasConceptScore W2154633231C160160445 @default.
• W2154633231 hasConceptScore W2154633231C181199279 @default.
• W2154633231 hasConceptScore W2154633231C185592680 @default.
• W2154633231 hasConceptScore W2154633231C23265538 @default.
• W2154633231 hasConceptScore W2154633231C2777739839 @default.
• W2154633231 hasConceptScore W2154633231C2780309369 @default.
• W2154633231 hasConceptScore W2154633231C2781116636 @default.
• W2154633231 hasConceptScore W2154633231C2992907065 @default.
• W2154633231 hasConceptScore W2154633231C41625074 @default.
• W2154633231 hasConceptScore W2154633231C55493867 @default.
• W2154633231 hasConceptScore W2154633231C71924100 @default.
• W2154633231 hasConceptScore W2154633231C94412978 @default.
• W2154633231 hasIssue "42" @default.
• W2154633231 hasLocation W21546332311 @default.
• W2154633231 hasOpenAccess W2154633231 @default.
• W2154633231 hasPrimaryLocation W21546332311 @default.
• W2154633231 hasRelatedWork W2016340940 @default.
• W2154633231 hasRelatedWork W2025000256 @default.
• W2154633231 hasRelatedWork W2037574386 @default.
• W2154633231 hasRelatedWork W2046124690 @default.
• W2154633231 hasRelatedWork W2056106536 @default.
• W2154633231 hasRelatedWork W2058548582 @default.
• W2154633231 hasRelatedWork W2154633231 @default.
• W2154633231 hasRelatedWork W2187664889 @default.
• W2154633231 hasRelatedWork W2258702507 @default.
• W2154633231 hasRelatedWork W3035864008 @default.

• next