FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2154643923> ?p ?o ?g. }

• W2154643923 endingPage "1811" @default.
• W2154643923 startingPage "1806" @default.
• W2154643923 abstract "ABSTRACT The homodimeric diphtheria toxin repressor (DtxR) uses Fe 2+ as a corepressor, binds to iron-regulated promoters, and negatively regulates the syntheses of diphtheria toxin, corynebacterial siderophore, and several other Corynebacterium diphtheriae products. The crystal structure of DtxR shows that the second domain of each monomer has two binding sites for Fe 2+ or certain other divalent metal ions. In addition, site 1 binds a sulfate or phosphate anion, suggesting that phosphate may function intracellularly as a co-corepressor. The effects of alanine substitutions for selected residues in sites 1 and 2 were determined by measuring the β-galactosidase activities of a tox operator/promoter- lacZ reporter construct in Escherichia coli strains expressing each DtxR variant. Our studies demonstrated that single alanine substitutions for the anion-binding residues in site 1 (R80A, S126A, or N130A) caused severely decreased DtxR activity, similar to the effects of alanine substitutions for metal-binding residues in site 2 (C102A, E105A, or H106A) and greater than the effects of alanine substitutions for metal-binding residues in site 1 (H79A, E83A, or H98A) reported previously by other investigators. Various combinations of alanine substitutions for site 1 and site 2 residues were also analyzed to further elucidate the roles of these cation- and anion-binding ligands in DtxR activity. Furthermore, the interaction between residue E20 in the DNA binding domain and R80 in anion/cation binding site 1 was analyzed, and the E20A variant of DtxR was shown to have a phenotype indistinguishable from that of the R80A variant. Our data demonstrated for the first time that the anion-binding residues R80, S126, and N130 at site 1 are essential for DtxR activity. The data also showed that the interaction of E20 in domain 1 with R80 in domain 2, first revealed by X-ray crystallography in apo-DtxR and holo-DtxR, is a structural feature of DtxR that is important for its repressor activity." @default.
• W2154643923 created "2016-06-24" @default.
• W2154643923 creator A5006728493 @default.
• W2154643923 creator A5023219546 @default.
• W2154643923 creator A5051273903 @default.
• W2154643923 creator A5056154663 @default.
• W2154643923 date "1999-04-01" @default.
• W2154643923 modified "2023-10-03" @default.
• W2154643923 title "Anion-Coordinating Residues at Binding Site 1 Are Essential for the Biological Activity of the Diphtheria Toxin Repressor" @default.
• W2154643923 cites W1505376259 @default.
• W2154643923 cites W1515226138 @default.
• W2154643923 cites W1599629122 @default.
• W2154643923 cites W1607166463 @default.
• W2154643923 cites W1627110571 @default.
• W2154643923 cites W1795554247 @default.
• W2154643923 cites W1969905579 @default.
• W2154643923 cites W1970227811 @default.
• W2154643923 cites W1975813544 @default.
• W2154643923 cites W1998862250 @default.
• W2154643923 cites W2024265765 @default.
• W2154643923 cites W2032387849 @default.
• W2154643923 cites W2037500886 @default.
• W2154643923 cites W2041491105 @default.
• W2154643923 cites W2045517142 @default.
• W2154643923 cites W2049297824 @default.
• W2154643923 cites W2051262420 @default.
• W2154643923 cites W2054941036 @default.
• W2154643923 cites W2060627468 @default.
• W2154643923 cites W2072148144 @default.
• W2154643923 cites W2075090496 @default.
• W2154643923 cites W2083501944 @default.
• W2154643923 cites W2087502952 @default.
• W2154643923 cites W2107991524 @default.
• W2154643923 cites W2114187319 @default.
• W2154643923 cites W2118572661 @default.
• W2154643923 cites W2124865798 @default.
• W2154643923 cites W2126680603 @default.
• W2154643923 cites W2138270253 @default.
• W2154643923 cites W2146183433 @default.
• W2154643923 cites W2151566970 @default.
• W2154643923 cites W2164859504 @default.
• W2154643923 cites W2176699307 @default.
• W2154643923 cites W2178477917 @default.
• W2154643923 cites W4247478626 @default.
• W2154643923 cites W4320399422 @default.
• W2154643923 doi "https://doi.org/10.1128/iai.67.4.1806-1811.1999" @default.
• W2154643923 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/96531" @default.
• W2154643923 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10085021" @default.
• W2154643923 hasPublicationYear "1999" @default.
• W2154643923 type Work @default.
• W2154643923 sameAs 2154643923 @default.
• W2154643923 citedByCount "23" @default.
• W2154643923 countsByYear W21546439232012 @default.
• W2154643923 countsByYear W21546439232013 @default.
• W2154643923 countsByYear W21546439232014 @default.
• W2154643923 countsByYear W21546439232016 @default.
• W2154643923 countsByYear W21546439232020 @default.
• W2154643923 countsByYear W21546439232022 @default.
• W2154643923 countsByYear W21546439232023 @default.
• W2154643923 crossrefType "journal-article" @default.
• W2154643923 hasAuthorship W2154643923A5006728493 @default.
• W2154643923 hasAuthorship W2154643923A5023219546 @default.
• W2154643923 hasAuthorship W2154643923A5051273903 @default.
• W2154643923 hasAuthorship W2154643923A5056154663 @default.
• W2154643923 hasBestOaLocation W21546439231 @default.
• W2154643923 hasConcept C104317684 @default.
• W2154643923 hasConcept C107824862 @default.
• W2154643923 hasConcept C146777309 @default.
• W2154643923 hasConcept C158448853 @default.
• W2154643923 hasConcept C181199279 @default.
• W2154643923 hasConcept C185592680 @default.
• W2154643923 hasConcept C203014093 @default.
• W2154643923 hasConcept C22070199 @default.
• W2154643923 hasConcept C2777367657 @default.
• W2154643923 hasConcept C2779856020 @default.
• W2154643923 hasConcept C2780572238 @default.
• W2154643923 hasConcept C2781288345 @default.
• W2154643923 hasConcept C33057221 @default.
• W2154643923 hasConcept C41183919 @default.
• W2154643923 hasConcept C515207424 @default.
• W2154643923 hasConcept C547475151 @default.
• W2154643923 hasConcept C55493867 @default.
• W2154643923 hasConcept C71240020 @default.
• W2154643923 hasConcept C86339819 @default.
• W2154643923 hasConcept C86803240 @default.
• W2154643923 hasConceptScore W2154643923C104317684 @default.
• W2154643923 hasConceptScore W2154643923C107824862 @default.
• W2154643923 hasConceptScore W2154643923C146777309 @default.
• W2154643923 hasConceptScore W2154643923C158448853 @default.
• W2154643923 hasConceptScore W2154643923C181199279 @default.
• W2154643923 hasConceptScore W2154643923C185592680 @default.
• W2154643923 hasConceptScore W2154643923C203014093 @default.
• W2154643923 hasConceptScore W2154643923C22070199 @default.
• W2154643923 hasConceptScore W2154643923C2777367657 @default.
• W2154643923 hasConceptScore W2154643923C2779856020 @default.
• W2154643923 hasConceptScore W2154643923C2780572238 @default.
• W2154643923 hasConceptScore W2154643923C2781288345 @default.
• W2154643923 hasConceptScore W2154643923C33057221 @default.

• next