FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2154680856> ?p ?o ?g. }

• W2154680856 endingPage "334" @default.
• W2154680856 startingPage "326" @default.
• W2154680856 abstract "In this study, a modified method of the conventional RNA dot-blot hybridization was established, by replacing (32)P labels with CY5 labels and replacing nylon membranes with positive-charged glass slides, for detecting plant RNA viruses and a viroid. The modified RNA dot-blot hybridization method was named glass slide hybridization. The optimum efficiency of RNA binding onto the surfaces of activated glass slide was achieved using aminosilane-coated glass slide as a solid matrix and 5xsaline sodium citrate (SSC) as a spotting solution. Using a CY5-labeled DNA probe prepared through PCR amplification, the optimized glass slide hybridization could detect as little as 1.71 pg of tobacco mosaic virus (TMV) RNA. The sensitivity of the modified method was four times that of dot-blot hybridization on nylon membrane with a (32)P-labeled probe. The absence of false positive within the genus Potyvirus [potato virus A, potato virus Y (PVY) and zucchini yellow mosaic virus] showed that this method was highly specific. Furthermore, potato spindle tuber viroid (PSTVd) was also detected specifically. A test of 40 field potato samples showed that this method was equivalent to the conventional dot-blot hybridization for detecting PVY and PSTVd. To our knowledge, this is the first report of using dot-blot hybridization on glass slides with fluorescent-labeled probes for detecting plant RNA viruses and a viroid." @default.
• W2154680856 created "2016-06-24" @default.
• W2154680856 creator A5011487190 @default.
• W2154680856 creator A5024871063 @default.
• W2154680856 creator A5027548955 @default.
• W2154680856 creator A5063661455 @default.
• W2154680856 creator A5085831921 @default.
• W2154680856 date "2007-05-01" @default.
• W2154680856 modified "2023-09-28" @default.
• W2154680856 title "Highly Sensitive Fluorescent-labeled Probes and Glass Slide Hybridization for the Detection of Plant RNA Viruses and a Viroid" @default.
• W2154680856 cites W1964887833 @default.
• W2154680856 cites W1985823148 @default.
• W2154680856 cites W1988053043 @default.
• W2154680856 cites W1990366516 @default.
• W2154680856 cites W1993163345 @default.
• W2154680856 cites W1997357720 @default.
• W2154680856 cites W2006269250 @default.
• W2154680856 cites W2008210873 @default.
• W2154680856 cites W2015341941 @default.
• W2154680856 cites W2027177386 @default.
• W2154680856 cites W2032144267 @default.
• W2154680856 cites W2033380151 @default.
• W2154680856 cites W2039020873 @default.
• W2154680856 cites W2043744845 @default.
• W2154680856 cites W2056324404 @default.
• W2154680856 cites W2058317097 @default.
• W2154680856 cites W2066537782 @default.
• W2154680856 cites W2071254940 @default.
• W2154680856 cites W2076377242 @default.
• W2154680856 cites W2098947584 @default.
• W2154680856 cites W2131188188 @default.
• W2154680856 cites W2133247102 @default.
• W2154680856 cites W2161233925 @default.
• W2154680856 cites W2351122600 @default.
• W2154680856 cites W2362073006 @default.
• W2154680856 cites W2365849387 @default.
• W2154680856 cites W2742386576 @default.
• W2154680856 doi "https://doi.org/10.1111/j.1745-7270.2007.00290.x" @default.
• W2154680856 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17492129" @default.
• W2154680856 hasPublicationYear "2007" @default.
• W2154680856 type Work @default.
• W2154680856 sameAs 2154680856 @default.
• W2154680856 citedByCount "9" @default.
• W2154680856 countsByYear W21546808562013 @default.
• W2154680856 countsByYear W21546808562014 @default.
• W2154680856 countsByYear W21546808562017 @default.
• W2154680856 countsByYear W21546808562020 @default.
• W2154680856 countsByYear W21546808562022 @default.
• W2154680856 crossrefType "journal-article" @default.
• W2154680856 hasAuthorship W2154680856A5011487190 @default.
• W2154680856 hasAuthorship W2154680856A5024871063 @default.
• W2154680856 hasAuthorship W2154680856A5027548955 @default.
• W2154680856 hasAuthorship W2154680856A5063661455 @default.
• W2154680856 hasAuthorship W2154680856A5085831921 @default.
• W2154680856 hasConcept C104317684 @default.
• W2154680856 hasConcept C105580179 @default.
• W2154680856 hasConcept C109110057 @default.
• W2154680856 hasConcept C130197536 @default.
• W2154680856 hasConcept C130840561 @default.
• W2154680856 hasConcept C144707691 @default.
• W2154680856 hasConcept C153911025 @default.
• W2154680856 hasConcept C159047783 @default.
• W2154680856 hasConcept C2522874641 @default.
• W2154680856 hasConcept C2776552150 @default.
• W2154680856 hasConcept C2776625791 @default.
• W2154680856 hasConcept C2780504191 @default.
• W2154680856 hasConcept C2780757758 @default.
• W2154680856 hasConcept C2780781446 @default.
• W2154680856 hasConcept C54871578 @default.
• W2154680856 hasConcept C552990157 @default.
• W2154680856 hasConcept C55493867 @default.
• W2154680856 hasConcept C67705224 @default.
• W2154680856 hasConcept C81439078 @default.
• W2154680856 hasConcept C86803240 @default.
• W2154680856 hasConceptScore W2154680856C104317684 @default.
• W2154680856 hasConceptScore W2154680856C105580179 @default.
• W2154680856 hasConceptScore W2154680856C109110057 @default.
• W2154680856 hasConceptScore W2154680856C130197536 @default.
• W2154680856 hasConceptScore W2154680856C130840561 @default.
• W2154680856 hasConceptScore W2154680856C144707691 @default.
• W2154680856 hasConceptScore W2154680856C153911025 @default.
• W2154680856 hasConceptScore W2154680856C159047783 @default.
• W2154680856 hasConceptScore W2154680856C2522874641 @default.
• W2154680856 hasConceptScore W2154680856C2776552150 @default.
• W2154680856 hasConceptScore W2154680856C2776625791 @default.
• W2154680856 hasConceptScore W2154680856C2780504191 @default.
• W2154680856 hasConceptScore W2154680856C2780757758 @default.
• W2154680856 hasConceptScore W2154680856C2780781446 @default.
• W2154680856 hasConceptScore W2154680856C54871578 @default.
• W2154680856 hasConceptScore W2154680856C552990157 @default.
• W2154680856 hasConceptScore W2154680856C55493867 @default.
• W2154680856 hasConceptScore W2154680856C67705224 @default.
• W2154680856 hasConceptScore W2154680856C81439078 @default.
• W2154680856 hasConceptScore W2154680856C86803240 @default.
• W2154680856 hasIssue "5" @default.
• W2154680856 hasLocation W21546808561 @default.
• W2154680856 hasLocation W21546808562 @default.
• W2154680856 hasOpenAccess W2154680856 @default.

• next