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- W2154752606 abstract "A Campylobacter jejuni gene encoding a homologue of the flagellar biosynthesis gene flhB was identified downstream of the peroxide stress defence gene ahpC. Insertional mutagenesis of the flhB gene rendered C. jejuni non-motile, with most cells aflagellate, although a small number expressed truncated flagella. The absence of FlhB also appeared to affect cell shape, as the majority of cells were straight rather than curved rods. Transcription of the flagellin gene flaA was significantly reduced in the C. jejuni flhB mutants, which also did not express significant amounts of flagellin proteins, indicating that FlhB is an essential protein for subsequent expression of flagellar genes. The transcription start site of the flhB gene, as determined by primer extension, was located 91 bp upstream of the flhB start codon, but no recognizable promoter sequence could be identified immediately upstream of this transcription start site. Transcriptional flhB::lacZ reporter gene fusions confirmed that the flhB gene has its own promoter region, is expressed at very low levels and is transcribed independently of ahpC, and that its transcription is not regulated by iron or growth phase." @default.
- W2154752606 created "2016-06-24" @default.
- W2154752606 creator A5019638261 @default.
- W2154752606 creator A5053442760 @default.
- W2154752606 creator A5061733701 @default.
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- W2154752606 date "2002-06-01" @default.
- W2154752606 modified "2023-09-26" @default.
- W2154752606 title "Mutational and transcriptional analysis of the Campylobacter jejuni flagellar biosynthesis gene flhB" @default.
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- W2154752606 doi "https://doi.org/10.1099/00221287-148-6-1679" @default.
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