FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2154805215> ?p ?o ?g. }

• W2154805215 endingPage "355" @default.
• W2154805215 startingPage "347" @default.
• W2154805215 abstract "We previously reported that the time-dependent relative changes of transepithelial electrical resistance (TEER) after exposing four different chemicals to a human corneal epithelium (HCE) model were well correlated to the potential of ocular irritancy. Meanwhile, we recently developed a collagen vitrigel membrane (CVM) chamber possessing a scaffold composed of high-density collagen fibrils equivalent to connective tissues in vivo as a three-dimensional culture tool. The CVM chamber is useful for biomedical assays and immunohistology using cryosections that are inappropriate to be performed using the conventional Millicell chamber with a polyethylene terephthalate membrane. In this study, we aimed to develop a new eye irritancy test (EIT) method called “Vitrigel-EIT method” that can facilitate to briefly and accurately estimate the widespread irritancy of test chemicals by applying the TEER assay system to a HCE model fabricated in the CVM chamber. HCE-T cells (a HCE-derived cell strain) were cultured in the CVM chamber for 6 days, and consequently, the Vitrigel-HCE model possessing the following characteristics of HCE in vivo was formed: six cell layers with specific protein expressions and their barrier function. Time-dependent profiles of TEER values after exposing 30 test chemicals to the HCE model were converted into the scores of three indexes (time lag, intensity, and plateau level), and each chemical was successfully classified into irritant or nonirritant category by utilizing the criteria for the indexes, resulting in the excellent correlation with Globally Harmonized System of Classification and Labelling of Chemicals (GHS) classification (sensitivity: 100%, specificity: 75%, accuracy: 90%). These data suggest that the widespread eye irritancy of chemicals can be predicted without false negatives by the Vitrigel-EIT method. Interestingly, the disruption of tight junctions was immunohistologically observed after exposing not only irritants but also three compounds classified as nonirritant by GHS but found positive in our Vitrigel-EIT method confirming a possible mild irritant property." @default.
• W2154805215 created "2016-06-24" @default.
• W2154805215 creator A5025030198 @default.
• W2154805215 creator A5045326528 @default.
• W2154805215 creator A5075344478 @default.
• W2154805215 date "2013-07-20" @default.
• W2154805215 modified "2023-10-17" @default.
• W2154805215 title "Vitrigel-Eye Irritancy Test Method Using HCE-T Cells" @default.
• W2154805215 cites W1965083225 @default.
• W2154805215 cites W1970107624 @default.
• W2154805215 cites W1975990337 @default.
• W2154805215 cites W1976352504 @default.
• W2154805215 cites W1982381849 @default.
• W2154805215 cites W2000334797 @default.
• W2154805215 cites W2021420769 @default.
• W2154805215 cites W2022620728 @default.
• W2154805215 cites W2022635325 @default.
• W2154805215 cites W2022879923 @default.
• W2154805215 cites W2026545622 @default.
• W2154805215 cites W2031151230 @default.
• W2154805215 cites W2036170607 @default.
• W2154805215 cites W2042216373 @default.
• W2154805215 cites W2061076031 @default.
• W2154805215 cites W2063321046 @default.
• W2154805215 cites W2073887351 @default.
• W2154805215 cites W2102575814 @default.
• W2154805215 cites W4237942409 @default.
• W2154805215 doi "https://doi.org/10.1093/toxsci/kft159" @default.
• W2154805215 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23872712" @default.
• W2154805215 hasPublicationYear "2013" @default.
• W2154805215 type Work @default.
• W2154805215 sameAs 2154805215 @default.
• W2154805215 citedByCount "30" @default.
• W2154805215 countsByYear W21548052152014 @default.
• W2154805215 countsByYear W21548052152015 @default.
• W2154805215 countsByYear W21548052152016 @default.
• W2154805215 countsByYear W21548052152017 @default.
• W2154805215 countsByYear W21548052152018 @default.
• W2154805215 countsByYear W21548052152019 @default.
• W2154805215 countsByYear W21548052152020 @default.
• W2154805215 countsByYear W21548052152021 @default.
• W2154805215 countsByYear W21548052152022 @default.
• W2154805215 countsByYear W21548052152023 @default.
• W2154805215 crossrefType "journal-article" @default.
• W2154805215 hasAuthorship W2154805215A5025030198 @default.
• W2154805215 hasAuthorship W2154805215A5045326528 @default.
• W2154805215 hasAuthorship W2154805215A5075344478 @default.
• W2154805215 hasBestOaLocation W21548052151 @default.
• W2154805215 hasConcept C118487528 @default.
• W2154805215 hasConcept C12554922 @default.
• W2154805215 hasConcept C136229726 @default.
• W2154805215 hasConcept C142724271 @default.
• W2154805215 hasConcept C150903083 @default.
• W2154805215 hasConcept C159985019 @default.
• W2154805215 hasConcept C185592680 @default.
• W2154805215 hasConcept C192562407 @default.
• W2154805215 hasConcept C207001950 @default.
• W2154805215 hasConcept C2776882836 @default.
• W2154805215 hasConcept C2780017307 @default.
• W2154805215 hasConcept C41625074 @default.
• W2154805215 hasConcept C518705261 @default.
• W2154805215 hasConcept C55493867 @default.
• W2154805215 hasConcept C71924100 @default.
• W2154805215 hasConcept C86803240 @default.
• W2154805215 hasConceptScore W2154805215C118487528 @default.
• W2154805215 hasConceptScore W2154805215C12554922 @default.
• W2154805215 hasConceptScore W2154805215C136229726 @default.
• W2154805215 hasConceptScore W2154805215C142724271 @default.
• W2154805215 hasConceptScore W2154805215C150903083 @default.
• W2154805215 hasConceptScore W2154805215C159985019 @default.
• W2154805215 hasConceptScore W2154805215C185592680 @default.
• W2154805215 hasConceptScore W2154805215C192562407 @default.
• W2154805215 hasConceptScore W2154805215C207001950 @default.
• W2154805215 hasConceptScore W2154805215C2776882836 @default.
• W2154805215 hasConceptScore W2154805215C2780017307 @default.
• W2154805215 hasConceptScore W2154805215C41625074 @default.
• W2154805215 hasConceptScore W2154805215C518705261 @default.
• W2154805215 hasConceptScore W2154805215C55493867 @default.
• W2154805215 hasConceptScore W2154805215C71924100 @default.
• W2154805215 hasConceptScore W2154805215C86803240 @default.
• W2154805215 hasIssue "2" @default.
• W2154805215 hasLocation W21548052151 @default.
• W2154805215 hasLocation W21548052152 @default.
• W2154805215 hasOpenAccess W2154805215 @default.
• W2154805215 hasPrimaryLocation W21548052151 @default.
• W2154805215 hasRelatedWork W114115982 @default.
• W2154805215 hasRelatedWork W1995156228 @default.
• W2154805215 hasRelatedWork W2093264097 @default.
• W2154805215 hasRelatedWork W2891226631 @default.
• W2154805215 hasRelatedWork W3033862266 @default.
• W2154805215 hasRelatedWork W3131279331 @default.
• W2154805215 hasRelatedWork W4235259251 @default.
• W2154805215 hasRelatedWork W4297194660 @default.
• W2154805215 hasRelatedWork W4308572702 @default.
• W2154805215 hasRelatedWork W4321435646 @default.
• W2154805215 hasVolume "135" @default.
• W2154805215 isParatext "false" @default.
• W2154805215 isRetracted "false" @default.

• next