FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2154986269> ?p ?o ?g. }

• W2154986269 endingPage "1824" @default.
• W2154986269 startingPage "1811" @default.
• W2154986269 abstract "In fission yeast, siRNA is generated from pericentromeric noncoding RNA by the RNAi machinery. siRNA synthesis and heterochromatin formation are interdependent, forming a self-reinforcing loop on chromatin. In this system, siRNA is amplified by the RNA-dependent RNA polymerase complex (RDRC) and the endoribonuclease Dcr1, which synthesizes dsRNA and processes the dsRNA, respectively. The amplification is essential for stable heterochromatin formation. Here, a novel gene, dsh1 + ( defect of the gene silencing at centromeric heterochromatin ), is identified as an essential component of RNAi-directed heterochromatin assembly. Loss of dsh1 + abolishes normal RNAi function and heterochromatic gene silencing at pericentromeres. Dsh1 interacts with Dcr1 and RDRC and couples the reactions of both proteins to the effective production of siRNA in vivo. Dsh1 binds to heterochromatin in the absence of RDRC, while RDRC requires Dsh1 for its chromatin-binding activity, suggesting that Dsh1 recruits RDRC to chromatin. Immunofluorescence analysis shows that Dsh1 forms foci at the nuclear periphery, and some Dsh1 foci colocalize with Dcr1 and RDRC. Dsh1 is required for the colocalization of Dcr1 and RDRC. Moreover, loss of the nuclear periphery localization of Dsh1 abolishes Dsh1 function. Taken together, these results suggest that Dsh1 assembles the RNAi machinery on heterochromatin and forms a perinuclear compartment for amplification of heterochromatic siRNA." @default.
• W2154986269 created "2016-06-24" @default.
• W2154986269 creator A5000720333 @default.
• W2154986269 creator A5005417954 @default.
• W2154986269 creator A5009441392 @default.
• W2154986269 creator A5013700795 @default.
• W2154986269 date "2012-08-15" @default.
• W2154986269 modified "2023-10-02" @default.
• W2154986269 title "A novel RNAi protein, Dsh1, assembles RNAi machinery on chromatin to amplify heterochromatic siRNA" @default.
• W2154986269 cites W1479904481 @default.
• W2154986269 cites W1528037187 @default.
• W2154986269 cites W1837587701 @default.
• W2154986269 cites W1964271596 @default.
• W2154986269 cites W1968882472 @default.
• W2154986269 cites W1971279688 @default.
• W2154986269 cites W1978197643 @default.
• W2154986269 cites W1989753542 @default.
• W2154986269 cites W1990265320 @default.
• W2154986269 cites W1992506108 @default.
• W2154986269 cites W1999284470 @default.
• W2154986269 cites W2003518550 @default.
• W2154986269 cites W2004658265 @default.
• W2154986269 cites W2008304407 @default.
• W2154986269 cites W2015735754 @default.
• W2154986269 cites W2029234227 @default.
• W2154986269 cites W2037541952 @default.
• W2154986269 cites W2040234712 @default.
• W2154986269 cites W2040708394 @default.
• W2154986269 cites W2042812035 @default.
• W2154986269 cites W2046393794 @default.
• W2154986269 cites W2046820586 @default.
• W2154986269 cites W2047478918 @default.
• W2154986269 cites W2052770408 @default.
• W2154986269 cites W2059178026 @default.
• W2154986269 cites W2063206680 @default.
• W2154986269 cites W2063612621 @default.
• W2154986269 cites W2070122527 @default.
• W2154986269 cites W2089266451 @default.
• W2154986269 cites W2094431101 @default.
• W2154986269 cites W2099183055 @default.
• W2154986269 cites W2110107454 @default.
• W2154986269 cites W2117771255 @default.
• W2154986269 cites W2138094969 @default.
• W2154986269 cites W2139745949 @default.
• W2154986269 cites W2141452262 @default.
• W2154986269 cites W2143313547 @default.
• W2154986269 cites W2151809557 @default.
• W2154986269 cites W2161729182 @default.
• W2154986269 cites W2161976991 @default.
• W2154986269 cites W2162996117 @default.
• W2154986269 cites W2165071626 @default.
• W2154986269 cites W2169577566 @default.
• W2154986269 cites W2181998616 @default.
• W2154986269 cites W2194986544 @default.
• W2154986269 cites W2336109233 @default.
• W2154986269 doi "https://doi.org/10.1101/gad.190272.112" @default.
• W2154986269 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3465744" @default.
• W2154986269 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22895252" @default.
• W2154986269 hasPublicationYear "2012" @default.
• W2154986269 type Work @default.
• W2154986269 sameAs 2154986269 @default.
• W2154986269 citedByCount "22" @default.
• W2154986269 countsByYear W21549862692013 @default.
• W2154986269 countsByYear W21549862692014 @default.
• W2154986269 countsByYear W21549862692015 @default.
• W2154986269 countsByYear W21549862692016 @default.
• W2154986269 countsByYear W21549862692017 @default.
• W2154986269 countsByYear W21549862692018 @default.
• W2154986269 countsByYear W21549862692022 @default.
• W2154986269 countsByYear W21549862692023 @default.
• W2154986269 crossrefType "journal-article" @default.
• W2154986269 hasAuthorship W2154986269A5000720333 @default.
• W2154986269 hasAuthorship W2154986269A5005417954 @default.
• W2154986269 hasAuthorship W2154986269A5009441392 @default.
• W2154986269 hasAuthorship W2154986269A5013700795 @default.
• W2154986269 hasBestOaLocation W21549862691 @default.
• W2154986269 hasConcept C103435993 @default.
• W2154986269 hasConcept C104317684 @default.
• W2154986269 hasConcept C119056186 @default.
• W2154986269 hasConcept C153911025 @default.
• W2154986269 hasConcept C161223559 @default.
• W2154986269 hasConcept C166703698 @default.
• W2154986269 hasConcept C179138559 @default.
• W2154986269 hasConcept C2191507 @default.
• W2154986269 hasConcept C22615655 @default.
• W2154986269 hasConcept C54355233 @default.
• W2154986269 hasConcept C67705224 @default.
• W2154986269 hasConcept C83640560 @default.
• W2154986269 hasConcept C86803240 @default.
• W2154986269 hasConcept C92149661 @default.
• W2154986269 hasConcept C95444343 @default.
• W2154986269 hasConceptScore W2154986269C103435993 @default.
• W2154986269 hasConceptScore W2154986269C104317684 @default.
• W2154986269 hasConceptScore W2154986269C119056186 @default.
• W2154986269 hasConceptScore W2154986269C153911025 @default.
• W2154986269 hasConceptScore W2154986269C161223559 @default.
• W2154986269 hasConceptScore W2154986269C166703698 @default.
• W2154986269 hasConceptScore W2154986269C179138559 @default.

• next