FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155015939> ?p ?o ?g. }

• W2155015939 endingPage "29799" @default.
• W2155015939 startingPage "29794" @default.
• W2155015939 abstract "Photoreceptor-specific expression of rhodopsin is mediated by multiple cis-acting elements in the proximal promoter region. NRL (neural retina leucine zipper) and CRX (cone rod homeobox) proteins bind to the adjacent NRE and Ret-4 sites, respectively, within this region. Although NRL and CRX are each individually able to induce rhodopsin promoter activity, when expressed together they exhibit transcriptional synergy in rhodopsin promoter activation. Using the yeast two-hybrid method and glutathione S-transferase pull-down assays, we demonstrate that the leucine zipper of NRL can physically interact with CRX. Deletion analysis revealed that the CRX homeodomain (CRX-HD) plays an important role in the interaction with the NRL leucine zipper. Although binding with the CRX-HD alone was weak, a strong interaction was detected when flanking regions including the glutamine-rich and the basic regions that follow the HD were included. A reciprocal deletion analysis showed that the leucine zipper of NRL is required for interaction with CRX-HD. Two disease-causing mutations in CRX-HD (R41W and R90W) that exhibit reduced DNA binding and transcriptional synergy also decrease its interaction with NRL. These studies suggest novel possibilities for protein-protein interaction between two conserved DNA-binding motifs and imply that cross-talk among distinct regulatory pathways contributes to the establishment and maintenance of photoreceptor function. Photoreceptor-specific expression of rhodopsin is mediated by multiple cis-acting elements in the proximal promoter region. NRL (neural retina leucine zipper) and CRX (cone rod homeobox) proteins bind to the adjacent NRE and Ret-4 sites, respectively, within this region. Although NRL and CRX are each individually able to induce rhodopsin promoter activity, when expressed together they exhibit transcriptional synergy in rhodopsin promoter activation. Using the yeast two-hybrid method and glutathione S-transferase pull-down assays, we demonstrate that the leucine zipper of NRL can physically interact with CRX. Deletion analysis revealed that the CRX homeodomain (CRX-HD) plays an important role in the interaction with the NRL leucine zipper. Although binding with the CRX-HD alone was weak, a strong interaction was detected when flanking regions including the glutamine-rich and the basic regions that follow the HD were included. A reciprocal deletion analysis showed that the leucine zipper of NRL is required for interaction with CRX-HD. Two disease-causing mutations in CRX-HD (R41W and R90W) that exhibit reduced DNA binding and transcriptional synergy also decrease its interaction with NRL. These studies suggest novel possibilities for protein-protein interaction between two conserved DNA-binding motifs and imply that cross-talk among distinct regulatory pathways contributes to the establishment and maintenance of photoreceptor function. rhodopsin proximal promoter region basic leucine zipper glutathioneS-transferase bovine CRX homeodomain Gene activation is a stringently controlled process, involving combinatorial and cooperative action of multiple regulatory proteins with promoter and enhancer DNA elements (1Ptashne M. Gann A. Nature. 1997; 386: 569-577Crossref PubMed Scopus (945) Google Scholar, 2Kuras L. Struhl K. Nature. 1999; 389: 609-613Crossref Scopus (399) Google Scholar, 3Li X.-L. Virbasius A. Zhu X. Green M.R. Nature. 1999; 389: 605-609Crossref Scopus (206) Google Scholar, 4Goodrich J.A. Cutler G. Tijan R. Cell. 1996; 84: 825-830Abstract Full Text Full Text PDF PubMed Scopus (185) Google Scholar, 5Leftsin J.A. Yamamoto K.R. Nature. 1998; 392: 885-888Crossref PubMed Scopus (439) Google Scholar, 6Blackwood E.M. Kadonaga J.T. Science. 1998; 281: 60-63Crossref PubMed Scopus (619) Google Scholar). Recent studies, including reconstitution experiments, have suggested that target specificity and transcriptional synergy are achieved by specific and precise interactions among various activator proteins during the assembly of higher order nucleoprotein complexes, including the “enhanceosome” (7Tijan R. Maniatis T. Cell. 1994; 77: 5-8Abstract Full Text PDF PubMed Scopus (955) Google Scholar, 8Grosschedl R. Curr. Opin. Cell Biol. 1995; 7: 362-370Crossref PubMed Scopus (151) Google Scholar, 9Kim T.K. Maniatis T. Mol. Cell. 1997; 1: 119-129Abstract Full Text Full Text PDF PubMed Scopus (300) Google Scholar, 10Carey M. Cell. 1998; 92: 5-8Abstract Full Text Full Text PDF PubMed Scopus (455) Google Scholar, 11Merika M. Williams A.J. Chen G. Collins T. Thanos D. Mol. Cell. 1998; 1: 277-287Abstract Full Text Full Text PDF PubMed Scopus (367) Google Scholar). The elucidation of these protein-protein and protein-DNA interactions is critical to our understanding of the mechanisms of cell type- and tissue-specific gene expression. Generation of multiple neuronal cell types during retinal development is an evolutionarily conserved biological process, which offers a convenient model system to investigate tissue-specific gene regulation. More than 30 transcription factors representing several classes of DNA-binding proteins are expressed in developing and mature mammalian retina; nevertheless, the precise function of a majority of these proteins remains to be elucidated (12Freund C. Horsford D.J. McInnes R.R. Hum. Mol. Gen. 1996; 5: 1471-1488Crossref PubMed Google Scholar, 13Mathers P.H. Grinsberg A. Makon K.A. Jamrich M. Nature. 1997; 387: 603-607Crossref PubMed Scopus (584) Google Scholar, 14Cepko C.L. Austin C.P. Yang X. Alexiades M. Ezzeddine D. Proc. Natl. Acad. Sci. U. S. A. 1996; 93: 589-595Crossref PubMed Scopus (842) Google Scholar). Rhodopsin, the G-protein-coupled light receptor, is expressed specifically in the rod photoreceptors of retina and is a pivotal protein for visual function. Its expression is correlated to rod differentiation and maintained at high levels afterward, throughout life (15Morrow E.M. Furukawa T. Cepko C.L. Trends Cell Biol. 1998; 8: 353-358Abstract Full Text Full Text PDF PubMed Scopus (78) Google Scholar). Altered expression of rhodopsin and mutations that affect its function in mature rods result in retinal degeneration (15Morrow E.M. Furukawa T. Cepko C.L. Trends Cell Biol. 1998; 8: 353-358Abstract Full Text Full Text PDF PubMed Scopus (78) Google Scholar, 16Rattner A. Sun H. Nathans J. Annu. Rev. Gen. 1999; 33: 89-131Crossref PubMed Scopus (197) Google Scholar). Regulation of rhodopsin expression is primarily at the level of transcription and is mediated by two distinct regions: a proximal sequence from −176 to +70 bp, which determines photoreceptor specificity (called the rhodopsin proximal promoter region (RPPR)),1 and another more upstream region required for high level expression (called the rhodopsin enhancer region) (17Zack D.J. Bennet J. Wang Y. Davenport C. Klaunberg B. Gearhart J. Nathans J. Neuron. 1991; 6: 187-199Abstract Full Text PDF PubMed Scopus (181) Google Scholar, 18Nathans J. Biochemistry. 1992; 31: 4923-4931Crossref PubMed Scopus (166) Google Scholar, 19Timmers A.M. Newton B.R. Hauswirth W.W. Exp. Eye. Res. 1993; 56: 257-265Crossref PubMed Scopus (32) Google Scholar, 20Treisman J.E. Morabito M.A. Barnstable C.J. Mol. Cell. Biol. 1988; 8: 1570-1579Crossref PubMed Scopus (93) Google Scholar, 21Lem J. Applebury M.L. Falk J.D. Flannery J.G. Simon M.I. Neuron. 1991; 6: 201-210Abstract Full Text PDF PubMed Scopus (156) Google Scholar, 22Nie Z. Chen S. Kumar R. Zack D.J. J. Biol. Chem. 1996; 271: 2667-2675Abstract Full Text Full Text PDF PubMed Scopus (46) Google Scholar). A number of DNA sequence elements that bind to nuclear proteins have been identified within RPPR; these include Ret-1/PCE-1 (23Morabito M.A., Yu, X. Barnstable C.J. J. Biol. Chem. 1991; 266: 9667-9672Abstract Full Text PDF PubMed Google Scholar, 24Kikuchi T. Raju K. Breitman M.L. Shinohara T. Mol. Cell. Biol. 1993; 13: 4400-4408Crossref PubMed Scopus (105) Google Scholar), BAT-1 (25DesJardin L.E. Hauswirth W.W. Invest. Ophthalmol. Vis. Sci. 1996; 37: 154-165PubMed Google Scholar), eopsin-1 (26Ahmad I. Dev. Brain. Res. 1995; 90: 184-189Crossref PubMed Scopus (51) Google Scholar), Ret-4 (27Chen S. Zack D.J. J. Biol. Chem. 1996; 271: 28549-28557Abstract Full Text Full Text PDF PubMed Scopus (57) Google Scholar), and NRE (28Rehemtulla A. Warwar R. Kumar R. Ji X. Zack D.J. Swaroop A. Proc. Natl. Acad. Sci. U. S. A. 1996; 93: 191-195Crossref PubMed Scopus (186) Google Scholar) (Fig. 1). NRL, a basic leucine zipper (bZIP) protein of the Maf subfamily (29Swaroop A. Xu J. Pawar H. Jackson A. Skolnick C. Agarwal N. Proc. Natl. Acad. Sci. U. S. A. 1992; 89: 266-270Crossref PubMed Scopus (269) Google Scholar), was the first transcription factor shown to bind to NRE in the RPPR region and transactivate the rhodopsin promoter in cultured cells (28Rehemtulla A. Warwar R. Kumar R. Ji X. Zack D.J. Swaroop A. Proc. Natl. Acad. Sci. U. S. A. 1996; 93: 191-195Crossref PubMed Scopus (186) Google Scholar, 30Kumar R. Chen S. Scheurer D. Wang Q. Duh E. Sung C. Rehemtulla A. Swaroop A. Adler R. Zack D.J. J. Biol. Chem. 1996; 271: 29612-29618Abstract Full Text Full Text PDF PubMed Scopus (128) Google Scholar). Soon thereafter, CRX, a photoreceptor-specific paired-like homeodomain protein, was identified as the Ret-4 and BAT-1 binding protein by yeast one-hybrid screening and shown to activate the promoters of rhodopsin and other retinal genes (31Chen S. Wang Q. Nie Z. Sun H. Lennon G. Copeland N.G. Gilbert J. Jenkins N.A. Zack D.J. Neuron. 1997; 19: 1017-1030Abstract Full Text Full Text PDF PubMed Scopus (577) Google Scholar). NRL and CRX demonstrated transcriptional synergy in rhodopsin promoter activation when transfected together in cultured cells (31Chen S. Wang Q. Nie Z. Sun H. Lennon G. Copeland N.G. Gilbert J. Jenkins N.A. Zack D.J. Neuron. 1997; 19: 1017-1030Abstract Full Text Full Text PDF PubMed Scopus (577) Google Scholar). Recently, the Ret-1/PCE-1 element was shown to bind two other homeodomain proteins, Erx (32Martinez J.A. Barnstable C.J. Biochem. Biophys. Res. Commun. 1998; 250: 175-180Crossref PubMed Scopus (27) Google Scholar) and Rx (33Kimura A. Singh D. Wawrousek E.F. Kikuchi M. Nakamura M. Shinohara T. J. Biol. Chem. 2000; 275: 1152-1160Abstract Full Text Full Text PDF PubMed Scopus (118) Google Scholar). During embryonic development in mice, Nrl transcripts are detected in all postmitotic neurons and lens; however, its expression becomes restricted primarily to the retinal photoreceptors in the adult (34Liu Q. Ji X. Breitman M.L. Hitchcock P.F. Swaroop A. Oncogene. 1996; 12: 207-211PubMed Google Scholar). CRX is expressed specifically in photoreceptors and pinealocytes and plays a significant role during photoreceptor differentiation (31Chen S. Wang Q. Nie Z. Sun H. Lennon G. Copeland N.G. Gilbert J. Jenkins N.A. Zack D.J. Neuron. 1997; 19: 1017-1030Abstract Full Text Full Text PDF PubMed Scopus (577) Google Scholar, 35Furukawa T. Morrow E.M. Cepko C.L. Cell. 1997; 91: 531-541Abstract Full Text Full Text PDF PubMed Scopus (719) Google Scholar,36Furukawa T. Morrow E.M. Li T. Davis F.C. Cepko C.L. Nat. Genet. 1999; 23: 466-470Crossref PubMed Scopus (446) Google Scholar). Consistent with their role in rhodopsin regulation, detection of NRL and CRX transcripts precedes rhodopsin expression during rod development in mammals. In addition, mutations in the humanCRX and NRL genes have been identified in retinopathies, and these mutations result in altered transcriptional synergy in rhodopsin promoter activation assays (37Freund C.L. Gregory-Evans C.Y. Furukawa T. Papaioannou M. Looser J. Ploder L. Bellingham J. Ng D. Herbrick J.-A.S. Duncan A. Scherer S.W. Tsui L.-C. Loutradis-Anagnostou A. Jacobson S.G. Cepko C.L. Bhattacharya S.S. McInnes R.R. Cell. 1997; 91: 543-553Abstract Full Text Full Text PDF PubMed Scopus (456) Google Scholar, 38Swain P.K. Chen S. Wang Q.-L. Affatigato L.M. Coats C.L. Brady K.D. Fishman G.A. Jacobson S.G. Swaroop A. Stone E. Sieving P.A. Zack D.J. Neuron. 1997; 19: 1329-1336Abstract Full Text Full Text PDF PubMed Scopus (222) Google Scholar, 39Freund C.L. Wang Q.-L. Chen S. Muskat B.L. Wiles C.D. Sheffield V.C. Jacobson S.G. McInnes R.R Zack D.J. Stone E.M. Nat. Genet. 1998; 18: 311-312Crossref PubMed Scopus (253) Google Scholar, 40Swaroop A. Wang Q.L. Wu W. Cook J. Coats C. Xu S. Chen S. Zack D.J. Sieving P.A. Hum. Mol. Genet. 1999; 8: 299-305Crossref PubMed Scopus (156) Google Scholar, 41Bessant D.A.R. Payne A.M. Mitton K.P. Wang Q.-L. Swain P.K. Plant C. Bird A.C. Zack D.J. Swaroop A. Bhattacharya S.S. Nat. Genet. 1999; 21: 355-356Crossref PubMed Scopus (152) Google Scholar). Based on these findings, we hypothesized that the transcriptional synergy between NRL and CRX in rhodopsin regulation results from cooperativity in binding to adjacent NRE and Ret-4 (or BAT-1) sites and/or from direct physical interaction, leading to the formation of a stable enhanceosome and/or initiation complex. In this paper, we demonstrate direct interaction between the leucine zipper of NRL and the homeodomain of CRX using the yeast two-hybrid interaction trap andin vitro glutathione S-transferase (GST) pull-down assays. The two-hybrid screening in yeast was carried out according to the published procedure (42Golemis E.A. Gyuris J. Brent R. Ausubel F.M. Brent R. Kingston R.E. Moore D.D. Seidman J.G. Smith J.A. Struhl K. Current Protocols in Molecular Biology. Greene Publishing Associates, New York1996: 20.1.1-20.1.28Google Scholar), with minor modifications. The bait construct was generated by cloning the SacII–PpuMI fragment of the human NRL cDNA (29Swaroop A. Xu J. Pawar H. Jackson A. Skolnick C. Agarwal N. Proc. Natl. Acad. Sci. U. S. A. 1992; 89: 266-270Crossref PubMed Scopus (269) Google Scholar) at the PvuII site of the pHybLex/Zeo vector (Invitrogen, Carlsbad, CA). The resulting construct, called pLex-NRL-ZIP, encoded the NRL leucine zipper (NRL-ZIP; amino acids 171–231) fused in frame with the LexA protein and did not display autologous activation of the reporter gene lacZ orHIS3 upon transformation in the yeast L40 strain (MATa his3Δ200 trp1–901 leu2–3112 ade2 LYS2::(4lexAop-HIS3) URA3::(8lexAop-lacZ) GAL4). The following constructs were obtained as part of the “Hybrid Hunter System” (from Invitrogen) and used for positive and negative interaction control experiments: pHybLex/Zeo-Fos2, pHybLex/Zeo-Laminin, and pYESTrp-Jun, which expressed the c-Fos leucine-zipper domain fused to LexA, laminin fused to LexA, and the c-Jun leucine zipper fused to the B42-activation domain (B42-AD), respectively. A bovine retina cDNA library (43Tai A.W. Chuang J.-Z. Bode C. Wolfrum U. Sung C.-H. Cell. 1999; 97: 877-887Abstract Full Text Full Text PDF PubMed Scopus (402) Google Scholar) in pACTII (prey vector with Gal4-activation domain) (a generous gift of Dr. C. H. Sung) was used to isolate interacting clones. Yeast strain L40 was sequentially transformed with pLex-NRL-ZIP and then with 10 μg of DNA from the retina library or purified clones in the prey vector, essentially as described (44Gietz D. Jean A.S. Woods R.A. Shiestl R.H. Nucleic Acids Res. 1992; 20: 1425Crossref PubMed Scopus (2899) Google Scholar). Double transformants were selected for presence of the bait and the prey vectors, and possible interactors were selected by growth on the appropriate yeast minimal medium (250 μm zeocin, minus Leu His) and by filter lift assay of β-galactosidase activity. Mixed bait and prey construct DNAs were recovered from double-positive yeast transformants after Zymolase-20T/SDS treatment (ICN Biomedicals, Aurora, OH). The pACTII retina library clones were separated from the bait vector DNA by electroporation into Escherichia coli XL1-Blue bacteria and growth on LB-ampicillin plates. Plasmid DNA, prepared by the alkaline lysis method, was used for sequencing and to test for false positives by retransformation of L40 yeast strains containing pHybLex/Zeo-Laminin and pLex-NRL-ZIP bait vectors. NRL cDNA fragments corresponding to the full-length protein of 237 amino acids and a truncated protein with the bZIP domain (amino acids 110–237; ΔNRL) were cloned in frame with GST in the pGex2TK vector (Amersham Pharmacia Biotech). The bovine CRX (bCRX) cDNA was cloned in pcDNA3.1/HisC mammalian expression vector (Invitrogen, Carlsbad, CA). A series of N- and C-terminal deletions were generated by polymerase chain reaction amplification usingPfu DNA polymerase (Strategene, La Jolla, CA), with the wild-type bCRX and primers corresponding to the appropriate end sequences with added BamHI (5′) or EcoRI (3′) site (see Fig. 5 A). The resulting polymerase chain reaction fragments were digested with BamHI and EcoRI, gel-purified, and subcloned intoBamHI/EcoRI-digested pcDNA 3.1/HisC vector. All deletions are fused in frame with the His6 tag at the N terminus and contain a stop codon at the C terminus. The sequence of each deletion construct was confirmed using a Perkin-Elmer ABI Prism DNA sequencing kit and ABI Prism 310 Genetic Analyzer. The GST, GST-NRL, and GSTΔNRL proteins were produced in E. coli strain BL21, essentially as described (45Artandi S. Calame K. Methods Mol. Genet. 1993; 1: 267-278Google Scholar). Briefly, the transformed bacteria were grown at 37 °C for 4 h (A600∼0.8) and induced with 0.5 mmisopropyl-thio-β-d-galactopyranoside for 3 h at 27 °C. Cells were disrupted by sonication in the lysis buffer (20 mm Tris-Cl, pH 8.0, 150 mm NaCl, 1 mm EDTA, and 1× Complete protease inhibitor mixture (Amersham Pharmacia Biotech)). E. coli-expressed proteins were purified using glutathione-Sepharose beads, as suggested by the manufacturer (Amersham Pharmacia Biotech). Protein concentrations were estimated using bicinchoninic acid reagent (Sigma). The GST-CRX-HD fusion protein was expressed and purified from E. coli BL21, as described previously (31Chen S. Wang Q. Nie Z. Sun H. Lennon G. Copeland N.G. Gilbert J. Jenkins N.A. Zack D.J. Neuron. 1997; 19: 1017-1030Abstract Full Text Full Text PDF PubMed Scopus (577) Google Scholar). bCRX constructs in pcDNA3.1/HisC vector (0.3 μg of double-stranded DNA) were translated in a 25-μl reaction in the presence of [35S]methionine (>1000 μCi/mmol; Amersham Pharmacia Biotech) using the T7-TNT Quick Coupled Transcription/Translation SystemTM (Promega). For in vitro interaction experiments, 7 μl of the 35S-labeled protein was incubated with glutathione-Sepharose-bound GST, GST-NRL, or GSTΔNRL protein (≥100 μg) in the binding buffer (20 mm Tris-Cl, pH 8.0, 150 mm NaCl, 0.2% Nonidet P-40). After 18 h of incubation at 4 °C on a nutator, the beads were washed five times in buffer containing 20 mm Tris-Cl, pH 8.0, 150 mm NaCl and, 0.2% Triton X-100. After the final wash, glutathione-Sepharose-bound proteins were resuspended in 60 μl of 2× SDS sample buffer, boiled for 5 min, and separated by SDS-polyacrylamide gel electrophoresis. To estimate the amount of labeled protein used in binding, 20% of the in vitrotranslated products were also examined on a parallel gel. The radiolabeled proteins were visualized by fluorography after treatment of the gel with AmplifyTM (Amersham Pharmacia Biotech), as described (46Ausubel F.M. Brent R. Kingston R.E. Moore D.D. Seidman J.G. Smith J.A. Struhl K. Current Protocols in Molecular Biology. John Wiley & Sons, Inc., New York1995Google Scholar). For in vitro translation studies, the NRL cDNA (29Swaroop A. Xu J. Pawar H. Jackson A. Skolnick C. Agarwal N. Proc. Natl. Acad. Sci. U. S. A. 1992; 89: 266-270Crossref PubMed Scopus (269) Google Scholar) was subcloned in pcDNA3.1 (Invitrogen). The plasmid construct was linearized by different restriction enzymes that digest at a unique site, and 35S-labeled full-length or truncated NRL proteins were produced using the T7-TNT Quick Coupled Transcription/Translation SystemTM (Promega). As an initial approach to evaluate the possibility of direct interaction between NRL and CRX, the yeast two-hybrid method was employed. We generated a bait vector that produced a fusion protein with LexA and the leucine zipper domain of NRL (NRL-ZIP). This bait construct did not autoactivate the HIS3 and lacZ reporter genes upon transformation in L40 yeast. The NRL-ZIP bait was used for screening a bovine retinal cDNA library in the pACTII vector (43Tai A.W. Chuang J.-Z. Bode C. Wolfrum U. Sung C.-H. Cell. 1999; 97: 877-887Abstract Full Text Full Text PDF PubMed Scopus (402) Google Scholar). Twenty-eight yeast double transformants that displayed fast growth on minus His medium were selected for further analysis. Filter lift tests of these His+ clones identified 26 clones that expressed β-galactosidase activity as well. Sequence analysis of the double positive clones revealed that 21 of them had bCRX sequence fused in frame to the Gal-4 activation domain. These clones could be divided into five subsets; four subsets included the 5′ noncoding sequence of bCRX, whereas one began at codon 14 of bCRX (Fig.2). All of the bCRX clones obtained from the screen contained the homeodomain. These clones, with or without the additional sequence from the 5′-untranslated region, were retransformed into L40 yeast. The presence of both the bait vector pLex-NRL-ZIP and bCRX-prey clones was found to be essential for growth on minus His medium (Fig. 3).Figure 3NRL-leucine zipper domain interacts with CRX in the yeast two-hybrid assay. A, the pACTII prey clones obtained from the screening of a bovine retina library were transformed into yeast L40 with and without the bait vector pLex-NRL-ZIP and replica-streaked onto minimal medium (minus Leu His, plus 10 mm aminotriazole) or minimal medium (minus Leu His, plus 250 μm zeocin, 10 mm aminotriazole). Three examples are shown here: Gal4-AD/CRX fusions without the 5′ noncoding sequence (clone CRX-N14), with the 5′ noncoding sequence (clone CRX-A), and a novel clone (KM1698–1). Only L40 double transformants with the bait vector grew on minus His plates.B, interaction of CRX is specific for the NRL-leucine zipper domain. Bait yeast strains L40/pHybLex/Zeo-Laminin and L40/pLex-NRL-ZIP were transformed with the CRX-N14 DNA, and double transformants were streaked onto minimal medium (minus Leu, minus His, plus 250 μm zeocin and 50 mm aminotriazole). The yeast transformants with the laminin bait and CRX prey did not grow, whereas NRL-ZIP and CRX double transformants activated the HIS3reporter gene and grew well. C, LexA-c-Fos (pHybLex/Zeo-Fos2) interacts with B42-AD-c-Jun (pYESTrp-Jun) in L40 yeast under identical assay conditions, whereas double transformants of LexA-laminin (pHybLex/Zeo-Laminin) with B42-AD-c-Jun did not show any interaction. Double transformants with c-Jun/c-Fos grew well on minimal medium (minus Trp, minus His, plus 250 μm zeocin and 50 mm aminotriazole), while those with laminin/c-Fos did not.View Large Image Figure ViewerDownload Hi-res image Download (PPT) Specificity of interaction between NRL-ZIP and bCRX was confirmed by transformation of pACTII-bCRX(N14) with pLex-NRL-ZIP or pHybLex/Zeo-Laminin and streaking the double transformants onto minus His plates containing 50 mm aminotriazole. As shown in Fig. 3 B, only yeast double transformants with pLex-NRL-ZIP grew on minus His plates, while the presence of the laminin bait construct did not result in the activation of the HIS3reporter gene. Under identical assay conditions, double transformants of L40 yeast with the c-Fos (pHybLex/Zeo-Fos2) bait and c-Jun (pYESTrp-Jun) prey constructs showed excellent growth on minus His plates, whereas the double transformants with the laminin bait (pHybLex/Zeo-Laminin) and c-Jun prey did not (Fig. 3 C). To confirm the direct interaction of NRL and CRX, we employed a pull-down assay with GST fusion proteins. Both GST-NRL (expressing GST fused with the full-length NRL protein) and GSTΔNRL (GST fused with the C-terminal 117 amino acids of NRL; primarily the bZIP domain), but not GST alone, were able to interact with the in vitrotranslated full-length CRX protein (Fig.4). This was consistent with the yeast two-hybrid screening, where NRL-ZIP bait was used. The addition of micrococcal nuclease to the GST pull-down reactions (in order to remove the DNA template) did not significantly alter the interaction of CRX with NRL (data not shown). To further define the region of bCRX protein that interacts with NRL, various deletion constructs (shown in Fig.5 A) were used for in vitro translation. In the pull-down assay, full-length (construct bCRX) and truncated proteins containing the HD and the glutamine-rich plus basic region (constructs N34, C160, and C208) demonstrated strong binding to GSTΔNRL (Fig. 5 B). Deletion of the N-terminal 87 residues (construct N88), which include most of the homeodomain, considerably reduced the binding of bCRX to GSTΔNRL, whereas the removal of the N-terminal 111 residues (construct N112, which also removes the Gln region) completely eliminated the binding (Fig.5 B). Deletion of the first 33 amino acids in bCRX (construct N34) did not have any effect on the interaction. Truncated bCRX proteins, which included the homeodomain (constructs HD and C107), consistently showed binding to NRL, although at a reduced level (Fig.5 B). SDS-PAGE analysis of the in vitro translated bCRX proteins revealed a comparable intensity of the labeled protein in binding reactions (Fig. 5 C). Taken together, the data strongly suggest that amino acids 34–88 (i.e. the homeodomain) constitute a region of CRX that is important for interaction with NRL-bZIP. Nonetheless, the binding efficiency was enhanced dramatically when the glutamine-rich plus basic region that follows the HD was included. Together with the yeast two-hybrid experiments, these results suggest that CRX-HD provides the primary interface for physical interaction with the leucine zipper motif of NRL. To define the domain of NRL responsible for interaction with the CRX homeodomain, GST-CRX-HD fusion protein was used for GST pull-down assay of in vitro translated NRL proteins. Full-length NRL and the NRL proteins with C-terminal deletions after amino acids 210 and 190 (NRL210 and NRL190) were prepared by in vitro translation. Full-length NRL displayed strong interaction with GST-CRX-HD by pull-down assay (Fig.6). Removal of the leucine zipper domain (either in part or most of it, constructs NRL210 and NRL190) decreased NRL's binding to CRX-HD (Fig. 6) and its ability to form a homodimer (data not shown). To further ascertain the importance of HD in NRL-CRX interaction, two mutations (R41W and R90W, identified in autosomal dominant cone-rod dystrophy (38Swain P.K. Chen S. Wang Q.-L. Affatigato L.M. Coats C.L. Brady K.D. Fishman G.A. Jacobson S.G. Swaroop A. Stone E. Sieving P.A. Zack D.J. Neuron. 1997; 19: 1329-1336Abstract Full Text Full Text PDF PubMed Scopus (222) Google Scholar) and Leber congenital amaurosis (40Swaroop A. Wang Q.L. Wu W. Cook J. Coats C. Xu S. Chen S. Zack D.J. Sieving P.A. Hum. Mol. Genet. 1999; 8: 299-305Crossref PubMed Scopus (156) Google Scholar), respectively) were incorporated in the CRX expression construct. The mutant CRX proteins, synthesized in vitro, were then used for the pull-down assay with GSTΔNRL. Both HD mutations, R41W and R90W, resulted in reduced binding to NRL-bZIP (Fig.7), consistent with the role of HD as the primary interface for interaction. Using two independent methods, we have provided evidence for direct physical interaction of NRL and CRX, two transcription factors implicated in rhodopsin regulation. The yeast two-hybrid studies show that the interaction is stable and functional within a cellular environment, whereas the GST pull-down experiments demonstrate direct association in vitro. These studies also indicate that the leucine zipper domain of NRL is sufficient for binding to CRX. Yeast two-hybrid experiments with LexA-bait fusions comprising the N-terminal or C-terminal halves of NRL-ZIP also showed interaction with the CRX prey (data not shown), suggesting a broad surface of contacts along the leucine zipper. Interestingly, the x-ray crystal structure of the interaction of the leucine zipper dimer AP1 (c-Fos/c-Jun) with NFAT, a β-scaffold type DNA-binding domain protein, illustrates an interaction surface that extends along the length of the leucine zipper region (47Chen L. Glover J.N.M. Hogan P.G. Rao A. Harrison S.C. Nature. 1998; 392: 42-48Crossref PubMed Scopus (414) Google Scholar). The bZIP transcription factors have been shown to interact with several other protein domains, and such interaction can either activate or repress expression of downstream target genes (48Yang-Yen H.-F. Chambard J.-C. Sun Y.-L. Smeal T. Schmidt T.J. Drouin J. Karin M. Cell. 1990; 62: 1205-1215Abstract Full Text PDF PubMed Scopus (1319) Google Scholar, 49Gavva N.R. Gavva R. Ermekova K. Sudol M. Shen C.-K.J. J. Biol. Chem. 1997; 272: 24105-24108Abstract Full Text Full Text PDF PubMed Scopus (60) Google Scholar, 50Yamaguchi Y. Wada T. Suzuki F. Takagi T. Hasegawa J. Handa H. Nucleic Acids Res. 1998; 26: 3854-3861Crossref PubMed Scopus (43) Google Scholar, 51Agarwal S.K. Siradanahalli C.G. Heppner C. Erdos M.R. Collins R.M. Park S.Y. Saggar S. Chandrasekharappa S.C. Collins F.S. Spiegel A.M. Marx S.J. Burns A.L. Cell. 1999; 96: 143-152Abstract Full Text Full Text PDF PubMed Scopus (533) Google Scholar). Our studies demonstrate that the bZIP motif is capable of interacting with the homeodomain as well. Although deletions can cause allosteric effects, the GST pull-down studies using CRX-HD with NRL deletion constructs (see Fig. 6) are consistent with this conclusion. While the leucine zipper region is sufficient for interaction with CRX (and specifically CRX-HD), it is probably not the only NRL domain that can interact with CRX. Other regions in NRL might also provide additional contact surfaces for interaction (see Fig.6). 2K. P. Mitton and A. Swaroop, unpublished data. During the yeast two-hybrid screening, we did not identify any leucine zipper proteins. This was surprising, since leucine zippers are shown to heterodimerize with other leucine zipper proteins. It is possible that CRX is the predominant interacting protein, and we did not exhaustively screen the prey library. Nonetheless, we think that the lack of leucine zipper proteins is due to the design of the bait construct. LexA binds to DNA as a homodimer through its N-terminal domain, while dimerization is mediated by its C-terminal domain (52Schnarr M. Oertel-Buchheit P. Kazamaier M. Granger-Schnarr M. Biochemie. 1991; 73: 423-431Crossref PubMed Scopus (91) Google Scholar). The use of NRL-ZIP as LexA fusion bait presents two possible outcomes. If the NRL-ZIP does not homodimerize, then the bait domain will be free to interact and form other potential leucine zipper associations with prey proteins from the retina library. This is illustrated by the fact that LexA-c-Fos bait fusion is free to interact with the B42-AD-c-Jun fusion protein, since the c-Fos leucine zipper does not homodimerize. On the other hand, if the NRL-ZIP forms a stable homodimer, complemented by LexA dimerization, it will not be available to interact with other leucine zipper domains. Nevertheless, prey proteins that interact with the exterior surface of the NRL-ZIP dimer would be picked up in this yeast two-hybrid screening. Analysis of the NRL-ZIP domain sequence reveals that the NRL-NRL homodimer has six potential ionic interactions, which would favor dimerization of similar strength as Fos-Jun association. The strong interaction of NRL with itself in GST pull-down assays strongly supports this possibility (data not shown). The yeast two-hybrid screening results are consistent with the hypothesis that NRL-ZIP bait exists as a homodimer and that the surface with leucine residues in the zipper is unavailable for interaction. We suggest that bCRX interacts with the NRL-ZIP homodimer and that the NRL interaction surface is the outside surface of the dimer (positionsa and d around the helical wheel) (53Glover J.N.M. Harrison S.C. Nature. 1995; 373: 257-261Crossref PubMed Scopus (671) Google Scholar). Additional experiments will be necessary to test this hypothesis. Homeodomains bind to DNA, and this is true for CRX as well (31Chen S. Wang Q. Nie Z. Sun H. Lennon G. Copeland N.G. Gilbert J. Jenkins N.A. Zack D.J. Neuron. 1997; 19: 1017-1030Abstract Full Text Full Text PDF PubMed Scopus (577) Google Scholar, 38Swain P.K. Chen S. Wang Q.-L. Affatigato L.M. Coats C.L. Brady K.D. Fishman G.A. Jacobson S.G. Swaroop A. Stone E. Sieving P.A. Zack D.J. Neuron. 1997; 19: 1329-1336Abstract Full Text Full Text PDF PubMed Scopus (222) Google Scholar,40Swaroop A. Wang Q.L. Wu W. Cook J. Coats C. Xu S. Chen S. Zack D.J. Sieving P.A. Hum. Mol. Genet. 1999; 8: 299-305Crossref PubMed Scopus (156) Google Scholar). Based on the published structure of the paired homeodomain (54Wilson D.S. Guenther B. Desplan C. Kuriyan J. Cell. 1995; 82: 709-719Abstract Full Text PDF PubMed Scopus (305) Google Scholar), we hypothesize that CRX-HD has three helix motifs: h1, from residues 50–59; h2, residues 69–76; and h3, residues 80–89. Helix-3 should be important for making contacts with DNA. It would then appear that the helices h1 and h2 are more accessible for interaction with NRL, consistent with the yeast two-hybrid screening results and CRX deletion analysis in GST pull-down assays. Both h1 and h2 are rich in ionic and polar amino acids that can make salt-bridge and hydrogen bonds with the NRL interaction surface. The conclusion that CRX-HD is involved in both DNA binding and protein-protein interaction is strengthened by the GST pull-down experiments with mutant CRX proteins (see Fig. 7). The glutamine-rich plus basic region that follows the homeodomain could provide a surface for additional interaction, since it enhanced the binding in pull-down assays. Homeodomain proteins appear to have a broad influence on gene expression, since they bind to a wide range of DNA sequences with similar affinity and this binding can be positively correlated to transcriptional activity (55Desplan C. Theis J. O'Farrell P.H. Cell. 1988; 54: 1081-1090Abstract Full Text PDF PubMed Scopus (337) Google Scholar, 56Carr A. Biggin M.D. EMBO J. 1999; 18: 1598-1608Crossref PubMed Scopus (76) Google Scholar). CRX may have a similar global influence on photoreceptor-specific gene expression, since it can bind to and transactivate from regulatory elements in several photoreceptor-specific genes, including rhodopsin, interphotoreceptor retinoid-binding protein, arrestin, and β-phosphodiesterase (31Chen S. Wang Q. Nie Z. Sun H. Lennon G. Copeland N.G. Gilbert J. Jenkins N.A. Zack D.J. Neuron. 1997; 19: 1017-1030Abstract Full Text Full Text PDF PubMed Scopus (577) Google Scholar, 57Livesey F.J. Furukawa T. Steffen M.A. Church G.M. Cepko C.L. Curr. Biol. 2000; 10: 301-310Abstract Full Text Full Text PDF PubMed Scopus (236) Google Scholar). In contrast, nonhomeodomain transcription factors bind to promoters in fewer genes with greater sequence specificity (58Bulger M. Groudine M. Genes Dev. 1999; 13: 2465-2477Crossref PubMed Scopus (375) Google Scholar). Such transcription factors, like NRL, may influence the specificity of gene transcription while homeodomain proteins, such as CRX, may facilitate structural control of larger chromatin regions and help facilitate the effects of other transcription factors upon the enhanceosome (56Carr A. Biggin M.D. EMBO J. 1999; 18: 1598-1608Crossref PubMed Scopus (76) Google Scholar). Interaction of NRL with CRX in the two test systems (yeast two-hybrid and GST pull-down) did not require the presence of RPPR sequence elements. The addition of micrococcal nuclease to the GST pull-down reactions (in order to remove DNA template) did not significantly alter the interaction of CRX with NRL. This is different from the interaction of AP1 and NFAT, which occurs only when their cognate DNA binding region is present to form a quarternary complex (47Chen L. Glover J.N.M. Hogan P.G. Rao A. Harrison S.C. Nature. 1998; 392: 42-48Crossref PubMed Scopus (414) Google Scholar, 59Jain J. McCaffrey P.G. Miner Z. Kerppola T.K. Lambert J.N. Verdine G.L. Curran T. Rao A. Nature. 1993; 365: 352-355Crossref PubMed Scopus (681) Google Scholar). Our studies raise the possibility that the two activator proteins (NRL and CRX) form a stable complex by directly interacting with each other and probably with other proteins prior to their binding to their cognate cis-sequence elements in RPPR. CRX is shown to bind to Ret-4, BAT-1, and Ret1 elements in RPPR in vitro (31Chen S. Wang Q. Nie Z. Sun H. Lennon G. Copeland N.G. Gilbert J. Jenkins N.A. Zack D.J. Neuron. 1997; 19: 1017-1030Abstract Full Text Full Text PDF PubMed Scopus (577) Google Scholar). We hypothesize that a stable NRL-CRX complex would influence the recognition of Ret-4 and BAT-1 flanking the NRE and provide binding sequence specificity during the organization of the rhodopsin enhanceosome. In addition, this interaction may result in cooperative and efficient DNA binding and explain their synergistic transactivation of the rhodopsin promoter. Mobility shift and DNase I experiments using the NRL and CRX proteins may help to confirm if the distribution of CRX on RPPR is influenced by NRL. The ability of NRL and CRX to physically interact with each other correlates well with their functional synergistic interaction at the rhodopsin promoter and illustrates one possible mechanism of context-dependent transcriptional regulation (60Fry C.J. Farnham P.J. J. Biol. Chem. 1999; 274: 29583-29586Abstract Full Text Full Text PDF PubMed Scopus (137) Google Scholar). This interaction also points to cross-talk among different signal transduction pathways that activate or modulate rhodopsin expression in developing and mature photoreceptors. Further investigations are in progress to identify and characterize other activator proteins that are also involved in the formation of the rhodopsin enhanceosome. Elucidation of extracellular factors that influence the expression and activity of NRL, CRX, and these other factors should provide important new insights into the regulation of rhodopsin expression. We are grateful to Dr. Ching-Hwa Sung (Dyson Vision Research Institute, Cornell University Medical College, New York) for providing the bovine retina cDNA library in pACTII vector. We thank Drs. R. Daniel Gietz and Anna Evans for constructive advice and discussions and thank Sharyn Ferrara for assistance in preparing the manuscript." @default.
• W2155015939 created "2016-06-24" @default.
• W2155015939 creator A5014783875 @default.
• W2155015939 creator A5040841311 @default.
• W2155015939 creator A5048831498 @default.
• W2155015939 creator A5050544784 @default.
• W2155015939 creator A5051438607 @default.
• W2155015939 creator A5073242437 @default.
• W2155015939 date "2000-09-01" @default.
• W2155015939 modified "2023-10-14" @default.
• W2155015939 title "The Leucine Zipper of NRL Interacts with the CRX Homeodomain" @default.
• W2155015939 cites W1507254050 @default.
• W2155015939 cites W1508022627 @default.
• W2155015939 cites W1512108330 @default.
• W2155015939 cites W1539101364 @default.
• W2155015939 cites W1589588578 @default.
• W2155015939 cites W1597109281 @default.
• W2155015939 cites W1632044028 @default.
• W2155015939 cites W1782414624 @default.
• W2155015939 cites W1847275787 @default.
• W2155015939 cites W1964064521 @default.
• W2155015939 cites W1969770714 @default.
• W2155015939 cites W1973912514 @default.
• W2155015939 cites W1983880463 @default.
• W2155015939 cites W1987557547 @default.
• W2155015939 cites W1991185690 @default.
• W2155015939 cites W2006295023 @default.
• W2155015939 cites W2009716064 @default.
• W2155015939 cites W2016774526 @default.
• W2155015939 cites W2017035463 @default.
• W2155015939 cites W2022987966 @default.
• W2155015939 cites W2026181894 @default.
• W2155015939 cites W2027066480 @default.
• W2155015939 cites W2031880789 @default.
• W2155015939 cites W2034527757 @default.
• W2155015939 cites W2036681136 @default.
• W2155015939 cites W2039441672 @default.
• W2155015939 cites W2041513882 @default.
• W2155015939 cites W2042963179 @default.
• W2155015939 cites W2043372546 @default.
• W2155015939 cites W2043789254 @default.
• W2155015939 cites W2046694802 @default.
• W2155015939 cites W2047893240 @default.
• W2155015939 cites W2048243902 @default.
• W2155015939 cites W2058224979 @default.
• W2155015939 cites W2059344357 @default.
• W2155015939 cites W2059773867 @default.
• W2155015939 cites W2066556283 @default.
• W2155015939 cites W2069036919 @default.
• W2155015939 cites W2072144082 @default.
• W2155015939 cites W2072414883 @default.
• W2155015939 cites W2072457100 @default.
• W2155015939 cites W2074366705 @default.
• W2155015939 cites W2077534857 @default.
• W2155015939 cites W2077909999 @default.
• W2155015939 cites W2083354279 @default.
• W2155015939 cites W2084282993 @default.
• W2155015939 cites W2086660631 @default.
• W2155015939 cites W2087950896 @default.
• W2155015939 cites W2099960599 @default.
• W2155015939 cites W2112921335 @default.
• W2155015939 cites W2118200380 @default.
• W2155015939 cites W2143713098 @default.
• W2155015939 cites W2171081956 @default.
• W2155015939 cites W2171442811 @default.
• W2155015939 cites W2205162546 @default.
• W2155015939 doi "https://doi.org/10.1074/jbc.m003658200" @default.
• W2155015939 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10887186" @default.
• W2155015939 hasPublicationYear "2000" @default.
• W2155015939 type Work @default.
• W2155015939 sameAs 2155015939 @default.
• W2155015939 citedByCount "197" @default.
• W2155015939 countsByYear W21550159392012 @default.
• W2155015939 countsByYear W21550159392013 @default.
• W2155015939 countsByYear W21550159392014 @default.
• W2155015939 countsByYear W21550159392015 @default.
• W2155015939 countsByYear W21550159392016 @default.
• W2155015939 countsByYear W21550159392017 @default.
• W2155015939 countsByYear W21550159392018 @default.
• W2155015939 countsByYear W21550159392019 @default.
• W2155015939 countsByYear W21550159392020 @default.
• W2155015939 countsByYear W21550159392021 @default.
• W2155015939 countsByYear W21550159392022 @default.
• W2155015939 countsByYear W21550159392023 @default.
• W2155015939 crossrefType "journal-article" @default.
• W2155015939 hasAuthorship W2155015939A5014783875 @default.
• W2155015939 hasAuthorship W2155015939A5040841311 @default.
• W2155015939 hasAuthorship W2155015939A5048831498 @default.
• W2155015939 hasAuthorship W2155015939A5050544784 @default.
• W2155015939 hasAuthorship W2155015939A5051438607 @default.
• W2155015939 hasAuthorship W2155015939A5073242437 @default.
• W2155015939 hasBestOaLocation W21550159391 @default.
• W2155015939 hasConcept C104317684 @default.
• W2155015939 hasConcept C105782903 @default.
• W2155015939 hasConcept C11413529 @default.
• W2155015939 hasConcept C121587040 @default.
• W2155015939 hasConcept C156860981 @default.
• W2155015939 hasConcept C185592680 @default.

• next