FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155016977> ?p ?o ?g. }

• W2155016977 abstract "Oncolytic virotherapy is a novel approach for the treatment of glioblastoma multiforme (GBM) which is still a fatal disease. Pathologic features of GBM are characterized by the infiltration with microglia/macrophages and a strong interaction between immune- and glioma cells. The aim of this study was to determine the role of microglia and astrocytes for oncolytic vaccinia virus (VACV) therapy of GBM.VACV LIVP 1.1.1 replication in C57BL/6 and Foxn1(nu/nu) mice with and without GL261 gliomas was analyzed. Furthermore, immunohistochemical analysis of microglia and astrocytes was investigated in non-, mock-, and LIVP 1.1.1-infected orthotopic GL261 gliomas in C57BL/6 mice. In cell culture studies virus replication and virus-mediated cell death of GL261 glioma cells was examined, as well as in BV-2 microglia and IMA2.1 astrocytes with M1 or M2 phenotypes. Co-culture experiments between BV-2 and GL261 cells and apoptosis/necrosis studies were performed. Organotypic slice cultures with implanted GL261 tumor spheres were used as additional cell culture system.We discovered that orthotopic GL261 gliomas upon intracranial virus delivery did not support replication of LIVP 1.1.1, similar to VACV-infected brains without gliomas. In addition, recruitment of Iba1(+) microglia and GFAP(+) astrocytes to orthotopically implanted GL261 glioma sites occurred already without virus injection. GL261 cells in culture showed high virus replication, while replication in BV-2 and IMA2.1 cells was barely detectable. The reduced viral replication in BV-2 cells might be due to rapid VACV-induced apoptotic cell death. In BV-2 and IMA 2.1 cells with M1 phenotype a further reduction of virus progeny and virus-mediated cell death was detected. Application of BV-2 microglial cells with M1 phenotype onto organotypic slice cultures with implanted GL261 gliomas resulted in reduced infection of BV-2 cells, whereas GL261 cells were well infected.Our results indicate that microglia and astrocytes, dependent on their activation state, may preferentially clear viral particles by immediate uptake after delivery. By acting as VACV traps they further reduce efficient virus infection of the tumor cells. These findings demonstrate that glia cells need to be taken into account for successful GBM therapy development." @default.
• W2155016977 created "2016-06-24" @default.
• W2155016977 creator A5003999618 @default.
• W2155016977 creator A5023470145 @default.
• W2155016977 creator A5051659038 @default.
• W2155016977 creator A5058307774 @default.
• W2155016977 creator A5073637434 @default.
• W2155016977 creator A5083248684 @default.
• W2155016977 date "2015-07-07" @default.
• W2155016977 modified "2023-09-25" @default.
• W2155016977 title "Microglia and astrocytes attenuate the replication of the oncolytic vaccinia virus LIVP 1.1.1 in murine GL261 gliomas by acting as vaccinia virus traps" @default.
• W2155016977 cites W15564899 @default.
• W2155016977 cites W1577874498 @default.
• W2155016977 cites W1593420214 @default.
• W2155016977 cites W1893588436 @default.
• W2155016977 cites W1957752652 @default.
• W2155016977 cites W1960031454 @default.
• W2155016977 cites W1968377494 @default.
• W2155016977 cites W1973224870 @default.
• W2155016977 cites W1980857287 @default.
• W2155016977 cites W1987015752 @default.
• W2155016977 cites W1994578631 @default.
• W2155016977 cites W2000614107 @default.
• W2155016977 cites W2005188784 @default.
• W2155016977 cites W2011361113 @default.
• W2155016977 cites W2011642035 @default.
• W2155016977 cites W2015929566 @default.
• W2155016977 cites W2016866438 @default.
• W2155016977 cites W2020452031 @default.
• W2155016977 cites W2024051895 @default.
• W2155016977 cites W2026524167 @default.
• W2155016977 cites W2027185351 @default.
• W2155016977 cites W2031671747 @default.
• W2155016977 cites W2033603857 @default.
• W2155016977 cites W2034387476 @default.
• W2155016977 cites W2036976739 @default.
• W2155016977 cites W2040773741 @default.
• W2155016977 cites W2042335926 @default.
• W2155016977 cites W2048898424 @default.
• W2155016977 cites W2055129010 @default.
• W2155016977 cites W2059677831 @default.
• W2155016977 cites W2059716804 @default.
• W2155016977 cites W2059782190 @default.
• W2155016977 cites W2061581934 @default.
• W2155016977 cites W2065360869 @default.
• W2155016977 cites W2067267561 @default.
• W2155016977 cites W2067438493 @default.
• W2155016977 cites W2068968368 @default.
• W2155016977 cites W2072225623 @default.
• W2155016977 cites W2078764481 @default.
• W2155016977 cites W2079054696 @default.
• W2155016977 cites W2084903397 @default.
• W2155016977 cites W2088308416 @default.
• W2155016977 cites W2093005409 @default.
• W2155016977 cites W2099108485 @default.
• W2155016977 cites W2100342970 @default.
• W2155016977 cites W2103934385 @default.
• W2155016977 cites W2106984937 @default.
• W2155016977 cites W2111354523 @default.
• W2155016977 cites W2111407090 @default.
• W2155016977 cites W2119742386 @default.
• W2155016977 cites W2121113421 @default.
• W2155016977 cites W2124667416 @default.
• W2155016977 cites W2128061865 @default.
• W2155016977 cites W2128810557 @default.
• W2155016977 cites W2139778190 @default.
• W2155016977 cites W2149040027 @default.
• W2155016977 cites W2150692877 @default.
• W2155016977 cites W2160382843 @default.
• W2155016977 cites W2161456562 @default.
• W2155016977 cites W2167017590 @default.
• W2155016977 cites W2172042148 @default.
• W2155016977 cites W2172216682 @default.
• W2155016977 cites W2202852041 @default.
• W2155016977 cites W2317583540 @default.
• W2155016977 cites W2412887786 @default.
• W2155016977 cites W2473243602 @default.
• W2155016977 cites W333643404 @default.
• W2155016977 cites W39814685 @default.
• W2155016977 cites W4211107382 @default.
• W2155016977 cites W4376596128 @default.
• W2155016977 doi "https://doi.org/10.1186/s12967-015-0586-x" @default.
• W2155016977 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4492094" @default.
• W2155016977 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26149494" @default.
• W2155016977 hasPublicationYear "2015" @default.
• W2155016977 type Work @default.
• W2155016977 sameAs 2155016977 @default.
• W2155016977 citedByCount "16" @default.
• W2155016977 countsByYear W21550169772016 @default.
• W2155016977 countsByYear W21550169772017 @default.
• W2155016977 countsByYear W21550169772018 @default.
• W2155016977 countsByYear W21550169772019 @default.
• W2155016977 countsByYear W21550169772020 @default.
• W2155016977 countsByYear W21550169772021 @default.
• W2155016977 countsByYear W21550169772022 @default.
• W2155016977 countsByYear W21550169772023 @default.
• W2155016977 crossrefType "journal-article" @default.
• W2155016977 hasAuthorship W2155016977A5003999618 @default.
• W2155016977 hasAuthorship W2155016977A5023470145 @default.
• W2155016977 hasAuthorship W2155016977A5051659038 @default.

• next