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- W2155110122 abstract "Abstract We describe a new method for separating and measuring urinary N-acetyl-beta-D-glucosaminidase isoenzymes by high-performance liquid chromatography. Isoenzymes are eluted from the anion-exchange column with a one-step linear gradient of NaCl solution. For continuous post-column quantification of the activities of these isoenzymes, we use an on-line post-column reactor and 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide as substrate; the methylumbelliferone formed in this reaction is quantified fluorimetrically. We discuss the effects of varying different components of the assay: NaCl concentration, the pH of the mobile phase and of the reaction reagent, substrate concentration, incubation temperature, and the geometry of the post-column reactor." @default.
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- W2155110122 date "1987-10-01" @default.
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- W2155110122 title "Rapid assay of N-acetyl-beta-D-glucosaminidase isoenzymes in urine by ion-exchange chromatography." @default.
- W2155110122 doi "https://doi.org/10.1093/clinchem/33.10.1796" @default.
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