FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155118062> ?p ?o ?g. }

• W2155118062 endingPage "2643" @default.
• W2155118062 startingPage "2628" @default.
• W2155118062 abstract "ABSTRACT We performed microarray analyses on RNA from human intestinal epithelial (HT-29) cells treated with the cytotoxic enterotoxin (Act) of Aeromonas hydrophila to examine global cellular transcriptional responses. Based on three independent experiments, Act upregulated the expression of 34 genes involved in cell growth, adhesion, signaling, immune responses (including interleukin-8 [IL-8] production), and apoptosis. We verified the upregulation of 14 genes by real-time reverse transcriptase-PCR and confirmed Act-induced production of IL-8 by enzyme-linked immunosorbent assay on supernatants from nonpolarized and polarized HT-29 cells. Maximal production of IL-8 in response to Act required the presence of intracellular calcium, since chelation of calcium with BAPTA-AM significantly reduced Act-induced IL-8 production in HT-29 cells. We also examined activation of mitogen-activated protein kinases and, as demonstrated by Western blot analysis of apical side-treated polarized HT-29 cells, Act induced phosphorylation of p38, c-Jun NH 2 -terminal kinase, and extracellular signal-regulated kinase 1/2. In addition, KinetWorks proteomics screening of whole-cell lysates revealed Act-induced phosphorylation of cyclic AMP-response element binding protein (CREB), c-Jun, adducin, protein kinase C, and signal transducer and activator of transcription 3 (STAT3) and decreased phosphorylation of protein kinase Bα, v-raf-1 murine leukemia viral oncogene homolog 1 (i.e., Raf1), and STAT1. We verified activation of CREB and activator protein 1 in polarized cells by gel shift assay. This is the first description of human intestinal epithelial cell transcriptional alterations, phosphorylation or activation of signaling molecules, cytokine production, and calcium mobilization in response to this toxin." @default.
• W2155118062 created "2016-06-24" @default.
• W2155118062 creator A5018398198 @default.
• W2155118062 creator A5022563446 @default.
• W2155118062 creator A5024831931 @default.
• W2155118062 creator A5039282448 @default.
• W2155118062 creator A5039961135 @default.
• W2155118062 creator A5041898039 @default.
• W2155118062 date "2005-05-01" @default.
• W2155118062 modified "2023-09-27" @default.
• W2155118062 title "Microarray and Proteomics Analyses of Human Intestinal Epithelial Cells Treated with the Aeromonas hydrophila Cytotoxic Enterotoxin" @default.
• W2155118062 cites W1562699269 @default.
• W2155118062 cites W1582652395 @default.
• W2155118062 cites W1594653092 @default.
• W2155118062 cites W1636221253 @default.
• W2155118062 cites W1639407471 @default.
• W2155118062 cites W1767727568 @default.
• W2155118062 cites W1800165852 @default.
• W2155118062 cites W1816690782 @default.
• W2155118062 cites W1873493101 @default.
• W2155118062 cites W1886472157 @default.
• W2155118062 cites W1965071570 @default.
• W2155118062 cites W1965211768 @default.
• W2155118062 cites W1971815270 @default.
• W2155118062 cites W1972085016 @default.
• W2155118062 cites W1973882490 @default.
• W2155118062 cites W1978137213 @default.
• W2155118062 cites W1983729225 @default.
• W2155118062 cites W1991783074 @default.
• W2155118062 cites W1995575464 @default.
• W2155118062 cites W1997490378 @default.
• W2155118062 cites W1999543220 @default.
• W2155118062 cites W2011089421 @default.
• W2155118062 cites W2011781801 @default.
• W2155118062 cites W2014181943 @default.
• W2155118062 cites W2016706952 @default.
• W2155118062 cites W2018348364 @default.
• W2155118062 cites W2024004416 @default.
• W2155118062 cites W2024390803 @default.
• W2155118062 cites W2027587367 @default.
• W2155118062 cites W2028647317 @default.
• W2155118062 cites W2030107112 @default.
• W2155118062 cites W2032730672 @default.
• W2155118062 cites W2033250797 @default.
• W2155118062 cites W2035008428 @default.
• W2155118062 cites W2036747248 @default.
• W2155118062 cites W2038923813 @default.
• W2155118062 cites W2039570072 @default.
• W2155118062 cites W2044057942 @default.
• W2155118062 cites W2047627595 @default.
• W2155118062 cites W2051395367 @default.
• W2155118062 cites W2059334396 @default.
• W2155118062 cites W2060947137 @default.
• W2155118062 cites W2064239099 @default.
• W2155118062 cites W2072349044 @default.
• W2155118062 cites W2073339066 @default.
• W2155118062 cites W2075325752 @default.
• W2155118062 cites W2080360077 @default.
• W2155118062 cites W2083630984 @default.
• W2155118062 cites W2084439623 @default.
• W2155118062 cites W2086614331 @default.
• W2155118062 cites W2088359631 @default.
• W2155118062 cites W2098384938 @default.
• W2155118062 cites W2099017217 @default.
• W2155118062 cites W2107277218 @default.
• W2155118062 cites W2108025070 @default.
• W2155118062 cites W2110174748 @default.
• W2155118062 cites W2110849397 @default.
• W2155118062 cites W2111590747 @default.
• W2155118062 cites W2120213729 @default.
• W2155118062 cites W2123671636 @default.
• W2155118062 cites W2130217058 @default.
• W2155118062 cites W2133762013 @default.
• W2155118062 cites W2135487116 @default.
• W2155118062 cites W2145133645 @default.
• W2155118062 cites W2158540015 @default.
• W2155118062 cites W2162977709 @default.
• W2155118062 cites W2163394733 @default.
• W2155118062 cites W2166809896 @default.
• W2155118062 cites W2184293178 @default.
• W2155118062 cites W2318332039 @default.
• W2155118062 cites W2424607300 @default.
• W2155118062 cites W4254533614 @default.
• W2155118062 cites W64593971 @default.
• W2155118062 cites W967464957 @default.
• W2155118062 cites W1964184380 @default.
• W2155118062 doi "https://doi.org/10.1128/iai.73.5.2628-2643.2005" @default.
• W2155118062 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1087361" @default.
• W2155118062 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15845465" @default.
• W2155118062 hasPublicationYear "2005" @default.
• W2155118062 type Work @default.
• W2155118062 sameAs 2155118062 @default.
• W2155118062 citedByCount "22" @default.
• W2155118062 countsByYear W21551180622012 @default.
• W2155118062 countsByYear W21551180622013 @default.
• W2155118062 countsByYear W21551180622014 @default.
• W2155118062 countsByYear W21551180622017 @default.
• W2155118062 countsByYear W21551180622019 @default.

• next