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• W2155123169 abstract "Surface gene mutants of hepatitis B virus (HBV) have been reported in a variety of patient groups. Because of limited data regarding these mutations in patients with occult HBV infections; we aimed to determine these mutations among high-risk patients with occult HBV infection. The presence of HBV-DNA was determined in patients with isolated anti-HBc by real-time polymerase chain reaction (PCR). Then, surface gene region was amplified by nested PCR and mutations were analyzed after sequencing. The mutations that resulted in nonfunctional hepatitis B surface antigen (HBsAg) were insertion of single nucleotide in 2 cases, which causes frameshift and single-nucleotide replacement, and premature stop codons at Leu15 and Gly10 in the other 2 cases. Amino acid substitution at amino acid position 207(S207N) was found in the other isolates. Our study suggested that “a” region mutations did not play a major role in HBsAg detection, and other genetic and nongenetic factors may be responsible for failure to detect HBsAg by routine laboratory tests." @default.
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• W2155123169 date "2010-03-01" @default.
• W2155123169 modified "2023-10-14" @default.
• W2155123169 title "Surface gene mutations of hepatitis B virus among high-risk patients with occult hepatitis B virus infection" @default.
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• W2155123169 doi "https://doi.org/10.1016/j.diagmicrobio.2009.10.006" @default.
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