FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155132395> ?p ?o ?g. }

• W2155132395 endingPage "8395" @default.
• W2155132395 startingPage "8384" @default.
• W2155132395 abstract "All plus-strand RNA viruses encode an RNA-dependent RNA polymerase (RdRp) that functions as the catalytic subunit of the viral replication/transcription complex, directing viral RNA synthesis in concert with other viral proteins and, sometimes, host proteins. RNA synthesis essentially can be initiated by two different mechanisms, de novo initiation and primer-dependent initiation. Most viral RdRps have been identified solely on the basis of comparative sequence analysis, and for many viruses the mechanism of initiation is unknown. In this study, using the family prototype equine arteritis virus (EAV), we address the mechanism of initiation of RNA synthesis in arteriviruses. The RdRp domains of the members of the arterivirus family, which are part of replicase subunit nsp9, were compared to coronavirus RdRps that belong to the same order of Nidovirales, as well as to other RdRps with known initiation mechanisms and three-dimensional structures. We report here the first successful expression and purification of an arterivirus RdRp that is catalytically active in the absence of other viral or cellular proteins. The EAV nsp9/RdRp initiates RNA synthesis by a de novo mechanism on homopolymeric templates in a template-specific manner. In addition, the requirements for initiation of RNA synthesis from the 3' end of the viral genome were studied in vivo using a reverse genetics approach. These studies suggest that the 3'-terminal nucleotides of the EAV genome play a critical role in viral RNA synthesis." @default.
• W2155132395 created "2016-06-24" @default.
• W2155132395 creator A5010959513 @default.
• W2155132395 creator A5035730795 @default.
• W2155132395 creator A5039783946 @default.
• W2155132395 creator A5067074504 @default.
• W2155132395 creator A5071436463 @default.
• W2155132395 creator A5079530459 @default.
• W2155132395 creator A5087144513 @default.
• W2155132395 date "2007-08-15" @default.
• W2155132395 modified "2023-10-14" @default.
• W2155132395 title "De Novo Initiation of RNA Synthesis by the Arterivirus RNA-Dependent RNA Polymerase" @default.
• W2155132395 cites W147358830 @default.
• W2155132395 cites W1507562456 @default.
• W2155132395 cites W1511151041 @default.
• W2155132395 cites W1519916614 @default.
• W2155132395 cites W1567952953 @default.
• W2155132395 cites W1902684255 @default.
• W2155132395 cites W1963879940 @default.
• W2155132395 cites W1965047713 @default.
• W2155132395 cites W1967154222 @default.
• W2155132395 cites W1971141327 @default.
• W2155132395 cites W1971168306 @default.
• W2155132395 cites W1971654966 @default.
• W2155132395 cites W1975366693 @default.
• W2155132395 cites W1980837534 @default.
• W2155132395 cites W1982090618 @default.
• W2155132395 cites W2000594526 @default.
• W2155132395 cites W2005610996 @default.
• W2155132395 cites W2011252618 @default.
• W2155132395 cites W2011374678 @default.
• W2155132395 cites W2017313617 @default.
• W2155132395 cites W2025071490 @default.
• W2155132395 cites W2025290907 @default.
• W2155132395 cites W2030140637 @default.
• W2155132395 cites W2034767282 @default.
• W2155132395 cites W2036930815 @default.
• W2155132395 cites W2042669556 @default.
• W2155132395 cites W2051514752 @default.
• W2155132395 cites W2054896136 @default.
• W2155132395 cites W2068740107 @default.
• W2155132395 cites W2071460423 @default.
• W2155132395 cites W2071968383 @default.
• W2155132395 cites W2073641163 @default.
• W2155132395 cites W2080725002 @default.
• W2155132395 cites W2084620659 @default.
• W2155132395 cites W2085323444 @default.
• W2155132395 cites W2092052419 @default.
• W2155132395 cites W2095425492 @default.
• W2155132395 cites W2097209034 @default.
• W2155132395 cites W2099159538 @default.
• W2155132395 cites W2102030104 @default.
• W2155132395 cites W2103854602 @default.
• W2155132395 cites W2105538224 @default.
• W2155132395 cites W2108615853 @default.
• W2155132395 cites W2108771986 @default.
• W2155132395 cites W2110658293 @default.
• W2155132395 cites W2110670926 @default.
• W2155132395 cites W2111166656 @default.
• W2155132395 cites W2113927019 @default.
• W2155132395 cites W2116184275 @default.
• W2155132395 cites W2119473061 @default.
• W2155132395 cites W2128967076 @default.
• W2155132395 cites W2133318050 @default.
• W2155132395 cites W2135745383 @default.
• W2155132395 cites W2138892620 @default.
• W2155132395 cites W2138957878 @default.
• W2155132395 cites W2141157874 @default.
• W2155132395 cites W2141349175 @default.
• W2155132395 cites W2142597062 @default.
• W2155132395 cites W2144881432 @default.
• W2155132395 cites W2145649504 @default.
• W2155132395 cites W2146202166 @default.
• W2155132395 cites W2147880851 @default.
• W2155132395 cites W2149160071 @default.
• W2155132395 cites W2149495938 @default.
• W2155132395 cites W2149824411 @default.
• W2155132395 cites W2151937581 @default.
• W2155132395 cites W2152560721 @default.
• W2155132395 cites W2154666681 @default.
• W2155132395 cites W2159869554 @default.
• W2155132395 cites W2162632699 @default.
• W2155132395 cites W2169621156 @default.
• W2155132395 cites W31270631 @default.
• W2155132395 doi "https://doi.org/10.1128/jvi.00564-07" @default.
• W2155132395 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1951334" @default.
• W2155132395 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17537850" @default.
• W2155132395 hasPublicationYear "2007" @default.
• W2155132395 type Work @default.
• W2155132395 sameAs 2155132395 @default.
• W2155132395 citedByCount "61" @default.
• W2155132395 countsByYear W21551323952012 @default.
• W2155132395 countsByYear W21551323952013 @default.
• W2155132395 countsByYear W21551323952014 @default.
• W2155132395 countsByYear W21551323952015 @default.
• W2155132395 countsByYear W21551323952016 @default.
• W2155132395 countsByYear W21551323952017 @default.
• W2155132395 countsByYear W21551323952018 @default.

• next