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- W2155151508 abstract "Clathrin-mediated endocytosis of synaptic vesicle membranes involves the recruitment of clathrin and AP-2 adaptor complexes to the presynaptic plasma membrane. Phosphoinositides have been implicated in nucleating coat assembly by directly binding to several endocytotic proteins including AP-2 and AP180. Here, we show that the stimulatory effect of ATP and GTPgammaS on clathrin coat recruitment is mediated at least in part by increased levels of PIP2. We also provide evidence for a role of ADP-ribosylation factor 6 (ARF6) via direct stimulation of a synaptically enriched phosphatidylinositol 4-phosphate 5-kinase type Igamma (PIPKIgamma), in this effect. These data suggest a model according to which activation of PIPKIgamma by ARF6-GTP facilitates clathrin-coated pit assembly at the synapse." @default.
- W2155151508 created "2016-06-24" @default.
- W2155151508 creator A5009134392 @default.
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- W2155151508 creator A5049447022 @default.
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- W2155151508 date "2003-07-07" @default.
- W2155151508 modified "2023-10-14" @default.
- W2155151508 title "ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type Iγ" @default.
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- W2155151508 doi "https://doi.org/10.1083/jcb.200301006" @default.
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