FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155157043> ?p ?o ?g. }

• W2155157043 endingPage "5288" @default.
• W2155157043 startingPage "5279" @default.
• W2155157043 abstract "ABSTRACT Transcriptional activators function in vivo via binding sites that may be packaged into chromatin. Here we show that whereas the transcriptional activator GAL4 is strongly able to perturb chromatin structure via a nucleosomal binding site in yeast, GCN4 does so poorly. Correspondingly, GCN4 requires assistance from an accessory protein, RAP1, for activation of the HIS4 promoter, whereas GAL4 does not. The requirement for RAP1 for GCN4-mediated HIS4 activation is dictated by the DNA-binding domain of GCN4 and not the activation domain, suggesting that RAP1 assists GCN4 in gaining access to its binding site. Consistent with this, overexpression of GCN4 partially alleviates the requirement for RAP1, whereas HIS4 activation via a weak GAL4 binding site requires RAP1. RAP1 is extremely effective at interfering with positioning of a nucleosome containing its binding site, consistent with a role in opening chromatin at the HIS4 promoter. Furthermore, increasing the spacing between binding sites for RAP1 and GCN4 by 5 or 10 bp does not impair HIS4 activation, indicating that cooperative protein-protein interactions are not involved in transcriptional facilitation by RAP1. We conclude that an important role of RAP1 is to assist activator binding by opening chromatin." @default.
• W2155157043 created "2016-06-24" @default.
• W2155157043 creator A5013323680 @default.
• W2155157043 creator A5035611482 @default.
• W2155157043 date "1999-08-01" @default.
• W2155157043 modified "2023-10-07" @default.
• W2155157043 title "Chromatin Opening and Transactivator Potentiation by RAP1 in Saccharomyces cerevisiae" @default.
• W2155157043 cites W15025769 @default.
• W2155157043 cites W1534277217 @default.
• W2155157043 cites W1555086548 @default.
• W2155157043 cites W1564217760 @default.
• W2155157043 cites W1615898285 @default.
• W2155157043 cites W1652729057 @default.
• W2155157043 cites W1756254534 @default.
• W2155157043 cites W1807079294 @default.
• W2155157043 cites W183549353 @default.
• W2155157043 cites W1886333092 @default.
• W2155157043 cites W1926397638 @default.
• W2155157043 cites W1942920824 @default.
• W2155157043 cites W1963685227 @default.
• W2155157043 cites W1964461239 @default.
• W2155157043 cites W1967390330 @default.
• W2155157043 cites W1970011172 @default.
• W2155157043 cites W1973148221 @default.
• W2155157043 cites W1977186059 @default.
• W2155157043 cites W1981765089 @default.
• W2155157043 cites W1984354326 @default.
• W2155157043 cites W1993374805 @default.
• W2155157043 cites W2007675804 @default.
• W2155157043 cites W2015617558 @default.
• W2155157043 cites W2018345118 @default.
• W2155157043 cites W2020377415 @default.
• W2155157043 cites W2028110618 @default.
• W2155157043 cites W2034892963 @default.
• W2155157043 cites W2041309113 @default.
• W2155157043 cites W2042543706 @default.
• W2155157043 cites W2043763592 @default.
• W2155157043 cites W2047076849 @default.
• W2155157043 cites W2052695592 @default.
• W2155157043 cites W2067863011 @default.
• W2155157043 cites W2070159701 @default.
• W2155157043 cites W2072548321 @default.
• W2155157043 cites W2075407612 @default.
• W2155157043 cites W2077351082 @default.
• W2155157043 cites W2078952292 @default.
• W2155157043 cites W2081062649 @default.
• W2155157043 cites W2087181226 @default.
• W2155157043 cites W2087640316 @default.
• W2155157043 cites W2091512138 @default.
• W2155157043 cites W2099061740 @default.
• W2155157043 cites W2101023417 @default.
• W2155157043 cites W2105487716 @default.
• W2155157043 cites W2120711292 @default.
• W2155157043 cites W2121722949 @default.
• W2155157043 cites W2127501286 @default.
• W2155157043 cites W2128168784 @default.
• W2155157043 cites W2129314778 @default.
• W2155157043 cites W2130208743 @default.
• W2155157043 cites W2132440338 @default.
• W2155157043 cites W2142643208 @default.
• W2155157043 cites W2155190447 @default.
• W2155157043 cites W2275861305 @default.
• W2155157043 cites W2344014880 @default.
• W2155157043 cites W2346724938 @default.
• W2155157043 cites W28564324 @default.
• W2155157043 cites W346149210 @default.
• W2155157043 cites W38671249 @default.
• W2155157043 cites W4211121711 @default.
• W2155157043 cites W4234411669 @default.
• W2155157043 cites W4244207162 @default.
• W2155157043 cites W4244288506 @default.
• W2155157043 doi "https://doi.org/10.1128/mcb.19.8.5279" @default.
• W2155157043 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/84371" @default.
• W2155157043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10409719" @default.
• W2155157043 hasPublicationYear "1999" @default.
• W2155157043 type Work @default.
• W2155157043 sameAs 2155157043 @default.
• W2155157043 citedByCount "102" @default.
• W2155157043 countsByYear W21551570432012 @default.
• W2155157043 countsByYear W21551570432013 @default.
• W2155157043 countsByYear W21551570432014 @default.
• W2155157043 countsByYear W21551570432015 @default.
• W2155157043 countsByYear W21551570432016 @default.
• W2155157043 countsByYear W21551570432017 @default.
• W2155157043 countsByYear W21551570432018 @default.
• W2155157043 countsByYear W21551570432019 @default.
• W2155157043 countsByYear W21551570432020 @default.
• W2155157043 countsByYear W21551570432021 @default.
• W2155157043 countsByYear W21551570432022 @default.
• W2155157043 countsByYear W21551570432023 @default.
• W2155157043 crossrefType "journal-article" @default.
• W2155157043 hasAuthorship W2155157043A5013323680 @default.
• W2155157043 hasAuthorship W2155157043A5035611482 @default.
• W2155157043 hasBestOaLocation W21551570432 @default.
• W2155157043 hasConcept C104317684 @default.
• W2155157043 hasConcept C107824862 @default.
• W2155157043 hasConcept C12554922 @default.
• W2155157043 hasConcept C25166345 @default.

• next