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- W2155163901 abstract "Cassava mosaic disease (CMD) is undoubtedly the most widespread disease in cassava (Manihot esculenta Crantz) in sub-Saharan Africa. While African cassava mosaic virus-Kenya (ACMV-KE), African cassava mosaic virus-Uganda (ACMV-UG), African cassava mosaic virus-India (ACMV-IC), East African cassava mosaic virus-Cameroon (EACMV-CM) and a recombinant East African cassava mosaic virus-India/Cameroon (EACMV-CM/IC) can be transmitted easily and repeatedly to Nicotiana benthamiana plants, difficulty in mechanical transmission and multiplication of EACMV-UG and South African cassava mosaic virus (SACMV) in N. benthamiana appears to place them biologically in a different group. In vitro grown 3-week-old cassava plants were biolistically inoculated with SACMV DNA A and B dimers and infectivity measured using a system based on visual assessment. We report for the first time successful infection, induced by SACMV DNA A and B dimer, of cassava cultivar TMS60444 using biolistic inoculation. Typical mosaic symptoms started to show at 14 days post-inoculation (dpi) in infected cassava plants, and SACMV replication was confirmed using PCR. Potential applications of biolistic infection of SACMV are also discussed." @default.
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- W2155163901 date "2006-01-16" @default.
- W2155163901 modified "2023-09-27" @default.
- W2155163901 title "Biolistic inoculation of cassava (Manihot esculenta Crantz) with South African cassava mosaic virus" @default.
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- W2155163901 doi "https://doi.org/10.5897/ajb2006.000-5009" @default.
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