FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155183644> ?p ?o ?g. }

• W2155183644 endingPage "2554" @default.
• W2155183644 startingPage "2543" @default.
• W2155183644 abstract "Resistance to the polycationic antibiotic polymyxin B and expression of the outer-membrane protein OprH in the opportunistic pathogen Pseudomonas aeruginosa both involve the PhoP-PhoQ two-component regulatory system. The genes for this system form an operon with oprH, oprH-phoP-phoQ, that responds to Mg(2+) starvation and PhoP levels. In this study, the Mg(2+)-regulated promoter for this operon was mapped upstream of oprH by primer-extension experiments. An oprH::xylE-Gm(R) mutant H855 was constructed and measurement of the catechol 2,3-dioxygenase activity expressed from this transcriptional fusion provided evidence for a second, weak promoter for phoP-phoQ. Wild-type P. aeruginosa PAO1 strain H103 was found to exhibit Mg(2+)-regulated resistance to the alpha-helical antimicrobial cationic peptide CP28 in addition to its previously characterized resistance to polymyxin B. Resistance to this peptide was unchanged in the OprH-null mutant H855 and a PhoP-null mutant H851. In contrast, PhoQ-null mutant H854 demonstrated constitutive CP28 resistance. Northern blot analysis revealed constitutive expression of phoP in this strain, implicating PhoP-PhoQ in the resistance of P. aeruginosa to cationic peptides. Furthermore, all three null-mutant strains demonstrated increased resistance to the aminoglycoside antibiotics streptomycin, kanamycin and amikacin. Two additional mutant strains, H895 and H896, were constructed that carried unmarked deletions in oprH and were found to exhibit aminoglycoside susceptibility equivalent to that of the wild-type. This result provided definitive evidence that OprH is not involved in P. aeruginosa aminoglycoside resistance and that the changes in resistance in strain H855 and a previously reported oprH mutant were due to polar effects on phoP-phoQ rather than loss of OprH expression. A role for PhoP-PhoQ in resistance to aminoglycosides is envisaged that is distinct from that in resistance to cationic peptides and polymyxin B." @default.
• W2155183644 created "2016-06-24" @default.
• W2155183644 creator A5003608962 @default.
• W2155183644 creator A5004359198 @default.
• W2155183644 creator A5066530115 @default.
• W2155183644 date "2000-10-01" @default.
• W2155183644 modified "2023-09-26" @default.
• W2155183644 title "Role of Pseudomonas aeruginosa PhoP-PhoQ in resistance to antimicrobial cationic peptides and aminoglycosides" @default.
• W2155183644 cites W1568314805 @default.
• W2155183644 cites W1615284855 @default.
• W2155183644 cites W1670573078 @default.
• W2155183644 cites W1675011399 @default.
• W2155183644 cites W1780502181 @default.
• W2155183644 cites W1785999156 @default.
• W2155183644 cites W1902470244 @default.
• W2155183644 cites W1958241309 @default.
• W2155183644 cites W1966350980 @default.
• W2155183644 cites W1993513102 @default.
• W2155183644 cites W1995426359 @default.
• W2155183644 cites W1999408727 @default.
• W2155183644 cites W2003112779 @default.
• W2155183644 cites W2027229485 @default.
• W2155183644 cites W2031703416 @default.
• W2155183644 cites W2056618449 @default.
• W2155183644 cites W2066520670 @default.
• W2155183644 cites W2073521181 @default.
• W2155183644 cites W2075893586 @default.
• W2155183644 cites W2081960029 @default.
• W2155183644 cites W2089086408 @default.
• W2155183644 cites W2090664858 @default.
• W2155183644 cites W2103145771 @default.
• W2155183644 cites W2105246389 @default.
• W2155183644 cites W2110924446 @default.
• W2155183644 cites W2111584103 @default.
• W2155183644 cites W2111667046 @default.
• W2155183644 cites W2113410040 @default.
• W2155183644 cites W2119889633 @default.
• W2155183644 cites W2151387894 @default.
• W2155183644 cites W2159337861 @default.
• W2155183644 cites W4238660447 @default.
• W2155183644 cites W4244732422 @default.
• W2155183644 doi "https://doi.org/10.1099/00221287-146-10-2543" @default.
• W2155183644 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11021929" @default.
• W2155183644 hasPublicationYear "2000" @default.
• W2155183644 type Work @default.
• W2155183644 sameAs 2155183644 @default.
• W2155183644 citedByCount "183" @default.
• W2155183644 countsByYear W21551836442012 @default.
• W2155183644 countsByYear W21551836442013 @default.
• W2155183644 countsByYear W21551836442014 @default.
• W2155183644 countsByYear W21551836442015 @default.
• W2155183644 countsByYear W21551836442016 @default.
• W2155183644 countsByYear W21551836442017 @default.
• W2155183644 countsByYear W21551836442018 @default.
• W2155183644 countsByYear W21551836442019 @default.
• W2155183644 countsByYear W21551836442020 @default.
• W2155183644 countsByYear W21551836442021 @default.
• W2155183644 countsByYear W21551836442022 @default.
• W2155183644 countsByYear W21551836442023 @default.
• W2155183644 crossrefType "journal-article" @default.
• W2155183644 hasAuthorship W2155183644A5003608962 @default.
• W2155183644 hasAuthorship W2155183644A5004359198 @default.
• W2155183644 hasAuthorship W2155183644A5066530115 @default.
• W2155183644 hasConcept C104317684 @default.
• W2155183644 hasConcept C143065580 @default.
• W2155183644 hasConcept C153911025 @default.
• W2155183644 hasConcept C203075996 @default.
• W2155183644 hasConcept C207583985 @default.
• W2155183644 hasConcept C2777637488 @default.
• W2155183644 hasConcept C2777787772 @default.
• W2155183644 hasConcept C2780026444 @default.
• W2155183644 hasConcept C501593827 @default.
• W2155183644 hasConcept C523546767 @default.
• W2155183644 hasConcept C54355233 @default.
• W2155183644 hasConcept C55493867 @default.
• W2155183644 hasConcept C86803240 @default.
• W2155183644 hasConcept C89423630 @default.
• W2155183644 hasConceptScore W2155183644C104317684 @default.
• W2155183644 hasConceptScore W2155183644C143065580 @default.
• W2155183644 hasConceptScore W2155183644C153911025 @default.
• W2155183644 hasConceptScore W2155183644C203075996 @default.
• W2155183644 hasConceptScore W2155183644C207583985 @default.
• W2155183644 hasConceptScore W2155183644C2777637488 @default.
• W2155183644 hasConceptScore W2155183644C2777787772 @default.
• W2155183644 hasConceptScore W2155183644C2780026444 @default.
• W2155183644 hasConceptScore W2155183644C501593827 @default.
• W2155183644 hasConceptScore W2155183644C523546767 @default.
• W2155183644 hasConceptScore W2155183644C54355233 @default.
• W2155183644 hasConceptScore W2155183644C55493867 @default.
• W2155183644 hasConceptScore W2155183644C86803240 @default.
• W2155183644 hasConceptScore W2155183644C89423630 @default.
• W2155183644 hasIssue "10" @default.
• W2155183644 hasLocation W21551836441 @default.
• W2155183644 hasLocation W21551836442 @default.
• W2155183644 hasOpenAccess W2155183644 @default.
• W2155183644 hasPrimaryLocation W21551836441 @default.
• W2155183644 hasRelatedWork W1481027913 @default.
• W2155183644 hasRelatedWork W1994780701 @default.

• next