FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155189368> ?p ?o ?g. }

• W2155189368 endingPage "70" @default.
• W2155189368 startingPage "63" @default.
• W2155189368 abstract "Rolling Circle Amplification (RCA) of DNA is a sensitive and cost effective method for the rapid identification of pathogenic fungi without the need for sequencing. Amplification products can be visualized on 1% agarose gel to verify the specificity of probe-template binding or directly by adding fluorescent dyes. Fusarium Head Blight (FHB) is currently the world's largest threat to the production of cereal crops with the production of a range of mycotoxins as an additional risk. We designed sets of RCA padlock probes based on polymorphisms in the elongation factor 1-α (EF-1α) gene to detect the dominant FHB species, comprising lineages of the Fusarium graminearum species complex (FGSC). The method also enabled the identification of species of the Fusarium oxysporum (FOSC), the Fusarium incarnatum–equiseti (FIESC), and the Fusarium tricinctum (FTSC) species complexes, and used strains from the CBS culture collection as reference. Subsequently probes were applied to characterize isolates from wheat and wild grasses, and inoculated wheat kernels. The RCA assays successfully amplified DNA of the target fungi, both in environmental samples and in the contaminated wheat samples, while no cross reactivity was observed with uncontaminated wheat or related Fusarium species. As RCA does not require expensive instrumentation, the technique has a good potential for local and point of care screening for toxigenic Fusarium species in cereals." @default.
• W2155189368 created "2016-06-24" @default.
• W2155189368 creator A5005346346 @default.
• W2155189368 creator A5018853050 @default.
• W2155189368 creator A5034144223 @default.
• W2155189368 creator A5048896394 @default.
• W2155189368 creator A5065656301 @default.
• W2155189368 creator A5072522026 @default.
• W2155189368 creator A5085601234 @default.
• W2155189368 date "2012-04-01" @default.
• W2155189368 modified "2023-10-03" @default.
• W2155189368 title "Rapid identification of Fusarium graminearum species complex using Rolling Circle Amplification (RCA)" @default.
• W2155189368 cites W1562049809 @default.
• W2155189368 cites W1748584362 @default.
• W2155189368 cites W1959886451 @default.
• W2155189368 cites W1963801963 @default.
• W2155189368 cites W1969246781 @default.
• W2155189368 cites W1985951348 @default.
• W2155189368 cites W1991786203 @default.
• W2155189368 cites W2001780576 @default.
• W2155189368 cites W2010974451 @default.
• W2155189368 cites W2020325178 @default.
• W2155189368 cites W2022224847 @default.
• W2155189368 cites W2024389084 @default.
• W2155189368 cites W2028789791 @default.
• W2155189368 cites W2038311101 @default.
• W2155189368 cites W2039250780 @default.
• W2155189368 cites W2051478751 @default.
• W2155189368 cites W2066789848 @default.
• W2155189368 cites W2088740571 @default.
• W2155189368 cites W2093003933 @default.
• W2155189368 cites W2105737227 @default.
• W2155189368 cites W2112041675 @default.
• W2155189368 cites W2112666939 @default.
• W2155189368 cites W2113972577 @default.
• W2155189368 cites W2117006050 @default.
• W2155189368 cites W2120981631 @default.
• W2155189368 cites W2123817175 @default.
• W2155189368 cites W2128329887 @default.
• W2155189368 cites W2128979353 @default.
• W2155189368 cites W2130834132 @default.
• W2155189368 cites W2138012030 @default.
• W2155189368 cites W2138176711 @default.
• W2155189368 cites W2146647617 @default.
• W2155189368 cites W2159256660 @default.
• W2155189368 cites W2161347058 @default.
• W2155189368 cites W2169212359 @default.
• W2155189368 cites W2170139494 @default.
• W2155189368 cites W2170218835 @default.
• W2155189368 cites W4255770413 @default.
• W2155189368 doi "https://doi.org/10.1016/j.mimet.2012.01.017" @default.
• W2155189368 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22326479" @default.
• W2155189368 hasPublicationYear "2012" @default.
• W2155189368 type Work @default.
• W2155189368 sameAs 2155189368 @default.
• W2155189368 citedByCount "45" @default.
• W2155189368 countsByYear W21551893682013 @default.
• W2155189368 countsByYear W21551893682014 @default.
• W2155189368 countsByYear W21551893682015 @default.
• W2155189368 countsByYear W21551893682017 @default.
• W2155189368 countsByYear W21551893682018 @default.
• W2155189368 countsByYear W21551893682019 @default.
• W2155189368 countsByYear W21551893682020 @default.
• W2155189368 countsByYear W21551893682021 @default.
• W2155189368 countsByYear W21551893682022 @default.
• W2155189368 countsByYear W21551893682023 @default.
• W2155189368 crossrefType "journal-article" @default.
• W2155189368 hasAuthorship W2155189368A5005346346 @default.
• W2155189368 hasAuthorship W2155189368A5018853050 @default.
• W2155189368 hasAuthorship W2155189368A5034144223 @default.
• W2155189368 hasAuthorship W2155189368A5048896394 @default.
• W2155189368 hasAuthorship W2155189368A5065656301 @default.
• W2155189368 hasAuthorship W2155189368A5072522026 @default.
• W2155189368 hasAuthorship W2155189368A5085601234 @default.
• W2155189368 hasConcept C104317684 @default.
• W2155189368 hasConcept C125390029 @default.
• W2155189368 hasConcept C193252679 @default.
• W2155189368 hasConcept C2778867309 @default.
• W2155189368 hasConcept C2779430813 @default.
• W2155189368 hasConcept C54355233 @default.
• W2155189368 hasConcept C59822182 @default.
• W2155189368 hasConcept C73573662 @default.
• W2155189368 hasConcept C7452308 @default.
• W2155189368 hasConcept C84699730 @default.
• W2155189368 hasConcept C86803240 @default.
• W2155189368 hasConcept C89423630 @default.
• W2155189368 hasConceptScore W2155189368C104317684 @default.
• W2155189368 hasConceptScore W2155189368C125390029 @default.
• W2155189368 hasConceptScore W2155189368C193252679 @default.
• W2155189368 hasConceptScore W2155189368C2778867309 @default.
• W2155189368 hasConceptScore W2155189368C2779430813 @default.
• W2155189368 hasConceptScore W2155189368C54355233 @default.
• W2155189368 hasConceptScore W2155189368C59822182 @default.
• W2155189368 hasConceptScore W2155189368C73573662 @default.
• W2155189368 hasConceptScore W2155189368C7452308 @default.
• W2155189368 hasConceptScore W2155189368C84699730 @default.
• W2155189368 hasConceptScore W2155189368C86803240 @default.
• W2155189368 hasConceptScore W2155189368C89423630 @default.

• next