Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155208047> ?p ?o ?g. }
- W2155208047 endingPage "4431" @default.
- W2155208047 startingPage "4423" @default.
- W2155208047 abstract "The CRISPR-Cas9 system is a powerful and revolutionary genome-editing tool for eukaryotic genomes, but its use in bacterial genomes is very limited. Here, we investigated the use of the Streptococcus pyogenes CRISPR-Cas9 system in editing the genome of Clostridium cellulolyticum, a model microorganism for bioenergy research. Wild-type Cas9-induced double-strand breaks were lethal to C. cellulolyticum due to the minimal expression of nonhomologous end joining (NHEJ) components in this strain. To circumvent this lethality, Cas9 nickase was applied to develop a single-nick-triggered homologous recombination strategy, which allows precise one-step editing at intended genomic loci by transforming a single vector. This strategy has a high editing efficiency (>95%) even using short homologous arms (0.2 kb), is able to deliver foreign genes into the genome in a single step without a marker, enables precise editing even at two very similar target sites differing by two bases preceding the seed region, and has a very high target site density (median interval distance of 9 bp and 95.7% gene coverage in C. cellulolyticum). Together, these results establish a simple and robust methodology for genome editing in NHEJ-ineffective prokaryotes." @default.
- W2155208047 created "2016-06-24" @default.
- W2155208047 creator A5005333590 @default.
- W2155208047 creator A5011788533 @default.
- W2155208047 creator A5038412005 @default.
- W2155208047 creator A5040034158 @default.
- W2155208047 creator A5058108916 @default.
- W2155208047 creator A5059585326 @default.
- W2155208047 creator A5061973931 @default.
- W2155208047 creator A5073714647 @default.
- W2155208047 creator A5091210737 @default.
- W2155208047 date "2015-07-01" @default.
- W2155208047 modified "2023-10-12" @default.
- W2155208047 title "Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase" @default.
- W2155208047 cites W1842552430 @default.
- W2155208047 cites W1981185465 @default.
- W2155208047 cites W1982846895 @default.
- W2155208047 cites W1991129150 @default.
- W2155208047 cites W1994361737 @default.
- W2155208047 cites W2000046950 @default.
- W2155208047 cites W2003171404 @default.
- W2155208047 cites W2010659234 @default.
- W2155208047 cites W2018371219 @default.
- W2155208047 cites W2021767455 @default.
- W2155208047 cites W2026850911 @default.
- W2155208047 cites W2043635190 @default.
- W2155208047 cites W2045435533 @default.
- W2155208047 cites W2064815984 @default.
- W2155208047 cites W2077381938 @default.
- W2155208047 cites W2077659966 @default.
- W2155208047 cites W2078699438 @default.
- W2155208047 cites W2085019719 @default.
- W2155208047 cites W2090211843 @default.
- W2155208047 cites W2100067322 @default.
- W2155208047 cites W2100818027 @default.
- W2155208047 cites W2101119036 @default.
- W2155208047 cites W2108244474 @default.
- W2155208047 cites W2110178264 @default.
- W2155208047 cites W2112264024 @default.
- W2155208047 cites W2115295125 @default.
- W2155208047 cites W2115420193 @default.
- W2155208047 cites W2119633529 @default.
- W2155208047 cites W2120263648 @default.
- W2155208047 cites W2123026180 @default.
- W2155208047 cites W2123402293 @default.
- W2155208047 cites W2128112204 @default.
- W2155208047 cites W2128758977 @default.
- W2155208047 cites W2128884353 @default.
- W2155208047 cites W2133702185 @default.
- W2155208047 cites W2133908827 @default.
- W2155208047 cites W2135352791 @default.
- W2155208047 cites W2143999510 @default.
- W2155208047 cites W2144297312 @default.
- W2155208047 cites W2145532900 @default.
- W2155208047 cites W2148590898 @default.
- W2155208047 cites W2157441930 @default.
- W2155208047 cites W2158266834 @default.
- W2155208047 cites W2158849520 @default.
- W2155208047 cites W2160318665 @default.
- W2155208047 cites W2167233750 @default.
- W2155208047 cites W2167299815 @default.
- W2155208047 cites W2167380395 @default.
- W2155208047 cites W2169244146 @default.
- W2155208047 cites W2397457481 @default.
- W2155208047 cites W4206892629 @default.
- W2155208047 cites W4238526656 @default.
- W2155208047 cites W4292230674 @default.
- W2155208047 doi "https://doi.org/10.1128/aem.00873-15" @default.
- W2155208047 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4475897" @default.
- W2155208047 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25911483" @default.
- W2155208047 hasPublicationYear "2015" @default.
- W2155208047 type Work @default.
- W2155208047 sameAs 2155208047 @default.
- W2155208047 citedByCount "188" @default.
- W2155208047 countsByYear W21552080472015 @default.
- W2155208047 countsByYear W21552080472016 @default.
- W2155208047 countsByYear W21552080472017 @default.
- W2155208047 countsByYear W21552080472018 @default.
- W2155208047 countsByYear W21552080472019 @default.
- W2155208047 countsByYear W21552080472020 @default.
- W2155208047 countsByYear W21552080472021 @default.
- W2155208047 countsByYear W21552080472022 @default.
- W2155208047 countsByYear W21552080472023 @default.
- W2155208047 crossrefType "journal-article" @default.
- W2155208047 hasAuthorship W2155208047A5005333590 @default.
- W2155208047 hasAuthorship W2155208047A5011788533 @default.
- W2155208047 hasAuthorship W2155208047A5038412005 @default.
- W2155208047 hasAuthorship W2155208047A5040034158 @default.
- W2155208047 hasAuthorship W2155208047A5058108916 @default.
- W2155208047 hasAuthorship W2155208047A5059585326 @default.
- W2155208047 hasAuthorship W2155208047A5061973931 @default.
- W2155208047 hasAuthorship W2155208047A5073714647 @default.
- W2155208047 hasAuthorship W2155208047A5091210737 @default.
- W2155208047 hasBestOaLocation W21552080471 @default.
- W2155208047 hasConcept C102744134 @default.
- W2155208047 hasConcept C104317684 @default.
- W2155208047 hasConcept C132455925 @default.
- W2155208047 hasConcept C141231307 @default.