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- W2155209250 abstract "Mammary epithelia produce an isotonic, low-Na + fluid that is rich in nutrients. Mechanisms that account for the low electrolyte concentration have not been elucidated, although amiloride-sensitive ion transport has been reported in some situations. We hypothesized that corticosteroid exposure modulates epithelial Na + channel (ENaC) expression and/or activity in bovine mammary epithelial cells. BME-UV cells were grown to confluent monolayers on permeable supports with a standard basolateral medium and apical medium of low-electrolyte, high-lactose composition that resembles the ionic composition of milk. Ion transport was assessed in modified Ussing flux chambers. Exposure to glucocorticoids (dexamethasone, cortisol, or prednisolone), but not aldosterone, increased short-circuit current ( I sc ), a sensitive measure of net ion transport, whereas apical exposure to amiloride or benzamil reduced corticosteroid-induced I sc close to basal levels. Quantitative RT-PCR indicated a glucocorticoid-induced increase in mRNA for β- and γ-ENaC, whereas α-ENaC mRNA expression was only mildly affected. Exposure to mifepristone (a glucocorticoid receptor antagonist), but not spironolactone (a mineralocorticoid receptor antagonist), precluded both the corticosteroid-induced elevation in amiloride-sensitive I sc and the induced changes in β- and γ-ENaC mRNA. We conclude that Na + movement across mammary epithelia is modulated by corticosteroids via a glucocorticoid receptor-mediated mechanism that regulates the expression of the β- and γ-subunits of ENaC. ENaC expression and activity could account for the low Na + concentration that is typical of milk." @default.
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- W2155209250 date "2007-05-01" @default.
- W2155209250 modified "2023-09-25" @default.
- W2155209250 title "Glucocorticoids stimulate ENaC upregulation in bovine mammary epithelium" @default.
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- W2155209250 doi "https://doi.org/10.1152/ajpcell.00369.2006" @default.
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