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• W2155222839 endingPage "1343" @default.
• W2155222839 startingPage "1336" @default.
• W2155222839 abstract "The ability of six antibiotics from different classes to release radiolabeled lipopolysaccharide (LPS) from a phenotypically smooth galE mutant of Escherichia coli O111:B4 was examined. Antibiotic concentrations were 0.0625-512 micrograms/mL. LPS release increased as a function of the antibiotic concentration, reaching a limit at or near the concentration that killed the majority of bacteria. The maximum amount of LPS released by polymyxin B was 40.6% +/- 0.9%, by gentamicin 58.2% +/- 2.5%, by ciprofloxacin 65.8% +/- 2.5%, by ceftazidime 73.1% +/- 0.9%, by tetracycline 75.3% +/- 10.0%, and by imipenem 79.7% +/- 2.3%. In timed experiments, ceftazidime released 61.9% +/- 1.2%, imipenem 51.1% +/- 8.8%, and tetracycline 39.7% +/- 4.4% of the LPS within the first hour of incubation, whereas polymyxin B released 13.5% +/- 1.9%, gentamicin 9.8% +/- 3.6%, and ciprofloxacin 12.7% +/- 2.6% of the LPS (P < .05). Fluoro-radiography and immunoblot analyses revealed similar migration patterns for antibiotic-released and cell-bound LPS on SDS-PAGE gels, suggesting similar O-polysaccharide content in the two LPS fractions. The amount and rate of LPS release from an E. coli strain was dependent upon antibiotic class and concentration." @default.
• W2155222839 created "2016-06-24" @default.
• W2155222839 creator A5052776183 @default.
• W2155222839 creator A5074422550 @default.
• W2155222839 date "1993-06-01" @default.
• W2155222839 modified "2023-09-25" @default.
• W2155222839 title "Effect of Antibiotic Class and Concentration on the Release of Lipopolysaccharide from Escherichia coli" @default.
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• W2155222839 doi "https://doi.org/10.1093/infdis/167.6.1336" @default.
• W2155222839 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8501323" @default.
• W2155222839 hasPublicationYear "1993" @default.
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