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• W2155232493 abstract "A phosphatase secreted by Staphylococcus aureus strain 154 has previously been characterized and classified as a new member of the bacterial class C family of nonspecific acid phosphatases. As the acid phosphatase activity can be easily detected with a cost-effective plate screen assay, quantitatively measured by a simple enzyme assay, and detected by zymography, its potential use as a reporter system was investigated. The S. aureus acid phosphatase (sapS) gene has been cloned and expressed from its own regulatory sequences in Escherichia coli, Bacillus subtilis, and Bacillus halodurans. Transcriptional and translational fusions of the sapS gene with selected heterologous promoters and signal sequences were constructed and expressed in all three of the host strains. From the range of promoters evaluated, the strongest promoter for heterologous protein production in each of the host strains was identified, i.e., the E. coli lacZ promoter in E. coli, the B. halodurans alkaline protease promoter in B. subtilis, and the B. halodurans sigma(D) promoter in B. halodurans. This is the first report on the development of a class C acid phosphatase gene as a reporter gene with the advantage of being able to function in both gram-positive and gram-negative host strains." @default.
• W2155232493 created "2016-06-24" @default.
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• W2155232493 date "2007-11-15" @default.
• W2155232493 modified "2023-09-26" @default.
• W2155232493 title "Evaluation of the <i>Staphylococcus aureus</i> Class C Nonspecific Acid Phosphatase (SapS) as a Reporter for Gene Expression and Protein Secretion in Gram-Negative and Gram-Positive Bacteria" @default.
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• W2155232493 doi "https://doi.org/10.1128/aem.01030-07" @default.
• W2155232493 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2168221" @default.
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• W2155232493 hasPublicationYear "2007" @default.
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