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• W2155240914 abstract "Sera from Canadian pigs (brucellosis free, n = 14 037) and sera from pigs infected with Brucella suis (n = 401) were tested by the buffered antigen plate agglutination test, the complement fixation test, an indirect and a competitive enzyme immunoassay and a fluorescence polarization assay. The results were analysed and assay sensitivity and specificity estimates were calculated. The sensitivity and specificity of the tests were as follows: the buffered antigen plate agglutination test, 77.1 and 96.9%; the complement fixation test (considering anticomplementary sera as negative), 93.3 and 95.5%; the complement fixation test (considering anticomplementary sera as positive), 58.1 and 99.9%; the indirect enzyme immunoassay, 94.0 and 97.9%; the competitive enzyme immunoassay, 90.8 and 96.6%; and the fluorescence polarization assay, 93.5 and 97.2%; respectively. It was concluded that the fluorescence polarization assay was a valuable asset to the diagnosis of porcine brucellosis because of its accuracy, ease of performance and relative cost." @default.
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• W2155240914 date "1999-08-01" @default.
• W2155240914 modified "2023-10-01" @default.
• W2155240914 title "Validation of the fluorescence polarization assay as a serological test for the presumptive diagnosis of porcine brucellosis" @default.
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• W2155240914 doi "https://doi.org/10.1016/s0378-1135(99)00077-2" @default.
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