Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155309949> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2155309949 endingPage "961" @default.
- W2155309949 startingPage "955" @default.
- W2155309949 abstract "We have demonstrated that an active enzyme can be expressed on the surface of a bacteriophage. The gene encoding alkaline phosphatase from Escherichia coli was cloned upstream of gene 3, which encodes a minor coat protein of the filamentous bacteriophage, fd. A fusion protein of the correct size was detected from viral particles by Western blotting. Ultrafiltration confirmed that the enzyme fusion behaves as part of a larger structure as would be expected of an enzyme fused to a viral particle. Both wild-type alkaline phosphatase (Arg166) and an active site mutant (Ala166) expressed in this way retain catalytic activity and have qualitatively similar kinetic properties to free enzyme. Values were obtained for Km of 72.7 and 1070 microM respectively whilst relative kcat for the mutant was 36% of that for wild-type. Phage particles expressing alkaline phosphatase were bound to an immobilized inhibitor (arsenate-Sepharose) and eluted with product (20 mM inorganic phosphate). In this way, the functional enzyme is co-purified with the DNA encoding it. This may permit a novel approach to enzyme engineering based on affinity chromatography of mutant enzymes expressed on the phage surface." @default.
- W2155309949 created "2016-06-24" @default.
- W2155309949 creator A5063135395 @default.
- W2155309949 creator A5073459328 @default.
- W2155309949 creator A5088278689 @default.
- W2155309949 date "1991-01-01" @default.
- W2155309949 modified "2023-10-14" @default.
- W2155309949 title "Phage-enzymes: expression and affinity chromatography of functional alkaline phosphatase on the surface of bacteriophage" @default.
- W2155309949 doi "https://doi.org/10.1093/protein/4.8.955" @default.
- W2155309949 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1817259" @default.
- W2155309949 hasPublicationYear "1991" @default.
- W2155309949 type Work @default.
- W2155309949 sameAs 2155309949 @default.
- W2155309949 citedByCount "118" @default.
- W2155309949 countsByYear W21553099492013 @default.
- W2155309949 countsByYear W21553099492014 @default.
- W2155309949 countsByYear W21553099492015 @default.
- W2155309949 countsByYear W21553099492019 @default.
- W2155309949 countsByYear W21553099492021 @default.
- W2155309949 countsByYear W21553099492022 @default.
- W2155309949 crossrefType "journal-article" @default.
- W2155309949 hasAuthorship W2155309949A5063135395 @default.
- W2155309949 hasAuthorship W2155309949A5073459328 @default.
- W2155309949 hasAuthorship W2155309949A5088278689 @default.
- W2155309949 hasConcept C104317684 @default.
- W2155309949 hasConcept C143065580 @default.
- W2155309949 hasConcept C153911025 @default.
- W2155309949 hasConcept C160160445 @default.
- W2155309949 hasConcept C178666793 @default.
- W2155309949 hasConcept C179247698 @default.
- W2155309949 hasConcept C181199279 @default.
- W2155309949 hasConcept C185592680 @default.
- W2155309949 hasConcept C2776441376 @default.
- W2155309949 hasConcept C547475151 @default.
- W2155309949 hasConcept C55493867 @default.
- W2155309949 hasConcept C86803240 @default.
- W2155309949 hasConceptScore W2155309949C104317684 @default.
- W2155309949 hasConceptScore W2155309949C143065580 @default.
- W2155309949 hasConceptScore W2155309949C153911025 @default.
- W2155309949 hasConceptScore W2155309949C160160445 @default.
- W2155309949 hasConceptScore W2155309949C178666793 @default.
- W2155309949 hasConceptScore W2155309949C179247698 @default.
- W2155309949 hasConceptScore W2155309949C181199279 @default.
- W2155309949 hasConceptScore W2155309949C185592680 @default.
- W2155309949 hasConceptScore W2155309949C2776441376 @default.
- W2155309949 hasConceptScore W2155309949C547475151 @default.
- W2155309949 hasConceptScore W2155309949C55493867 @default.
- W2155309949 hasConceptScore W2155309949C86803240 @default.
- W2155309949 hasIssue "8" @default.
- W2155309949 hasLocation W21553099491 @default.
- W2155309949 hasLocation W21553099492 @default.
- W2155309949 hasOpenAccess W2155309949 @default.
- W2155309949 hasPrimaryLocation W21553099491 @default.
- W2155309949 hasRelatedWork W1570205898 @default.
- W2155309949 hasRelatedWork W1584729042 @default.
- W2155309949 hasRelatedWork W2114402394 @default.
- W2155309949 hasRelatedWork W2360987025 @default.
- W2155309949 hasRelatedWork W2382269147 @default.
- W2155309949 hasRelatedWork W2387178301 @default.
- W2155309949 hasRelatedWork W2785993273 @default.
- W2155309949 hasRelatedWork W2992955585 @default.
- W2155309949 hasRelatedWork W3144733943 @default.
- W2155309949 hasRelatedWork W4283722671 @default.
- W2155309949 hasVolume "4" @default.
- W2155309949 isParatext "false" @default.
- W2155309949 isRetracted "false" @default.
- W2155309949 magId "2155309949" @default.
- W2155309949 workType "article" @default.