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- W2155322419 abstract "ABSTRACT Two different humanized immunoglobulin G1(κ) antibodies and an Fab′ fragment were produced by Aspergillus niger . The antibodies were secreted into the culture supernatant. Both light and heavy chains were initially synthesized as fusion proteins with native glucoamylase. After antibody assembly, cleavage by A. niger KexB protease allowed the release of free antibody. Purification by hydrophobic charge induction chromatography proved effective at removing any antibody to which glucoamylase remained attached. Glycosylation at N297 in the Fc region of the heavy chain was observed, but this site was unoccupied on approximately 50% of the heavy chains. The glycan was of the high-mannose type, with some galactose present, and the size ranged from Hex 6 GlcNAc 2 to Hex 15 GlcNAc 2 . An aglycosyl mutant form of antibody was also produced. No significant difference between the glycosylated antibody produced by Aspergillus and that produced by mammalian cell cultures was observed in tests for affinity, avidity, pharmacokinetics, or antibody-dependent cellular cytotoxicity function." @default.
- W2155322419 created "2016-06-24" @default.
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- W2155322419 date "2004-05-01" @default.
- W2155322419 modified "2023-10-01" @default.
- W2155322419 title "Characterization of Humanized Antibodies Secreted by<i>Aspergillus niger</i>" @default.
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- W2155322419 doi "https://doi.org/10.1128/aem.70.5.2567-2576.2004" @default.
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