FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155335956> ?p ?o ?g. }

• W2155335956 endingPage "1786" @default.
• W2155335956 startingPage "1775" @default.
• W2155335956 abstract "Oocyte maturation and somatic cell nuclear transfer (NT) studies conducted in the domestic cat can provide valuable insights that are relevant to the conservation of endangered species of felids. The present investigation focuses on the in vitro maturation (IVM) of domestic cat oocytes stimulated by insulin-like growth factor-I (IGF-I) and their possible use as recipient cytoplasts for somatic cell NT. In Experiment I, the effects of IGF-I on cat oocyte IVM were monitored. Cumulus-oocyte complexes (COCs) were recovered in TALP-HEPES medium following ovarian follicular aspiration and were classified under a stereomicroscope into four grades using criteria based on cumulus cell investment and the uniformity of ooplasm. The COCs were either cultured in Dulbecco's modified Eagle medium (DMEM) alone as a control group or supplemented with 100 ng/ml IGF-I. After culturing for 32-34 h, oocytes were denuded and maturation rate was evaluated by observing the extrusion of the first polar body and staining with aceto-orcein. The percentages of maturation of Grades 1 and 2 oocytes were significantly increased (P<0.05) in IGF-I supplemented medium compared with medium alone (85.8 versus 65.5 and 70.3 versus 51.8, respectively) whereas the maturation rates of Grades 3 and 4 oocytes were not different. The IVM of Grade 1 oocytes was significantly higher (P<0.05) than for all other grades in both control and experimental groups. In Experiment II, the in vitro development of cat NT embryos using cumulus cells, fetal or adult fibroblasts as donor nuclei was investigated. The IVM oocytes in medium containing IGF-I were enucleated and fused with cumulus cells, fetal or adult fibroblasts between passages 2 and 4 of culture. Reconstructed embryos were cultured and monitored every 24h for progression of development through Day 9. There was no significant difference in the percentage of fusion of NT embryos using different donor nuclei whereas the cleavage rates of NT embryos reconstructed with fetal fibroblasts and cumulus cells were significantly higher (P<0.05) than those reconstructed with adult fibroblasts (72.5 and 70.7% versus 54.8%, respectively). Development of NT embryos reconstructed with adult fibroblast to the morula stage was significantly lower (P<0.05) compared with cumulus cell or fetal fibroblast donor cells (25.8% versus 37.9 or 47.5%, respectively). However, no difference was observed in development to the blastocyst stage. These results demonstrated that IGF-I promoted the IVM of domestic cat oocytes. The enucleated IVM oocytes could be used as recipient cytoplasm for fetal and adult somatic cell nuclei resulting in the production of cloned cat embryos." @default.
• W2155335956 created "2016-06-24" @default.
• W2155335956 creator A5002634330 @default.
• W2155335956 creator A5008811491 @default.
• W2155335956 creator A5013963831 @default.
• W2155335956 date "2003-04-01" @default.
• W2155335956 modified "2023-10-17" @default.
• W2155335956 title "Somatic cell nuclear transfer in domestic cat oocytes treated with IGF-I for in vitro maturation" @default.
• W2155335956 cites W1487014009 @default.
• W2155335956 cites W1585827177 @default.
• W2155335956 cites W1968046875 @default.
• W2155335956 cites W1970894097 @default.
• W2155335956 cites W1972962741 @default.
• W2155335956 cites W1977723116 @default.
• W2155335956 cites W1989449490 @default.
• W2155335956 cites W1990492885 @default.
• W2155335956 cites W1992525863 @default.
• W2155335956 cites W1993205220 @default.
• W2155335956 cites W2003701368 @default.
• W2155335956 cites W2006492607 @default.
• W2155335956 cites W2022488394 @default.
• W2155335956 cites W2030696630 @default.
• W2155335956 cites W2030771022 @default.
• W2155335956 cites W2031776763 @default.
• W2155335956 cites W2033404814 @default.
• W2155335956 cites W2035410480 @default.
• W2155335956 cites W2037457080 @default.
• W2155335956 cites W2038718973 @default.
• W2155335956 cites W2040309689 @default.
• W2155335956 cites W2040855835 @default.
• W2155335956 cites W2044769116 @default.
• W2155335956 cites W2046483541 @default.
• W2155335956 cites W2051659348 @default.
• W2155335956 cites W2062462448 @default.
• W2155335956 cites W2063404951 @default.
• W2155335956 cites W2066707862 @default.
• W2155335956 cites W2068196702 @default.
• W2155335956 cites W2068779000 @default.
• W2155335956 cites W2073294799 @default.
• W2155335956 cites W2088248895 @default.
• W2155335956 cites W2090578620 @default.
• W2155335956 cites W2092310898 @default.
• W2155335956 cites W2107645087 @default.
• W2155335956 cites W2108372662 @default.
• W2155335956 cites W2120490784 @default.
• W2155335956 cites W2122529144 @default.
• W2155335956 cites W2128997939 @default.
• W2155335956 cites W2131347588 @default.
• W2155335956 cites W2132706361 @default.
• W2155335956 cites W2133073488 @default.
• W2155335956 cites W2135609140 @default.
• W2155335956 cites W2136379538 @default.
• W2155335956 cites W2138580885 @default.
• W2155335956 cites W2141926462 @default.
• W2155335956 cites W2147940687 @default.
• W2155335956 cites W2160162034 @default.
• W2155335956 cites W2168398734 @default.
• W2155335956 cites W2170313975 @default.
• W2155335956 cites W2332230337 @default.
• W2155335956 cites W2419090350 @default.
• W2155335956 cites W2528048056 @default.
• W2155335956 cites W4248948939 @default.
• W2155335956 cites W2012994599 @default.
• W2155335956 doi "https://doi.org/10.1016/s0093-691x(02)01235-9" @default.
• W2155335956 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12566151" @default.
• W2155335956 hasPublicationYear "2003" @default.
• W2155335956 type Work @default.
• W2155335956 sameAs 2155335956 @default.
• W2155335956 citedByCount "60" @default.
• W2155335956 countsByYear W21553359562012 @default.
• W2155335956 countsByYear W21553359562014 @default.
• W2155335956 countsByYear W21553359562016 @default.
• W2155335956 countsByYear W21553359562018 @default.
• W2155335956 countsByYear W21553359562019 @default.
• W2155335956 countsByYear W21553359562020 @default.
• W2155335956 countsByYear W21553359562021 @default.
• W2155335956 countsByYear W21553359562022 @default.
• W2155335956 crossrefType "journal-article" @default.
• W2155335956 hasAuthorship W2155335956A5002634330 @default.
• W2155335956 hasAuthorship W2155335956A5008811491 @default.
• W2155335956 hasAuthorship W2155335956A5013963831 @default.
• W2155335956 hasConcept C104317684 @default.
• W2155335956 hasConcept C134305767 @default.
• W2155335956 hasConcept C16685009 @default.
• W2155335956 hasConcept C196843134 @default.
• W2155335956 hasConcept C2775966511 @default.
• W2155335956 hasConcept C2776690073 @default.
• W2155335956 hasConcept C55493867 @default.
• W2155335956 hasConcept C71924100 @default.
• W2155335956 hasConcept C86803240 @default.
• W2155335956 hasConcept C95444343 @default.
• W2155335956 hasConceptScore W2155335956C104317684 @default.
• W2155335956 hasConceptScore W2155335956C134305767 @default.
• W2155335956 hasConceptScore W2155335956C16685009 @default.
• W2155335956 hasConceptScore W2155335956C196843134 @default.
• W2155335956 hasConceptScore W2155335956C2775966511 @default.
• W2155335956 hasConceptScore W2155335956C2776690073 @default.
• W2155335956 hasConceptScore W2155335956C55493867 @default.

• next