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• W2155344313 abstract "Abstract: The accumulation of inositol phosphates (IPs) in response to prostaglandins (PGs) was studied in NG108-15 cells preincubated with myo-[3H]inositol. As a positive control, bradykinin caused accumulation of IPs transiently at an early phase (within 1 min) and continuously during a late phase (15-60 min) of incubation in the cells. PGD2 and PGF2adid not significantly cause the accumulation of IPs at an early phase but significantly stimulated inositol bisphosphate (IP2) and inositol monophosphate (IP1) formation at a late phase of incubation. The maximum stimulation was obtained at <10−7M concentrations of these PGs. the levels being three-and twofold for IP2 and IP1 respectively. 9α, 11 β-PGF2 has a slight effect but PGE2 and the metabolites of PGD2 and PGF2α have no effect up to 10−6M. The effects of PGD2 and PGF2α were not additive, but the effect of each PG was additive to that of bradykinin at a late phase of incubation. Inositol 1-monophosphate was mainly identified in the stimulation by 10−5 M PGD2 and 10−5MPGF2a, whereas both inositol 1-monophosphate and inositol 4-monophosphate were produced in the stimulation by 10−5M bradykinin. Depletion of extracellular Ca2+ diminished the stimulatory effect of PGD2 and PGF2α and late-phase effect of bradykinin, but simple Ca2+ influx into the cells by high K+, ionomycin, or A23187 failed to cause such late-phase effects. These results suggest that PGD2 and PGF2α specifically stimulate hydrolysis of inositol phospholipids." @default.
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• W2155344313 date "1989-11-01" @default.
• W2155344313 modified "2023-10-01" @default.
• W2155344313 title "Late-Phase Accumulation of Inositol Phosphates Stimulated by Prostaglandins D2and F2αin Neuroblastoma × Glioma Hybrid NG108-15 Cells" @default.
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• W2155344313 doi "https://doi.org/10.1111/j.1471-4159.1989.tb08537.x" @default.
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