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• W2155364072 abstract "SPECIFIC AIMSThe central aim of this study was to determine whether activation of Src regulates agonist- and growth factor-induced human airway smooth muscle (ASM) cell proliferation and migration and whether agonist-specific activation of Src underlies differential proliferative and migratory responses.PRINCIPAL FINDINGS1. Src is necessary and sufficient for proliferation of human ASM cellsSince Src regulation of growth factor-induced DNA synthesis is cell type specific, we investigated whether Src mediates ASM cell growth induced by PDGF, EGF, or thrombin using pharmacological and molecular approaches. In serum-deprived ASM cells, pretreatment with PP2, a Src inhibitor, followed by stimulation of cell monolayers with PDGF, EGF, or thrombin, PP2 abrogated PDGF-, thrombin-, and EGF-induced DNA synthesis measured by [3H]-thymidine incorporation in a concentration-dependent manner. In parallel experiments, PP2 inhibited mitogen-induced cell proliferation as demonstrated by cell counting. The IC50 for PDGF-,..." @default.
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• W2155364072 date "2004-12-20" @default.
• W2155364072 modified "2023-10-15" @default.
• W2155364072 title "Src is necessary and sufficient for human airway smooth muscle cell proliferation and migration" @default.
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• W2155364072 doi "https://doi.org/10.1096/fj.04-2869fje" @default.
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