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- W2155368930 abstract "The kinetics and nature of glycolate uptake by the marine diatom Thallassiosira pseudoncma were examined both in the light and dark. After a 2 h incubation with [1-14C]lglycolate in the light, the major part of the radioactive label taken up was located in the protein fraction of the cells. The metabolic inhibitors isonicotinic acid hydrazide (INH) and a-hydroxypyridyl methanesulphonate (α-HPMS) reduced the total assimilation of 14C from [1-14C]glycolate and reduced the percentage of radioactivity found in the protein fraction. Short-term kinetic experiments showed that [1-14C]lglycolate was accumulated in the cells unchanged and after 30 s the glycolate pathway intermediates glycine and serine became labelled. After 4 min serine was the dominant compound labelled, with small quantities of labelled glycine also present. The enzyme glycolate: DCPIP oxidoreductase was shown to be present in high enough concentrations to account for the observed glycolate assimilation. These results substantiate the existence of the glycolic acid pathway in Th. pseudonana." @default.
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- W2155368930 date "1977-01-01" @default.
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- W2155368930 title "Glycolate Metabolism inThallassiosira pseudonana" @default.
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- W2155368930 doi "https://doi.org/10.1093/jxb/28.1.78" @default.
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