FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155384802> ?p ?o ?g. }

• W2155384802 endingPage "442" @default.
• W2155384802 startingPage "431" @default.
• W2155384802 abstract "Sympathetic neurons internalize nerve growth factor (NGF) and transport it retrogradely to their cell bodies where it appears to serve a trophic function in maintaining neuronal survival. We have characterized the binding, internalization, and retrograde transport of 125I-NGF by cultured rat sympathetic neurons. After 3 to 4 weeks in culture, sympathetic neurons possessed approximately 2 X 10(7) specific, cell surface NGF binding sites per neuron with an apparent affinity constant of 2 to 5 X 10(9) M. The density of binding sites on the plasma membrane of the neurites approximately twice that on the plasma membrane of the cell bodies. Because of the extensive network of neuronal processes, the neurites probably account for more than 99.5% of the total binding in mature cultures. Using electron microscope autoradiography, we localized the distribution of 125I-NGF in the cell body following a 1-hr exposure to 125I-NGF. The majority of silver grains were associated with lysosomal organelles, including secondary lysosomes, residual bodies, and multivesicular bodies (MVB). The MVB were the most heavily labeled, with a labeling density (L.D.) of 21, while the lysosomes had a L.D. of 3.1. To study the retrograde transport of 125I-NGF, neurons were grown in compartmentalized culture dishes and their distal processes were exposed to 125I-NGF. Radioactive material was transported to the cell bodies at the rate of approximately 3 mm/hr. The transport mechanism was sensitive to colchicine and was saturable with respect to NGF. After 8 hr of transport, when the radioactivity in the cell bodies had reached a steady state, the label again was localized primarily to the MVB (L.D. = 16.8) and the lysosomes (L.D. = 3.8). The nuclei were not labeled significantly and had an overall L.D. of 0.47. We saw no evidence for the accumulation of NGF by the nuclear membrane, the nucleolus, or chromatin." @default.
• W2155384802 created "2016-06-24" @default.
• W2155384802 creator A5028982584 @default.
• W2155384802 creator A5031934584 @default.
• W2155384802 creator A5057746394 @default.
• W2155384802 creator A5066084020 @default.
• W2155384802 date "1982-04-01" @default.
• W2155384802 modified "2023-10-13" @default.
• W2155384802 title "Binding, internalization, and retrograde transport of 125I-nerve growth factor in cultured rat sympathetic neurons" @default.
• W2155384802 cites W1486351035 @default.
• W2155384802 cites W1516408301 @default.
• W2155384802 cites W182278069 @default.
• W2155384802 cites W1894641937 @default.
• W2155384802 cites W1965707331 @default.
• W2155384802 cites W1967274915 @default.
• W2155384802 cites W1968860081 @default.
• W2155384802 cites W1973641301 @default.
• W2155384802 cites W1978668606 @default.
• W2155384802 cites W1979979596 @default.
• W2155384802 cites W1982202183 @default.
• W2155384802 cites W1982477399 @default.
• W2155384802 cites W1992623778 @default.
• W2155384802 cites W1994494600 @default.
• W2155384802 cites W1996940538 @default.
• W2155384802 cites W1997458926 @default.
• W2155384802 cites W2006806905 @default.
• W2155384802 cites W2012717250 @default.
• W2155384802 cites W2021080555 @default.
• W2155384802 cites W2025253130 @default.
• W2155384802 cites W2027243420 @default.
• W2155384802 cites W2029006377 @default.
• W2155384802 cites W2029287429 @default.
• W2155384802 cites W2031050497 @default.
• W2155384802 cites W2040796522 @default.
• W2155384802 cites W2043392377 @default.
• W2155384802 cites W2050905439 @default.
• W2155384802 cites W2056821867 @default.
• W2155384802 cites W2058564383 @default.
• W2155384802 cites W2058811899 @default.
• W2155384802 cites W2064973888 @default.
• W2155384802 cites W2070813646 @default.
• W2155384802 cites W2075032348 @default.
• W2155384802 cites W2075783688 @default.
• W2155384802 cites W2078960031 @default.
• W2155384802 cites W2086271423 @default.
• W2155384802 cites W2088969302 @default.
• W2155384802 cites W2093479997 @default.
• W2155384802 cites W2093771709 @default.
• W2155384802 cites W2095020876 @default.
• W2155384802 cites W2132225567 @default.
• W2155384802 cites W2158028807 @default.
• W2155384802 cites W2161106503 @default.
• W2155384802 cites W2174825349 @default.
• W2155384802 cites W2413529773 @default.
• W2155384802 cites W2036707031 @default.
• W2155384802 cites W2180553270 @default.
• W2155384802 doi "https://doi.org/10.1523/jneurosci.02-04-00431.1982" @default.
• W2155384802 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6564245" @default.
• W2155384802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7069465" @default.
• W2155384802 hasPublicationYear "1982" @default.
• W2155384802 type Work @default.
• W2155384802 sameAs 2155384802 @default.
• W2155384802 citedByCount "135" @default.
• W2155384802 countsByYear W21553848022012 @default.
• W2155384802 countsByYear W21553848022013 @default.
• W2155384802 countsByYear W21553848022014 @default.
• W2155384802 countsByYear W21553848022015 @default.
• W2155384802 countsByYear W21553848022016 @default.
• W2155384802 countsByYear W21553848022017 @default.
• W2155384802 countsByYear W21553848022018 @default.
• W2155384802 countsByYear W21553848022021 @default.
• W2155384802 countsByYear W21553848022023 @default.
• W2155384802 crossrefType "journal-article" @default.
• W2155384802 hasAuthorship W2155384802A5028982584 @default.
• W2155384802 hasAuthorship W2155384802A5031934584 @default.
• W2155384802 hasAuthorship W2155384802A5057746394 @default.
• W2155384802 hasAuthorship W2155384802A5066084020 @default.
• W2155384802 hasBestOaLocation W21553848021 @default.
• W2155384802 hasConcept C113246987 @default.
• W2155384802 hasConcept C12554922 @default.
• W2155384802 hasConcept C126322002 @default.
• W2155384802 hasConcept C134018914 @default.
• W2155384802 hasConcept C139770010 @default.
• W2155384802 hasConcept C1491633281 @default.
• W2155384802 hasConcept C168240541 @default.
• W2155384802 hasConcept C169760540 @default.
• W2155384802 hasConcept C170493617 @default.
• W2155384802 hasConcept C185592680 @default.
• W2155384802 hasConcept C202751555 @default.
• W2155384802 hasConcept C2778423431 @default.
• W2155384802 hasConcept C2778794669 @default.
• W2155384802 hasConcept C55493867 @default.
• W2155384802 hasConcept C70192387 @default.
• W2155384802 hasConcept C71924100 @default.
• W2155384802 hasConcept C86803240 @default.
• W2155384802 hasConcept C95444343 @default.
• W2155384802 hasConceptScore W2155384802C113246987 @default.
• W2155384802 hasConceptScore W2155384802C12554922 @default.

• next