Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155385246> ?p ?o ?g. }
- W2155385246 endingPage "489" @default.
- W2155385246 startingPage "479" @default.
- W2155385246 abstract "To develop a quantitative PCR assay for sensitive and specific detection of Mycobacterium avium ssp. paratuberculosis (Map) in a range of dairy products.TaqMan(®) assays were designed to target the IS900 and f57 genetic elements of Map. Both real-time PCR assays were integrated with the Adiapure(®) Map DNA extraction kit and assessed separately for the detection/quantification of Map in spiked milk, Cheddar cheese and milk powder. Assays were validated against Cheddar cheese samples containing known concentrations of Map. The IS900 qPCR assay was significantly more sensitive than the assay based on the f57 primer/probe. At a threshold cycle value of 38, limits of detection (LOD) for the IS900 qPCR assay were 0·6 CFU ml(-1), 2·8 CFU g(-1) and 30 CFU g(-1) for artificially contaminated pasteurized milk, whole milk powder and Cheddar cheese, respectively. The respective LOD's for the f57 assay were 6·2 CFU ml(-1), 26·7 CFU g(-1) and 316 CFU g(-1).The integrated Adiapure(®) extraction - IS900 real time assay described is a sensitive, quantitative method for the detection of Map in dairy products. This is the first study to consider qPCR as a quantitative estimation of Map-DNA in cheese and whole milk powder.The assay developed allows sensitive detection and quantification of Map DNA in a range of dairy products which is valuable for the screening and surveillance of this potential zoonotic organism." @default.
- W2155385246 created "2016-06-24" @default.
- W2155385246 creator A5018564881 @default.
- W2155385246 creator A5049785502 @default.
- W2155385246 creator A5089790970 @default.
- W2155385246 date "2010-12-13" @default.
- W2155385246 modified "2023-10-18" @default.
- W2155385246 title "Detection of Mycobacterium avium ssp. paratuberculosis in cheese, milk powder and milk using IS900 and f57-based qPCR assays" @default.
- W2155385246 cites W1497353324 @default.
- W2155385246 cites W1543022557 @default.
- W2155385246 cites W1579071151 @default.
- W2155385246 cites W1757365063 @default.
- W2155385246 cites W1968520525 @default.
- W2155385246 cites W1977171845 @default.
- W2155385246 cites W1977266238 @default.
- W2155385246 cites W1985807230 @default.
- W2155385246 cites W1986015312 @default.
- W2155385246 cites W1986910386 @default.
- W2155385246 cites W1988338328 @default.
- W2155385246 cites W2008997261 @default.
- W2155385246 cites W2016586930 @default.
- W2155385246 cites W2019773852 @default.
- W2155385246 cites W2020943969 @default.
- W2155385246 cites W2021779499 @default.
- W2155385246 cites W2022400760 @default.
- W2155385246 cites W2026584887 @default.
- W2155385246 cites W2043700585 @default.
- W2155385246 cites W2054460848 @default.
- W2155385246 cites W2055732197 @default.
- W2155385246 cites W2063923295 @default.
- W2155385246 cites W2068306388 @default.
- W2155385246 cites W2080072512 @default.
- W2155385246 cites W2081504479 @default.
- W2155385246 cites W2087962484 @default.
- W2155385246 cites W2089100536 @default.
- W2155385246 cites W2097884021 @default.
- W2155385246 cites W2107006125 @default.
- W2155385246 cites W2114140048 @default.
- W2155385246 cites W2117893874 @default.
- W2155385246 cites W2125397084 @default.
- W2155385246 cites W2127919257 @default.
- W2155385246 cites W2131086733 @default.
- W2155385246 cites W2132076059 @default.
- W2155385246 cites W2135922509 @default.
- W2155385246 cites W2138384048 @default.
- W2155385246 cites W2140534296 @default.
- W2155385246 cites W2144113861 @default.
- W2155385246 cites W2145000793 @default.
- W2155385246 cites W2149607296 @default.
- W2155385246 cites W2151275994 @default.
- W2155385246 cites W2152281664 @default.
- W2155385246 cites W2152472226 @default.
- W2155385246 cites W2160483017 @default.
- W2155385246 cites W2164616601 @default.
- W2155385246 cites W2166104982 @default.
- W2155385246 cites W2166668691 @default.
- W2155385246 cites W2167840222 @default.
- W2155385246 cites W2168391206 @default.
- W2155385246 cites W2171671180 @default.
- W2155385246 cites W2181458284 @default.
- W2155385246 cites W4211007841 @default.
- W2155385246 cites W48066939 @default.
- W2155385246 doi "https://doi.org/10.1111/j.1365-2672.2010.04905.x" @default.
- W2155385246 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21155954" @default.
- W2155385246 hasPublicationYear "2010" @default.
- W2155385246 type Work @default.
- W2155385246 sameAs 2155385246 @default.
- W2155385246 citedByCount "39" @default.
- W2155385246 countsByYear W21553852462014 @default.
- W2155385246 countsByYear W21553852462015 @default.
- W2155385246 countsByYear W21553852462016 @default.
- W2155385246 countsByYear W21553852462017 @default.
- W2155385246 countsByYear W21553852462018 @default.
- W2155385246 countsByYear W21553852462019 @default.
- W2155385246 countsByYear W21553852462020 @default.
- W2155385246 countsByYear W21553852462021 @default.
- W2155385246 countsByYear W21553852462022 @default.
- W2155385246 countsByYear W21553852462023 @default.
- W2155385246 crossrefType "journal-article" @default.
- W2155385246 hasAuthorship W2155385246A5018564881 @default.
- W2155385246 hasAuthorship W2155385246A5049785502 @default.
- W2155385246 hasAuthorship W2155385246A5089790970 @default.
- W2155385246 hasBestOaLocation W21553852461 @default.
- W2155385246 hasConcept C104317684 @default.
- W2155385246 hasConcept C109097599 @default.
- W2155385246 hasConcept C119128265 @default.
- W2155385246 hasConcept C143660080 @default.
- W2155385246 hasConcept C185592680 @default.
- W2155385246 hasConcept C2777849792 @default.
- W2155385246 hasConcept C2780374374 @default.
- W2155385246 hasConcept C2992940123 @default.
- W2155385246 hasConcept C3019530640 @default.
- W2155385246 hasConcept C31903555 @default.
- W2155385246 hasConcept C43617362 @default.
- W2155385246 hasConcept C48023723 @default.
- W2155385246 hasConcept C49105822 @default.
- W2155385246 hasConcept C523546767 @default.
- W2155385246 hasConcept C54355233 @default.