FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155388906> ?p ?o ?g. }

• W2155388906 endingPage "68" @default.
• W2155388906 startingPage "59" @default.
• W2155388906 abstract "Intracellularly applied amphiphilic molecules, such as p-chlorophenoxy acetate (CPA) and octanoate, block various pore-open mutants of CLC-0. The voltage-dependent block of a particular pore-open mutant, E166G, was found to be multiphasic. In symmetrical 140 mM Cl(-), the apparent affinity of the blocker in this mutant increased with a negative membrane potential but, paradoxically, decreased when the negative membrane potential was greater than -80 mV, a phenomenon similar to the blocker punch-through shown in many blocker studies of cation channels. To provide further evidence of the punch-through of CPA and octanoate, we studied the dissociation rate of the blocker from the pore by measuring the time constant of relief from the block under various voltage and ionic conditions. Consistent with the voltage dependence of the effect on the steady-state current, the rate of CPA dissociation from the E166G pore reached a minimum at -80 mV in symmetrical 140 mM Cl(-), and the direction of current recovery suggested that the bound CPA in the pore can dissociate into both intracellular and extracellular solutions. Moreover, the CPA dissociation depends upon the Cl(-) reversal potential with a minimal dissociation rate at a voltage 80 mV more negative than the Cl(-) reversal potential. That the shift of the CPA-dissociation rate follows the Cl(-) gradient across the membrane argues that these blockers can indeed punch through the channel pore. Furthermore, a minimal CPA-dissociation rate at a voltage 80 mV more negative than the Cl(-) reversal potential suggests that the outward blocker movement through the CLC-0 pore is more difficult than the inward movement." @default.
• W2155388906 created "2016-06-24" @default.
• W2155388906 creator A5063658779 @default.
• W2155388906 creator A5077277862 @default.
• W2155388906 date "2008-12-15" @default.
• W2155388906 modified "2023-09-26" @default.
• W2155388906 title "Amphiphilic Blockers Punch through a Mutant CLC-0 Pore" @default.
• W2155388906 cites W1517714742 @default.
• W2155388906 cites W1986740737 @default.
• W2155388906 cites W1998401151 @default.
• W2155388906 cites W2005501525 @default.
• W2155388906 cites W2014671743 @default.
• W2155388906 cites W2042152248 @default.
• W2155388906 cites W2053926890 @default.
• W2155388906 cites W2061206985 @default.
• W2155388906 cites W2072176189 @default.
• W2155388906 cites W2100282855 @default.
• W2155388906 cites W2102375930 @default.
• W2155388906 cites W2103142377 @default.
• W2155388906 cites W2107320528 @default.
• W2155388906 cites W2110518868 @default.
• W2155388906 cites W2118516008 @default.
• W2155388906 cites W2135106801 @default.
• W2155388906 cites W2150344778 @default.
• W2155388906 cites W2162206314 @default.
• W2155388906 cites W2164797647 @default.
• W2155388906 cites W2164818894 @default.
• W2155388906 cites W2166283865 @default.
• W2155388906 cites W2169981344 @default.
• W2155388906 cites W2413213959 @default.
• W2155388906 doi "https://doi.org/10.1085/jgp.200810005" @default.
• W2155388906 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2606939" @default.
• W2155388906 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19088382" @default.
• W2155388906 hasPublicationYear "2008" @default.
• W2155388906 type Work @default.
• W2155388906 sameAs 2155388906 @default.
• W2155388906 citedByCount "11" @default.
• W2155388906 countsByYear W21553889062012 @default.
• W2155388906 countsByYear W21553889062015 @default.
• W2155388906 countsByYear W21553889062017 @default.
• W2155388906 countsByYear W21553889062018 @default.
• W2155388906 countsByYear W21553889062019 @default.
• W2155388906 countsByYear W21553889062020 @default.
• W2155388906 countsByYear W21553889062022 @default.
• W2155388906 crossrefType "journal-article" @default.
• W2155388906 hasAuthorship W2155388906A5063658779 @default.
• W2155388906 hasAuthorship W2155388906A5077277862 @default.
• W2155388906 hasBestOaLocation W21553889061 @default.
• W2155388906 hasConcept C102931765 @default.
• W2155388906 hasConcept C12554922 @default.
• W2155388906 hasConcept C147944092 @default.
• W2155388906 hasConcept C153461711 @default.
• W2155388906 hasConcept C15920480 @default.
• W2155388906 hasConcept C170493617 @default.
• W2155388906 hasConcept C178790620 @default.
• W2155388906 hasConcept C181911157 @default.
• W2155388906 hasConcept C185592680 @default.
• W2155388906 hasConcept C2778071365 @default.
• W2155388906 hasConcept C41625074 @default.
• W2155388906 hasConcept C519063684 @default.
• W2155388906 hasConcept C521977710 @default.
• W2155388906 hasConcept C55493867 @default.
• W2155388906 hasConcept C67407626 @default.
• W2155388906 hasConcept C71240020 @default.
• W2155388906 hasConcept C79879829 @default.
• W2155388906 hasConcept C86803240 @default.
• W2155388906 hasConceptScore W2155388906C102931765 @default.
• W2155388906 hasConceptScore W2155388906C12554922 @default.
• W2155388906 hasConceptScore W2155388906C147944092 @default.
• W2155388906 hasConceptScore W2155388906C153461711 @default.
• W2155388906 hasConceptScore W2155388906C15920480 @default.
• W2155388906 hasConceptScore W2155388906C170493617 @default.
• W2155388906 hasConceptScore W2155388906C178790620 @default.
• W2155388906 hasConceptScore W2155388906C181911157 @default.
• W2155388906 hasConceptScore W2155388906C185592680 @default.
• W2155388906 hasConceptScore W2155388906C2778071365 @default.
• W2155388906 hasConceptScore W2155388906C41625074 @default.
• W2155388906 hasConceptScore W2155388906C519063684 @default.
• W2155388906 hasConceptScore W2155388906C521977710 @default.
• W2155388906 hasConceptScore W2155388906C55493867 @default.
• W2155388906 hasConceptScore W2155388906C67407626 @default.
• W2155388906 hasConceptScore W2155388906C71240020 @default.
• W2155388906 hasConceptScore W2155388906C79879829 @default.
• W2155388906 hasConceptScore W2155388906C86803240 @default.
• W2155388906 hasIssue "1" @default.
• W2155388906 hasLocation W21553889061 @default.
• W2155388906 hasLocation W21553889062 @default.
• W2155388906 hasLocation W21553889063 @default.
• W2155388906 hasLocation W21553889064 @default.
• W2155388906 hasLocation W21553889065 @default.
• W2155388906 hasOpenAccess W2155388906 @default.
• W2155388906 hasPrimaryLocation W21553889061 @default.
• W2155388906 hasRelatedWork W2016247671 @default.
• W2155388906 hasRelatedWork W2024867507 @default.
• W2155388906 hasRelatedWork W2028155512 @default.
• W2155388906 hasRelatedWork W2031418289 @default.
• W2155388906 hasRelatedWork W2055973802 @default.
• W2155388906 hasRelatedWork W2093344519 @default.

• next