Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155391341> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2155391341 abstract "Poor survival and differentiation of grafted dopamine neurons limits the application of clinical transplantation in Parkinson's disease. The survival of grafted dopamine neurons is only improved by a factor of 2–3 by adding neuroprotectants during tissue preparation. We used dye exclusion cell viability and electron microscopy to investigate the effects of the caspase inhibitor ac-YVAD-cmk and the lazaroid tirilazad mesylate on ultrastructural changes in dissociated embryonic mesencephalic cells. In addition, we examined whether the neuroprotectants selectively counteracted specific signs of neurodegeneration. Cell viability decreased significantly over time in both control and treated cell suspensions, but the number of viable cells remaining was significantly higher in tirilazad mesylate-treated cell suspensions. In control samples, the proportion of cells with an ultrastructure consistent with healthy cells decreased from 70%, immediately after dissociation, to 30% after 8 h of incubation. Similar changes were also observed in cell suspensions treated with neuroprotectants. Thus, the neuroprotectants examined did not block the development of specific morphological signs of neurodegeneration. However, when also taking into account that dead cells lysed and disappeared from each cell suspension with time, we found that the total number of remaining viable cells with healthy nuclear chromatin or intact membrane integrity was significantly higher in the tirilazad mesylate-treated group. The results indicate that tirilazad mesylate protects only a small subpopulation of embryonic mesencephalic cells from degeneration induced by mechanical trauma during tissue dissection and dissociation." @default.
- W2155391341 created "2016-06-24" @default.
- W2155391341 creator A5019898192 @default.
- W2155391341 creator A5054048756 @default.
- W2155391341 creator A5072622786 @default.
- W2155391341 date "2003-04-01" @default.
- W2155391341 modified "2023-09-23" @default.
- W2155391341 title "Ultrastructural Characterization of Dissociated Embryonic Ventral Mesencephalic Tissue Treated with Neuroprotectants" @default.
- W2155391341 cites W1518446772 @default.
- W2155391341 cites W1811706139 @default.
- W2155391341 cites W1967721280 @default.
- W2155391341 cites W1983693448 @default.
- W2155391341 cites W1985561353 @default.
- W2155391341 cites W1986868939 @default.
- W2155391341 cites W1987619526 @default.
- W2155391341 cites W2021474417 @default.
- W2155391341 cites W2034915619 @default.
- W2155391341 cites W2038124867 @default.
- W2155391341 cites W2041392863 @default.
- W2155391341 cites W2042817821 @default.
- W2155391341 cites W2047968402 @default.
- W2155391341 cites W2074114519 @default.
- W2155391341 cites W2081373475 @default.
- W2155391341 cites W2083898031 @default.
- W2155391341 cites W2084449394 @default.
- W2155391341 cites W2100479578 @default.
- W2155391341 cites W2100867365 @default.
- W2155391341 cites W2122433628 @default.
- W2155391341 cites W2126711468 @default.
- W2155391341 cites W2129263268 @default.
- W2155391341 cites W2136535847 @default.
- W2155391341 cites W2144135557 @default.
- W2155391341 cites W2155873452 @default.
- W2155391341 cites W2162679852 @default.
- W2155391341 cites W2214349547 @default.
- W2155391341 doi "https://doi.org/10.3727/000000003108746795" @default.
- W2155391341 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12797378" @default.
- W2155391341 hasPublicationYear "2003" @default.
- W2155391341 type Work @default.
- W2155391341 sameAs 2155391341 @default.
- W2155391341 citedByCount "7" @default.
- W2155391341 crossrefType "journal-article" @default.
- W2155391341 hasAuthorship W2155391341A5019898192 @default.
- W2155391341 hasAuthorship W2155391341A5054048756 @default.
- W2155391341 hasAuthorship W2155391341A5072622786 @default.
- W2155391341 hasConcept C104317684 @default.
- W2155391341 hasConcept C126322002 @default.
- W2155391341 hasConcept C142724271 @default.
- W2155391341 hasConcept C145103041 @default.
- W2155391341 hasConcept C1491633281 @default.
- W2155391341 hasConcept C185592680 @default.
- W2155391341 hasConcept C2776925932 @default.
- W2155391341 hasConcept C2779134260 @default.
- W2155391341 hasConcept C2911091166 @default.
- W2155391341 hasConcept C53227056 @default.
- W2155391341 hasConcept C55493867 @default.
- W2155391341 hasConcept C71924100 @default.
- W2155391341 hasConcept C86803240 @default.
- W2155391341 hasConcept C95444343 @default.
- W2155391341 hasConceptScore W2155391341C104317684 @default.
- W2155391341 hasConceptScore W2155391341C126322002 @default.
- W2155391341 hasConceptScore W2155391341C142724271 @default.
- W2155391341 hasConceptScore W2155391341C145103041 @default.
- W2155391341 hasConceptScore W2155391341C1491633281 @default.
- W2155391341 hasConceptScore W2155391341C185592680 @default.
- W2155391341 hasConceptScore W2155391341C2776925932 @default.
- W2155391341 hasConceptScore W2155391341C2779134260 @default.
- W2155391341 hasConceptScore W2155391341C2911091166 @default.
- W2155391341 hasConceptScore W2155391341C53227056 @default.
- W2155391341 hasConceptScore W2155391341C55493867 @default.
- W2155391341 hasConceptScore W2155391341C71924100 @default.
- W2155391341 hasConceptScore W2155391341C86803240 @default.
- W2155391341 hasConceptScore W2155391341C95444343 @default.
- W2155391341 hasLocation W21553913411 @default.
- W2155391341 hasLocation W21553913412 @default.
- W2155391341 hasOpenAccess W2155391341 @default.
- W2155391341 hasPrimaryLocation W21553913411 @default.
- W2155391341 hasRelatedWork W1993740514 @default.
- W2155391341 hasRelatedWork W2033451417 @default.
- W2155391341 hasRelatedWork W2038174987 @default.
- W2155391341 hasRelatedWork W2074591015 @default.
- W2155391341 hasRelatedWork W2127025552 @default.
- W2155391341 hasRelatedWork W2186320088 @default.
- W2155391341 hasRelatedWork W2883509033 @default.
- W2155391341 hasRelatedWork W2885088555 @default.
- W2155391341 hasRelatedWork W2999206621 @default.
- W2155391341 hasRelatedWork W17599192 @default.
- W2155391341 isParatext "false" @default.
- W2155391341 isRetracted "false" @default.
- W2155391341 magId "2155391341" @default.
- W2155391341 workType "article" @default.