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- W2155430725 startingPage "1587" @default.
- W2155430725 abstract "This study investigated the subcellular location of mung bean (Vigna radiata) 8S globulin in transient expression systems as well as in tobacco (Nicotiana tabacum) BY-2 cells and different tissues from a transgenic Arabidopsis (Arabidopsis thaliana) line stably expressing this storage globulin. When transiently expressed in protoplasts from both BY-2 cells and Arabidopsis suspension cultured cells, the 8S globulin located to structures that were neither Golgi nor pre-vacuolar compartments (PVCs). Immunogold electron microscopy of the transgenics reveals the 8S globulin-positive structures to be small, spherical, ribosome-covered endoplasmic reticulum (ER)-derived bodies. In BY-2 cells and all vegetative cells, the 8S globulin was present as a pro-form. However, in Arabidopsis embryos, with the onset of endogenous storage protein synthesis, the 8S globulin exited the ER and passed through the PVC to the protein storage vacuole where it was processed to its smaller mature form. These results clearly demonstrated that, when taken out of context and expressed in vegetative cells, the mung bean 8S storage globulin cannot exit the ER, and indicate that natural targeting of storage proteins to the vacuole should be better studied in the maturing seed." @default.
- W2155430725 created "2016-06-24" @default.
- W2155430725 creator A5002465763 @default.
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- W2155430725 creator A5071688219 @default.
- W2155430725 creator A5074458530 @default.
- W2155430725 creator A5076500400 @default.
- W2155430725 date "2013-02-04" @default.
- W2155430725 modified "2023-09-30" @default.
- W2155430725 title "Successful transport to the vacuole of heterologously expressed mung bean 8S globulin occurs in seed but not in vegetative tissues" @default.
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- W2155430725 doi "https://doi.org/10.1093/jxb/ert014" @default.
- W2155430725 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3617825" @default.
- W2155430725 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23382549" @default.
- W2155430725 hasPublicationYear "2013" @default.
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