FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155459051> ?p ?o ?g. }

• W2155459051 endingPage "1408" @default.
• W2155459051 startingPage "1399" @default.
• W2155459051 abstract "The vast majority of DNA polymerases use the complementary templating strand of DNA to guide each nucleotide incorporation. There are instances, however, in which polymerases can efficiently incorporate nucleotides in the absence of templating information. This process, known as translesion DNA synthesis, can alter the proper genetic code of an organism. To further elucidate the mechanism of template-independent DNA synthesis, we monitored the incorporation of various nucleotides at the “blunt-end” of duplex DNA by the high-fidelity bacteriophage T4 DNA polymerase. Although natural nucleotides are not incorporated at the blunt-end, a limited subset of non-natural indolyl analogues containing extensive π-electron surface areas are efficiently utilized by the T4 DNA polymerase. These analogues possess high binding affinities that are remarkably similar to those measured during incorporation opposite an abasic site. In contrast, the kpol values are significantly lower during blunt-end extension when compared to incorporation opposite an abasic site. These kinetic differences suggest that the single-stranded region of the DNA template plays an important role during polymerization through stacking interactions with downstream bases, interactions with key amino acid residues, or both. In addition, we demonstrate that terminal deoxynucleotide transferase, a template-independent enzyme, can efficiently incorporate many of these non-natural nucleotides. However, that this unique polymerase cannot extend large, bulky non-natural nucleotides suggests that elongation is limited by steric constraints imposed by structural features present within the polymerase. Regardless, the kinetic data obtained from using either DNA polymerase indicate that template-independent synthesis can occur without the contributions of hydrogen-bonding interactions and suggest that π-electron interactions play an important role in polymerization efficiency when templating information is not present." @default.
• W2155459051 created "2016-06-24" @default.
• W2155459051 creator A5058712621 @default.
• W2155459051 creator A5077285970 @default.
• W2155459051 date "2007-08-13" @default.
• W2155459051 modified "2023-09-23" @default.
• W2155459051 title "The Use of Non-natural Nucleotides to Probe Template-Independent DNA Synthesis" @default.
• W2155459051 cites W1533555166 @default.
• W2155459051 cites W1670478304 @default.
• W2155459051 cites W1963926318 @default.
• W2155459051 cites W1966195927 @default.
• W2155459051 cites W1967941593 @default.
• W2155459051 cites W1972821008 @default.
• W2155459051 cites W1978649037 @default.
• W2155459051 cites W1992085308 @default.
• W2155459051 cites W1994381045 @default.
• W2155459051 cites W1996371195 @default.
• W2155459051 cites W2004535059 @default.
• W2155459051 cites W2005382394 @default.
• W2155459051 cites W2007959652 @default.
• W2155459051 cites W2009639920 @default.
• W2155459051 cites W2017950533 @default.
• W2155459051 cites W2022953065 @default.
• W2155459051 cites W2029578044 @default.
• W2155459051 cites W2041921960 @default.
• W2155459051 cites W2043070639 @default.
• W2155459051 cites W2046294135 @default.
• W2155459051 cites W2051316365 @default.
• W2155459051 cites W2062881060 @default.
• W2155459051 cites W2068130878 @default.
• W2155459051 cites W2069947233 @default.
• W2155459051 cites W2070226713 @default.
• W2155459051 cites W2071034307 @default.
• W2155459051 cites W2076058601 @default.
• W2155459051 cites W2081653306 @default.
• W2155459051 cites W2093448309 @default.
• W2155459051 cites W2103585578 @default.
• W2155459051 cites W2107119563 @default.
• W2155459051 cites W2111754594 @default.
• W2155459051 cites W2127753323 @default.
• W2155459051 cites W2131566401 @default.
• W2155459051 cites W2153039677 @default.
• W2155459051 cites W2157114285 @default.
• W2155459051 cites W2158207944 @default.
• W2155459051 cites W2165641154 @default.
• W2155459051 cites W2171326992 @default.
• W2155459051 cites W2405845254 @default.
• W2155459051 cites W2411338479 @default.
• W2155459051 cites W4253381965 @default.
• W2155459051 doi "https://doi.org/10.1002/cbic.200700096" @default.
• W2155459051 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17607682" @default.
• W2155459051 hasPublicationYear "2007" @default.
• W2155459051 type Work @default.
• W2155459051 sameAs 2155459051 @default.
• W2155459051 citedByCount "24" @default.
• W2155459051 countsByYear W21554590512012 @default.
• W2155459051 countsByYear W21554590512013 @default.
• W2155459051 countsByYear W21554590512016 @default.
• W2155459051 countsByYear W21554590512017 @default.
• W2155459051 countsByYear W21554590512018 @default.
• W2155459051 countsByYear W21554590512019 @default.
• W2155459051 countsByYear W21554590512022 @default.
• W2155459051 crossrefType "journal-article" @default.
• W2155459051 hasAuthorship W2155459051A5058712621 @default.
• W2155459051 hasAuthorship W2155459051A5077285970 @default.
• W2155459051 hasBestOaLocation W21554590512 @default.
• W2155459051 hasConcept C104317684 @default.
• W2155459051 hasConcept C12554922 @default.
• W2155459051 hasConcept C156719811 @default.
• W2155459051 hasConcept C164965036 @default.
• W2155459051 hasConcept C178790620 @default.
• W2155459051 hasConcept C185592680 @default.
• W2155459051 hasConcept C194830204 @default.
• W2155459051 hasConcept C2756471 @default.
• W2155459051 hasConcept C2777563447 @default.
• W2155459051 hasConcept C31254050 @default.
• W2155459051 hasConcept C49105822 @default.
• W2155459051 hasConcept C512185932 @default.
• W2155459051 hasConcept C552990157 @default.
• W2155459051 hasConcept C55493867 @default.
• W2155459051 hasConcept C82381507 @default.
• W2155459051 hasConcept C86803240 @default.
• W2155459051 hasConcept C88354546 @default.
• W2155459051 hasConceptScore W2155459051C104317684 @default.
• W2155459051 hasConceptScore W2155459051C12554922 @default.
• W2155459051 hasConceptScore W2155459051C156719811 @default.
• W2155459051 hasConceptScore W2155459051C164965036 @default.
• W2155459051 hasConceptScore W2155459051C178790620 @default.
• W2155459051 hasConceptScore W2155459051C185592680 @default.
• W2155459051 hasConceptScore W2155459051C194830204 @default.
• W2155459051 hasConceptScore W2155459051C2756471 @default.
• W2155459051 hasConceptScore W2155459051C2777563447 @default.
• W2155459051 hasConceptScore W2155459051C31254050 @default.
• W2155459051 hasConceptScore W2155459051C49105822 @default.
• W2155459051 hasConceptScore W2155459051C512185932 @default.
• W2155459051 hasConceptScore W2155459051C552990157 @default.
• W2155459051 hasConceptScore W2155459051C55493867 @default.
• W2155459051 hasConceptScore W2155459051C82381507 @default.

• next