FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155478762> ?p ?o ?g. }

• W2155478762 endingPage "118" @default.
• W2155478762 startingPage "108" @default.
• W2155478762 abstract "The first committed step in the biosynthesis of indole glucosinolates is the conversion of indole-3-acetaldoxime into an indole-3-S-alkyl-thiohydroximate. The initial step in this conversion is catalyzed by CYP83B1 in Arabidopsis (S. Bak, F.E. Tax, K.A. Feldmann, D.A. Galbraith, R. Feyereisen [2001] Plant Cell 13: 101-111). The knockout mutant of the CYP83B1 gene (rnt1-1) shows a strong auxin excess phenotype and are allelic to sur-2. CYP83A1 is the closest relative to CYP83B1 and shares 63% amino acid sequence identity. Although expression of CYP83A1 under control of its endogenous promoter in the rnt1-1 background does not prevent the auxin excess and indole glucosinolate deficit phenotype caused by the lack of the CYP83B1 gene, ectopic overexpression of CYP83A1 using a 35S promoter rescues the rnt1-1 phenotype. CYP83A1 and CYP83B1 heterologously expressed in yeast (Saccharomyces cerevisiae) cells show marked differences in their substrate specificity. Both enzymes convert indole-3-acetaldoxime to a thiohydroximate adduct in the presence of NADPH and a nucleophilic thiol donor. However, indole-3-acetaldoxime has a 50-fold higher affinity toward CYP83B1 than toward CYP83A1. Both enzymes also metabolize the phenylalanine- and tyrosine-derived aldoximes. Enzyme kinetic comparisons of CYP83A1 and CYP83B1 show that indole-3-acetaldoxime is the physiological substrate for CYP83B1 but not for CYP83A1. Instead, CYP83A1 catalyzes the initial conversion of aldoximes to thiohydroximates in the synthesis of glucosinolates not derived from tryptophan. The two closely related CYP83 subfamily members therefore are not redundant. The presence of putative auxin responsive cis-acting elements in the CYP83B1 promoter but not in the CYP83A1 promoter supports the suggestion that CYP83B1 has evolved to selectively metabolize a tryptophan-derived aldoxime intermediate shared with the pathway of auxin biosynthesis in Arabidopsis." @default.
• W2155478762 created "2016-06-24" @default.
• W2155478762 creator A5061684054 @default.
• W2155478762 creator A5068258357 @default.
• W2155478762 date "2001-09-01" @default.
• W2155478762 modified "2023-10-11" @default.
• W2155478762 title "The Involvement of Two P450 Enzymes, CYP83B1 and CYP83A1, in Auxin Homeostasis and Glucosinolate Biosynthesis" @default.
• W2155478762 cites W1501460217 @default.
• W2155478762 cites W1515404266 @default.
• W2155478762 cites W1561174912 @default.
• W2155478762 cites W1581549271 @default.
• W2155478762 cites W1605106021 @default.
• W2155478762 cites W1964322673 @default.
• W2155478762 cites W1964352120 @default.
• W2155478762 cites W1967456200 @default.
• W2155478762 cites W1971005452 @default.
• W2155478762 cites W1976738102 @default.
• W2155478762 cites W1976847926 @default.
• W2155478762 cites W1977650606 @default.
• W2155478762 cites W1988867650 @default.
• W2155478762 cites W1990206995 @default.
• W2155478762 cites W1990376824 @default.
• W2155478762 cites W1991177870 @default.
• W2155478762 cites W1993924390 @default.
• W2155478762 cites W1997145859 @default.
• W2155478762 cites W2000474786 @default.
• W2155478762 cites W2001807768 @default.
• W2155478762 cites W2001895804 @default.
• W2155478762 cites W2005975510 @default.
• W2155478762 cites W2006837524 @default.
• W2155478762 cites W2007253437 @default.
• W2155478762 cites W2024012002 @default.
• W2155478762 cites W2032585366 @default.
• W2155478762 cites W2048517444 @default.
• W2155478762 cites W2052315279 @default.
• W2155478762 cites W2055684244 @default.
• W2155478762 cites W2055914357 @default.
• W2155478762 cites W2057439433 @default.
• W2155478762 cites W2057547465 @default.
• W2155478762 cites W2069317038 @default.
• W2155478762 cites W2070734314 @default.
• W2155478762 cites W2072403742 @default.
• W2155478762 cites W2076719957 @default.
• W2155478762 cites W2090407854 @default.
• W2155478762 cites W2100945610 @default.
• W2155478762 cites W2104600672 @default.
• W2155478762 cites W2116441297 @default.
• W2155478762 cites W2118278450 @default.
• W2155478762 cites W2135674867 @default.
• W2155478762 cites W2150371370 @default.
• W2155478762 cites W2151544310 @default.
• W2155478762 cites W2171575877 @default.
• W2155478762 cites W2330412797 @default.
• W2155478762 cites W245936780 @default.
• W2155478762 cites W296793759 @default.
• W2155478762 cites W4238262529 @default.
• W2155478762 cites W994970986 @default.
• W2155478762 doi "https://doi.org/10.1104/pp.127.1.108" @default.
• W2155478762 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/117967" @default.
• W2155478762 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11553739" @default.
• W2155478762 hasPublicationYear "2001" @default.
• W2155478762 type Work @default.
• W2155478762 sameAs 2155478762 @default.
• W2155478762 citedByCount "228" @default.
• W2155478762 countsByYear W21554787622012 @default.
• W2155478762 countsByYear W21554787622013 @default.
• W2155478762 countsByYear W21554787622014 @default.
• W2155478762 countsByYear W21554787622015 @default.
• W2155478762 countsByYear W21554787622016 @default.
• W2155478762 countsByYear W21554787622017 @default.
• W2155478762 countsByYear W21554787622018 @default.
• W2155478762 countsByYear W21554787622019 @default.
• W2155478762 countsByYear W21554787622020 @default.
• W2155478762 countsByYear W21554787622021 @default.
• W2155478762 countsByYear W21554787622022 @default.
• W2155478762 countsByYear W21554787622023 @default.
• W2155478762 crossrefType "journal-article" @default.
• W2155478762 hasAuthorship W2155478762A5061684054 @default.
• W2155478762 hasAuthorship W2155478762A5068258357 @default.
• W2155478762 hasBestOaLocation W21554787621 @default.
• W2155478762 hasConcept C104317684 @default.
• W2155478762 hasConcept C143065580 @default.
• W2155478762 hasConcept C181199279 @default.
• W2155478762 hasConcept C192527728 @default.
• W2155478762 hasConcept C207778908 @default.
• W2155478762 hasConcept C2776747608 @default.
• W2155478762 hasConcept C2777576037 @default.
• W2155478762 hasConcept C2778164613 @default.
• W2155478762 hasConcept C2779222958 @default.
• W2155478762 hasConcept C2779491563 @default.
• W2155478762 hasConcept C553450214 @default.
• W2155478762 hasConcept C55493867 @default.
• W2155478762 hasConcept C6557445 @default.
• W2155478762 hasConcept C86803240 @default.
• W2155478762 hasConceptScore W2155478762C104317684 @default.
• W2155478762 hasConceptScore W2155478762C143065580 @default.
• W2155478762 hasConceptScore W2155478762C181199279 @default.
• W2155478762 hasConceptScore W2155478762C192527728 @default.

• next