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• W2155479819 abstract "Formins are a conserved family of actin assembly-promoting factors with essential and diverse biological roles. Most of our biochemical understanding of formin effects on actin dynamics is derived from studies using formin fragments. In addition, all structural information on formins has been limited to fragments. This has left open key questions about the structure, activity and regulation of intact formin proteins. Here, we isolated full-length mouse mDia1 (mDia1-FL) and found that it forms tightly autoinhibited dimers that can only be partially activated by RhoA. We solved the structure of autoinhibited mDia1-FL using electron microscopy and single particle analysis. Docking of crystal structures into the three dimensional reconstruction revealed that the fork-shaped N-terminal diaphanous inhibitory domain-coiled coil domain region hangs over the ring-shaped formin homology (FH)2 domain, suggesting that autoinhibition results from steric obstruction of actin binding. Deletion of the C-terminal diaphanous autoregulatory domain extended mDia1 structure and activated it for actin assembly. Using total internal reflection fluorescence microscopy, we observed that RhoA-activated mDia1-FL persistently accelerated filament elongation in the presence of profilin similar to mDia1 FH1-FH2 fragment. These observations validate the known activities of FH1-FH2 fragments as reflecting those of the intact molecule. Our results further suggest that mDia1-FL does not readily snap back into the autoinhibited conformation and dissociate from growing filament ends, and thus additional factors may be required to displace formins and restrict filament length. © 2012 Wiley Periodicals, Inc" @default.
• W2155479819 created "2016-06-24" @default.
• W2155479819 creator A5015312678 @default.
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• W2155479819 date "2012-06-01" @default.
• W2155479819 modified "2023-09-24" @default.
• W2155479819 title "Structure and activity of full-length formin mDia1" @default.
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• W2155479819 cites W1754544241 @default.
• W2155479819 cites W1838062783 @default.
• W2155479819 cites W1964716020 @default.
• W2155479819 cites W1972250746 @default.
• W2155479819 cites W1973870846 @default.
• W2155479819 cites W1974654719 @default.
• W2155479819 cites W1975287998 @default.
• W2155479819 cites W1988215817 @default.
• W2155479819 cites W1989309829 @default.
• W2155479819 cites W1990356394 @default.
• W2155479819 cites W1990850141 @default.
• W2155479819 cites W1995846053 @default.
• W2155479819 cites W1997228602 @default.
• W2155479819 cites W2008282204 @default.
• W2155479819 cites W2012587910 @default.
• W2155479819 cites W2012948303 @default.
• W2155479819 cites W2013121253 @default.
• W2155479819 cites W2019125570 @default.
• W2155479819 cites W2019261850 @default.
• W2155479819 cites W2019559746 @default.
• W2155479819 cites W2027866287 @default.
• W2155479819 cites W2028865261 @default.
• W2155479819 cites W2030222262 @default.
• W2155479819 cites W2033887263 @default.
• W2155479819 cites W2036535918 @default.
• W2155479819 cites W2037611941 @default.
• W2155479819 cites W2038657817 @default.
• W2155479819 cites W2044788731 @default.
• W2155479819 cites W2046903696 @default.
• W2155479819 cites W2047853156 @default.
• W2155479819 cites W2048826637 @default.
• W2155479819 cites W2048982317 @default.
• W2155479819 cites W2056713455 @default.
• W2155479819 cites W2057190824 @default.
• W2155479819 cites W2058655076 @default.
• W2155479819 cites W2059746552 @default.
• W2155479819 cites W2062852486 @default.
• W2155479819 cites W2064071063 @default.
• W2155479819 cites W2070119451 @default.
• W2155479819 cites W2070967303 @default.
• W2155479819 cites W2073786444 @default.
• W2155479819 cites W2081875880 @default.
• W2155479819 cites W2083523423 @default.
• W2155479819 cites W2085754596 @default.
• W2155479819 cites W2088071645 @default.
• W2155479819 cites W2094269474 @default.
• W2155479819 cites W2105087111 @default.
• W2155479819 cites W2105272775 @default.
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• W2155479819 cites W2114048476 @default.
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• W2155479819 cites W2123463281 @default.
• W2155479819 cites W2127735672 @default.
• W2155479819 cites W2131316350 @default.
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• W2155479819 cites W2139582633 @default.
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• W2155479819 cites W2142173778 @default.
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• W2155479819 doi "https://doi.org/10.1002/cm.21033" @default.
• W2155479819 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3416746" @default.
• W2155479819 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22605659" @default.
• W2155479819 hasPublicationYear "2012" @default.
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