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• W2155503541 endingPage "742" @default.
• W2155503541 startingPage "730" @default.
• W2155503541 abstract "Norwalk-like viruses (NLVs) are a diverse group of single-stranded, nonenveloped, positive-polarity RNA viruses and are the leading cause of epidemic acute gastroenteritis in the United States. In this study, the major capsid gene of Norwalk virus, the prototype NLV, has been cloned and expressed in mammalian cells using a Venezuelan equine encephalitis (VEE) replicon expression system. Upon infection of baby hamster kidney (BHK) cells with VEE replicon particles (VRPs), the Norwalk virus capsid proteins self-assemble to generate high titers of Norwalk virus-like particles (VLPs) that are morphologically and antigenically analogous to wild-type Norwalk virus. Mice inoculated subcutaneously with VRPs expressing the Norwalk virus capsid protein (VRP-NV1) developed systemic and mucosal immune responses to Norwalk VLPs, as well as heterotypic antibody responses to the major capsid protein from another genogroup I NLV strain (NCFL) isolated from a recent outbreak. A second Norwalk virus capsid clone (NV2) containing three amino acid codon mutations from the NV1 clone was also expressed using VEE replicons (VRP-NV2), but upon infection of BHK cells failed to confer VLP self-assembly. Mice inoculated with VRP-NV2 elicited reduced systemic and mucosal immune responses to Norwalk VLPs, demonstrating the importance and potential utility of endogenous VLP presentation for maximum immune induction. Inoculation with either VRP-NV1 or VRP-NV2 resulted in serum antibody responses far superior to the induction in mice dosed orally with VLPs that were prepared using the VEE-NV1 replicon construct, a regimen similar to current models for NLV vaccination. Expression of NLV VLPs in mammalian cells offers a powerful approach for the design of novel NLV vaccines, either alone or in combination with current vaccination models." @default.
• W2155503541 created "2016-06-24" @default.
• W2155503541 creator A5009039571 @default.
• W2155503541 creator A5010788467 @default.
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• W2155503541 date "2002-01-15" @default.
• W2155503541 modified "2023-09-27" @default.
• W2155503541 title "Systemic, Mucosal, and Heterotypic Immune Induction in Mice Inoculated with Venezuelan Equine Encephalitis Replicons Expressing Norwalk Virus-Like Particles" @default.
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• W2155503541 doi "https://doi.org/10.1128/jvi.76.2.730-742.2002" @default.
• W2155503541 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/136807" @default.
• W2155503541 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11752163" @default.
• W2155503541 hasPublicationYear "2002" @default.
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