Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155550943> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2155550943 endingPage "6332" @default.
- W2155550943 startingPage "6320" @default.
- W2155550943 abstract "Fluorescein is a complex fluorophore that can exist in one or more of four different prototropic forms (cation, neutral, dianion, and monoanion) depending on pH. In the pH range 6−10, only the dianion and monanion forms are important. In a previous article, we showed by steady-state fluorescein measurements that an excited fluorescein molecule displays excited-state proton transfer reactions which interconvert the monoanion and dianion forms. However, we found that these reactions can occur only in the presence of a suitable proton donor−acceptor buffer such as phosphate buffer. Assuming that, at 1 M phosphate buffer concentration, the excited-state proton exchange reaction of fluorescein rapidly equilibrates during the lifetime of fluorescein, we were able to fit quantitatively steady-state fluorescence intensity vs pH titration graphs to a relatively simple reaction model. In this article, we use nanosecond emission (decay time) methods to study the excited-state proton reactions of fluorescein in the pH range 6−10 and in the presence of a phosphate buffer concentration. Fluorescein is a challenging fluorophore for the study of excited-state proton reactions because of the strong overlap of the absorption and emission spectra of the monoanion and dianion forms of fluorescein. However by recording nanosecond emission graphs and using methods of analysis of high precision, we have been able to test kinetic mechanisms and evaluate the specific rate constants for the excited-state proton reactions as well as the lifetimes of the monoanion and dianion. Using these values for lifetimes and rate constants, we discuss the process of equilibration in the excited-state and derive expressions which allow us to predict how quickly the excited-state reactions can reach equilibrium. Moreover, we use the above kinetic and spectral parameters to calculate steady-state fluorescence intensity FS vs pH at 1 M phosphate buffer concentration and compare this theoretically calculated graph with the experimental graph." @default.
- W2155550943 created "2016-06-24" @default.
- W2155550943 creator A5009985683 @default.
- W2155550943 creator A5031294379 @default.
- W2155550943 creator A5057260712 @default.
- W2155550943 creator A5080118657 @default.
- W2155550943 date "2001-06-12" @default.
- W2155550943 modified "2023-09-26" @default.
- W2155550943 title "Fluorescein Excited-State Proton Exchange Reactions: Nanosecond Emission Kinetics and Correlation with Steady-State Fluorescence Intensity" @default.
- W2155550943 cites W1980007052 @default.
- W2155550943 cites W1981201790 @default.
- W2155550943 cites W2008878870 @default.
- W2155550943 cites W2024079171 @default.
- W2155550943 cites W2036958693 @default.
- W2155550943 cites W2053563780 @default.
- W2155550943 cites W2065263524 @default.
- W2155550943 cites W2084209753 @default.
- W2155550943 cites W2087070363 @default.
- W2155550943 cites W2088235037 @default.
- W2155550943 cites W2092617822 @default.
- W2155550943 cites W995681685 @default.
- W2155550943 doi "https://doi.org/10.1021/jp010372+" @default.
- W2155550943 hasPublicationYear "2001" @default.
- W2155550943 type Work @default.
- W2155550943 sameAs 2155550943 @default.
- W2155550943 citedByCount "83" @default.
- W2155550943 countsByYear W21555509432012 @default.
- W2155550943 countsByYear W21555509432013 @default.
- W2155550943 countsByYear W21555509432014 @default.
- W2155550943 countsByYear W21555509432015 @default.
- W2155550943 countsByYear W21555509432016 @default.
- W2155550943 countsByYear W21555509432017 @default.
- W2155550943 countsByYear W21555509432018 @default.
- W2155550943 countsByYear W21555509432019 @default.
- W2155550943 countsByYear W21555509432020 @default.
- W2155550943 countsByYear W21555509432021 @default.
- W2155550943 countsByYear W21555509432022 @default.
- W2155550943 countsByYear W21555509432023 @default.
- W2155550943 crossrefType "journal-article" @default.
- W2155550943 hasAuthorship W2155550943A5009985683 @default.
- W2155550943 hasAuthorship W2155550943A5031294379 @default.
- W2155550943 hasAuthorship W2155550943A5057260712 @default.
- W2155550943 hasAuthorship W2155550943A5080118657 @default.
- W2155550943 hasConcept C120665830 @default.
- W2155550943 hasConcept C121332964 @default.
- W2155550943 hasConcept C147789679 @default.
- W2155550943 hasConcept C148898269 @default.
- W2155550943 hasConcept C181500209 @default.
- W2155550943 hasConcept C184779094 @default.
- W2155550943 hasConcept C185544564 @default.
- W2155550943 hasConcept C185592680 @default.
- W2155550943 hasConcept C2778015030 @default.
- W2155550943 hasConcept C51141536 @default.
- W2155550943 hasConcept C520434653 @default.
- W2155550943 hasConcept C54516573 @default.
- W2155550943 hasConcept C62520636 @default.
- W2155550943 hasConcept C75473681 @default.
- W2155550943 hasConcept C8171440 @default.
- W2155550943 hasConcept C91881484 @default.
- W2155550943 hasConcept C93038891 @default.
- W2155550943 hasConceptScore W2155550943C120665830 @default.
- W2155550943 hasConceptScore W2155550943C121332964 @default.
- W2155550943 hasConceptScore W2155550943C147789679 @default.
- W2155550943 hasConceptScore W2155550943C148898269 @default.
- W2155550943 hasConceptScore W2155550943C181500209 @default.
- W2155550943 hasConceptScore W2155550943C184779094 @default.
- W2155550943 hasConceptScore W2155550943C185544564 @default.
- W2155550943 hasConceptScore W2155550943C185592680 @default.
- W2155550943 hasConceptScore W2155550943C2778015030 @default.
- W2155550943 hasConceptScore W2155550943C51141536 @default.
- W2155550943 hasConceptScore W2155550943C520434653 @default.
- W2155550943 hasConceptScore W2155550943C54516573 @default.
- W2155550943 hasConceptScore W2155550943C62520636 @default.
- W2155550943 hasConceptScore W2155550943C75473681 @default.
- W2155550943 hasConceptScore W2155550943C8171440 @default.
- W2155550943 hasConceptScore W2155550943C91881484 @default.
- W2155550943 hasConceptScore W2155550943C93038891 @default.
- W2155550943 hasIssue "26" @default.
- W2155550943 hasLocation W21555509431 @default.
- W2155550943 hasOpenAccess W2155550943 @default.
- W2155550943 hasPrimaryLocation W21555509431 @default.
- W2155550943 hasRelatedWork W1997729587 @default.
- W2155550943 hasRelatedWork W2017674624 @default.
- W2155550943 hasRelatedWork W2025058865 @default.
- W2155550943 hasRelatedWork W2031675822 @default.
- W2155550943 hasRelatedWork W2044741007 @default.
- W2155550943 hasRelatedWork W2048628899 @default.
- W2155550943 hasRelatedWork W2052940040 @default.
- W2155550943 hasRelatedWork W2167089189 @default.
- W2155550943 hasRelatedWork W2167481674 @default.
- W2155550943 hasRelatedWork W4247028702 @default.
- W2155550943 hasVolume "105" @default.
- W2155550943 isParatext "false" @default.
- W2155550943 isRetracted "false" @default.
- W2155550943 magId "2155550943" @default.
- W2155550943 workType "article" @default.