Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155614325> ?p ?o ?g. }
- W2155614325 endingPage "15408" @default.
- W2155614325 startingPage "15397" @default.
- W2155614325 abstract "The minichromosome maintenance (MCM) proteins, together with the origin recognition complex (ORC) proteins and Cdc6, play an essential role in eukaryotic DNA replication through the formation of a pre-replication complex at origins of replication. We used a yeast two-hybrid screen to identify MCM2-interacting proteins. One of the proteins we identified is identical to the ORC1-interacting protein termed HBO1. HBO1 belongs to the MYST family, characterized by a highly conserved C2HC zinc finger and a putative histone acetyltransferase domain. Biochemical studies confirmed the interaction between MCM2 and HBO1 in vitro and in vivo. An N-terminal domain of MCM2 is necessary for binding to HBO1, and a C2HC zinc finger of HBO1 is essential for binding to MCM2. A reverse yeast two-hybrid selection was performed to isolate an allele of MCM2 that is defective for interaction with HBO1; this allele was then used to isolate a suppressor mutant of HBO1 that restores the interaction with the mutant MCM2. This suppressor mutation was located in the HBO1 zinc finger. Taken together, these findings strongly suggest that the interaction between MCM2 and HBO1 is direct and mediated by the C2HC zinc finger of HBO1. The biochemical and genetic interactions of MYST family protein HBO1 with two components of the replication apparatus, MCM2 and ORC1, suggest that HBO1-associated HAT activity may play a direct role in the process of DNA replication." @default.
- W2155614325 created "2016-06-24" @default.
- W2155614325 creator A5016251947 @default.
- W2155614325 creator A5043654825 @default.
- W2155614325 creator A5048419710 @default.
- W2155614325 creator A5068032908 @default.
- W2155614325 date "2001-01-01" @default.
- W2155614325 modified "2023-10-14" @default.
- W2155614325 title "Replication Factors MCM2 and ORC1 Interact with the Histone Acetyltransferase HBO1" @default.
- W2155614325 cites W10054795 @default.
- W2155614325 cites W1266943776 @default.
- W2155614325 cites W1539177644 @default.
- W2155614325 cites W1579420753 @default.
- W2155614325 cites W1601514415 @default.
- W2155614325 cites W1913652972 @default.
- W2155614325 cites W1964439976 @default.
- W2155614325 cites W1968074226 @default.
- W2155614325 cites W1973311604 @default.
- W2155614325 cites W1975593876 @default.
- W2155614325 cites W1980492780 @default.
- W2155614325 cites W1980637163 @default.
- W2155614325 cites W1985973665 @default.
- W2155614325 cites W1986648879 @default.
- W2155614325 cites W1987861824 @default.
- W2155614325 cites W1990299164 @default.
- W2155614325 cites W1991762784 @default.
- W2155614325 cites W1992661805 @default.
- W2155614325 cites W2003959153 @default.
- W2155614325 cites W2011726989 @default.
- W2155614325 cites W2012931476 @default.
- W2155614325 cites W2014463884 @default.
- W2155614325 cites W2014720884 @default.
- W2155614325 cites W2015472477 @default.
- W2155614325 cites W2017675402 @default.
- W2155614325 cites W2022094921 @default.
- W2155614325 cites W2022537514 @default.
- W2155614325 cites W2025011614 @default.
- W2155614325 cites W2028746163 @default.
- W2155614325 cites W2029903254 @default.
- W2155614325 cites W2034331383 @default.
- W2155614325 cites W2040807564 @default.
- W2155614325 cites W2044651119 @default.
- W2155614325 cites W2045879436 @default.
- W2155614325 cites W2052093902 @default.
- W2155614325 cites W2052259420 @default.
- W2155614325 cites W2052632710 @default.
- W2155614325 cites W2052881693 @default.
- W2155614325 cites W2056369410 @default.
- W2155614325 cites W2056590571 @default.
- W2155614325 cites W2057594508 @default.
- W2155614325 cites W2059594509 @default.
- W2155614325 cites W2059906457 @default.
- W2155614325 cites W2064719780 @default.
- W2155614325 cites W2081099987 @default.
- W2155614325 cites W2088729221 @default.
- W2155614325 cites W2089836142 @default.
- W2155614325 cites W2099416436 @default.
- W2155614325 cites W2107372552 @default.
- W2155614325 cites W2108606404 @default.
- W2155614325 cites W2116808406 @default.
- W2155614325 cites W2126909443 @default.
- W2155614325 cites W2129781454 @default.
- W2155614325 cites W2144892831 @default.
- W2155614325 cites W2149799872 @default.
- W2155614325 cites W2159206340 @default.
- W2155614325 cites W2159268545 @default.
- W2155614325 cites W2162540931 @default.
- W2155614325 cites W2168215139 @default.
- W2155614325 cites W2170085122 @default.
- W2155614325 cites W2182220639 @default.
- W2155614325 cites W2271067653 @default.
- W2155614325 cites W4242753923 @default.
- W2155614325 cites W4253422011 @default.
- W2155614325 cites W4300667300 @default.
- W2155614325 doi "https://doi.org/10.1074/jbc.m011556200" @default.
- W2155614325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11278932" @default.
- W2155614325 hasPublicationYear "2001" @default.
- W2155614325 type Work @default.
- W2155614325 sameAs 2155614325 @default.
- W2155614325 citedByCount "186" @default.
- W2155614325 countsByYear W21556143252012 @default.
- W2155614325 countsByYear W21556143252013 @default.
- W2155614325 countsByYear W21556143252014 @default.
- W2155614325 countsByYear W21556143252015 @default.
- W2155614325 countsByYear W21556143252016 @default.
- W2155614325 countsByYear W21556143252017 @default.
- W2155614325 countsByYear W21556143252018 @default.
- W2155614325 countsByYear W21556143252019 @default.
- W2155614325 countsByYear W21556143252020 @default.
- W2155614325 countsByYear W21556143252021 @default.
- W2155614325 countsByYear W21556143252022 @default.
- W2155614325 countsByYear W21556143252023 @default.
- W2155614325 crossrefType "journal-article" @default.
- W2155614325 hasAuthorship W2155614325A5016251947 @default.
- W2155614325 hasAuthorship W2155614325A5043654825 @default.
- W2155614325 hasAuthorship W2155614325A5048419710 @default.
- W2155614325 hasAuthorship W2155614325A5068032908 @default.
- W2155614325 hasBestOaLocation W21556143251 @default.