FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155656827> ?p ?o ?g. }

• W2155656827 endingPage "463" @default.
• W2155656827 startingPage "449" @default.
• W2155656827 abstract "The sperm anterior head plasma membrane (APM) is the site where sperm first bind to the zona pellucida (ZP). This binding reaches the maximum following the sperm capacitation process. To gain a better understanding of the sperm-ZP binding mechanisms, we compared protein profiles obtained from mass spectrometry of APM vesicles isolated from non-capacitated and capacitated sperm. The results revealed that ZP-binding proteins were the most abundant group of proteins, with a number of them showing increased levels in capacitated sperm. Blue native gel electrophoresis and far-western blotting revealed presence of high molecular weight (HMW) protein complexes in APM vesicles of both non-capacitated and capacitated sperm, but the complexes (∼750-1300 kDa) from capacitated sperm possessed much higher binding capacity to pig ZP3 glycoprotein. Proteomic analyses indicated that a number of proteins known for their acrosome localization, including zonadhesin, proacrosin/acrosin and ACRBP, were components of capacitated APM HMW complexes, with zonadhesin being the most enriched protein. Our immunofluorescence results further demonstrated that a fraction of these acrosomal proteins was transported to the surface of live acrosome-intact sperm during capacitation. Co-immunoprecipitation indicated that zonadhesin, proacrosin/acrosin and ACRBP interacted with each other and they may traffic as a complex from the acrosome to the sperm surface. Finally, the significance of zonadhesin in the binding of APM HMW complexes to pig ZP3 was demonstrated; the binding ability was decreased following treatment of the complexes with anti-zonadhesin antibody. Our results suggested that acrosomal proteins, especially zonadhesin, played roles in the initial sperm-ZP binding during capacitation." @default.
• W2155656827 created "2016-06-24" @default.
• W2155656827 creator A5002944057 @default.
• W2155656827 creator A5004273849 @default.
• W2155656827 creator A5017199339 @default.
• W2155656827 creator A5029163552 @default.
• W2155656827 creator A5039255378 @default.
• W2155656827 creator A5039388056 @default.
• W2155656827 creator A5043585430 @default.
• W2155656827 creator A5046449541 @default.
• W2155656827 creator A5053345353 @default.
• W2155656827 creator A5053623016 @default.
• W2155656827 creator A5067380502 @default.
• W2155656827 creator A5073896166 @default.
• W2155656827 creator A5082961597 @default.
• W2155656827 creator A5088651676 @default.
• W2155656827 date "2014-10-28" @default.
• W2155656827 modified "2023-09-25" @default.
• W2155656827 title "Proteomic Characterization of Pig Sperm Anterior Head Plasma Membrane Reveals Roles of Acrosomal Proteins in ZP3 Binding" @default.
• W2155656827 cites W107747064 @default.
• W2155656827 cites W1529389226 @default.
• W2155656827 cites W1543901357 @default.
• W2155656827 cites W1601998050 @default.
• W2155656827 cites W1896539835 @default.
• W2155656827 cites W1946891264 @default.
• W2155656827 cites W1968274873 @default.
• W2155656827 cites W1968843575 @default.
• W2155656827 cites W1971496343 @default.
• W2155656827 cites W1972192342 @default.
• W2155656827 cites W1972754432 @default.
• W2155656827 cites W1972895952 @default.
• W2155656827 cites W1973006797 @default.
• W2155656827 cites W1975534833 @default.
• W2155656827 cites W1975840953 @default.
• W2155656827 cites W1977656954 @default.
• W2155656827 cites W1979499846 @default.
• W2155656827 cites W1981117876 @default.
• W2155656827 cites W1991953616 @default.
• W2155656827 cites W1994574697 @default.
• W2155656827 cites W2000092397 @default.
• W2155656827 cites W2000202551 @default.
• W2155656827 cites W2005336167 @default.
• W2155656827 cites W2028520495 @default.
• W2155656827 cites W2030596152 @default.
• W2155656827 cites W2032040517 @default.
• W2155656827 cites W2042317997 @default.
• W2155656827 cites W2048020436 @default.
• W2155656827 cites W2053072251 @default.
• W2155656827 cites W2054575651 @default.
• W2155656827 cites W2057733697 @default.
• W2155656827 cites W2059766761 @default.
• W2155656827 cites W2061792951 @default.
• W2155656827 cites W2063434891 @default.
• W2155656827 cites W2065296043 @default.
• W2155656827 cites W2065520693 @default.
• W2155656827 cites W2065984876 @default.
• W2155656827 cites W2068119233 @default.
• W2155656827 cites W2070468337 @default.
• W2155656827 cites W2071905627 @default.
• W2155656827 cites W2072169540 @default.
• W2155656827 cites W2075879058 @default.
• W2155656827 cites W2079802547 @default.
• W2155656827 cites W2084140901 @default.
• W2155656827 cites W2087119080 @default.
• W2155656827 cites W2087378494 @default.
• W2155656827 cites W2093402385 @default.
• W2155656827 cites W2093409717 @default.
• W2155656827 cites W2098294237 @default.
• W2155656827 cites W2099934017 @default.
• W2155656827 cites W2100837269 @default.
• W2155656827 cites W2102504274 @default.
• W2155656827 cites W2110221276 @default.
• W2155656827 cites W2111645881 @default.
• W2155656827 cites W2115236083 @default.
• W2155656827 cites W2117135978 @default.
• W2155656827 cites W2118797808 @default.
• W2155656827 cites W2124984913 @default.
• W2155656827 cites W2127489545 @default.
• W2155656827 cites W2129841410 @default.
• W2155656827 cites W2134246284 @default.
• W2155656827 cites W2139444606 @default.
• W2155656827 cites W2140465002 @default.
• W2155656827 cites W2146303492 @default.
• W2155656827 cites W2149475559 @default.
• W2155656827 cites W2152046517 @default.
• W2155656827 cites W2154938203 @default.
• W2155656827 cites W2156369781 @default.
• W2155656827 cites W2167049138 @default.
• W2155656827 cites W2169544045 @default.
• W2155656827 cites W2171681751 @default.
• W2155656827 cites W2171954405 @default.
• W2155656827 cites W4295202618 @default.
• W2155656827 doi "https://doi.org/10.1002/jcp.24728" @default.
• W2155656827 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25078272" @default.
• W2155656827 hasPublicationYear "2014" @default.
• W2155656827 type Work @default.
• W2155656827 sameAs 2155656827 @default.
• W2155656827 citedByCount "33" @default.

• next