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- W2155666162 abstract "Background: Antiretroviral drugs (ARV) are being provided by Govt. of India since 2004 under 3 by 5 initiative led by WHO-UNAIDS. Despite wide spread use of ARV, little information is available on the prevalence of transmitted resistance-associated mutations in HIV-1, particularly from north India. The present study was designed for molecular characterization of HIV-1 and identification of ARV resistance- associated mutations and naturally occurring genotypic variation in reverse transcriptase (RT) and protease region of pol gene of HIV-1. Methods: Seventy ARV drug-naïve HIV-1 sero-reactive patients, newly registered in ART clinic of Lok Nayak Hospital, were enrolled in the study after obtaining written informed consent. Plasma HIV-1 RNA levels were estimated by Amplicor HIV-1 Monitor test V 1.5 (Roche Diagnostics). HIV-1 pol gene, enconsisting protease region (codons;1-99) and RT region (codons;1-232 to 1-239) was reverse transcribed, followed by nested PCR with self designed primers. The PCR product was sequenced with an ABI PRISM 3100 genetic analyzer system. Results: The median HIV-1 RNA levels were log10 4.6 copies/ml (IQR; 4.2-5.3). All patients were found to be infected with HIV-1 subtype C. Only one (1.4%) study sequence harbored resistance-associated mutations in RT region, showing NRTI resistance mutation (M184I). No mutation at major resistance positions in protease region was detected. High rate of polymorphisms were observed at codons; 35, 39, 40, 48, 60, 121, 122, 123, 135, 166, 173, 174, 177, 185, 200, 204, 206, 207, 208, 211, 216, 217, 233 & 239 in RT gene and 12, 15, 18, 19, 36, 38, 41, 63, 69, 70, 89 & 93 in protease gene. Conclusion: The overall prevalence of transmitted resistance-associated mutations was found to be 1.4%, lower than the alert cut off of 5% defined by WHO. HIV-1 subtype C exhibited enormous polymorphism in RT and protease regions of pol gene as compared to subtype B. Abstracts for SupplementInternational Journal of Infectious DiseasesVol. 14Preview Full-Text PDF Open Archive" @default.
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- W2155666162 date "2010-03-01" @default.
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- W2155666162 title "Antiretroviral resistance-associated mutations and genomic diversity in reverse transcriptase and protease gene among drug-naïve patients" @default.
- W2155666162 doi "https://doi.org/10.1016/j.ijid.2010.02.2039" @default.
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