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• W2155705877 abstract "In this paper we present a novel expectation-maximization algorithm for inference of alternative splicing isoform frequencies from high-throughput transcriptome sequencing (RNA-Seq) data. Our algorithm exploits disambiguation information provided by the distribution of insert sizes generated during sequencing library preparation, and takes advantage of base quality scores, strand and read pairing information if available. Empirical experiments on synthetic datasets show that the algorithm significantly outperforms existing methods of isoform and gene expression level estimation from RNA-Seq data. The Java implementation of IsoEM is available at http://dna.engr.uconn.edu/software/IsoEM/." @default.
• W2155705877 created "2016-06-24" @default.
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• W2155705877 date "2010-01-01" @default.
• W2155705877 modified "2023-10-17" @default.
• W2155705877 title "Estimation of Alternative Splicing isoform Frequencies from RNA-Seq Data" @default.
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• W2155705877 doi "https://doi.org/10.1007/978-3-642-15294-8_17" @default.
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