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• W2155809353 abstract "Streptomyces clavuligerus ATCC 27064 is unable to use glucose but has genes for a glucose permease ( glcP ) and a glucose kinase ( glkA ). Transformation of S. clavuligerus 27064 with the Streptomyces coelicolor glcP1 gene with its own promoter results in a strain able to grow on glucose. The glcP gene of S. clavuligerus encodes a 475 amino acid glucose permease with 12 transmembrane segments. GlcP is a functional protein when expressed from the S. coelicolor glcP1 promoter and complements two different glucose transport-negative Escherichia coli mutants. Transcription studies indicate that the glcP promoter is very weak and does not allow growth on glucose. These results suggest that S. clavuligerus initially contained a functional glucose permease gene, like most other Streptomyces species, and lost the expression of this gene by adaptation to glucose-poor habitats." @default.
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• W2155809353 date "2010-05-01" @default.
• W2155809353 modified "2023-10-03" @default.
• W2155809353 title "The enigmatic lack of glucose utilization in Streptomyces clavuligerus is due to inefficient expression of the glucose permease gene" @default.
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• W2155809353 doi "https://doi.org/10.1099/mic.0.035840-0" @default.
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