Matches in SemOpenAlex for { <https://semopenalex.org/work/W2155890498> ?p ?o ?g. }
- W2155890498 endingPage "10" @default.
- W2155890498 startingPage "1" @default.
- W2155890498 abstract "1. Azotobacter vinelandii cells were ruptured by using a French pressure cell, by lysozyme treatment, or by osmotic shock. The rates of sedimentation of nitrogenase from these extracts were compared and related to the rates of sedimentation of marker enzymes. No evidence was found for the so-called particulate nitrogenase or an interaction between nitrogenase and cytoplasmic membrane vesicles. Nitrogenase sediments as a complex and the rate of sedimentation is comparable with that of pyruvate dehydrogenase complex. 2. The oxygen stability of nitrogenase from Azotobacter is not caused by the presence of cytoplasmic membranes in extracts but by a complexation of the nitrogenase components with an Fe-S protein. Recombination experiments showed that maximum stabilization against oxygen was only obtained at certain ratios of the Fe-S-protein and nitrogenase complex. It is proposed that the switch-off state in Azotobacter cells is caused by the oxidation of flavodoxin hydroquinone rather than by a reversible inactivation of the nitrogenase. 3. A membrane-bound NAD(P)H-flavodoxin oxidoreductase was detected. Evidence was obtained that flavodoxin might also be membrane-bound. 4. A proposal is given for electron donation to nitrogenase in Azotobacter. In this proposal the generation of reducing equivalents for nitrogenase is localized in the cytoplasmic membrane and mediated by the NADH-flavodoxin oxidoreductase. The pH gradient which is generated by membrane energization, is used to reduce flavodoxin to its hydroquinone form in order to achieve a redox potential low enough to reduce nitrogenase." @default.
- W2155890498 created "2016-06-24" @default.
- W2155890498 creator A5002313372 @default.
- W2155890498 creator A5031172878 @default.
- W2155890498 date "1977-07-01" @default.
- W2155890498 modified "2023-09-24" @default.
- W2155890498 title "Involvement of the cytoplasmic membrane in nitrogen fixation by azotobacter vinelandii" @default.
- W2155890498 cites W1007161724 @default.
- W2155890498 cites W1497676488 @default.
- W2155890498 cites W1512041247 @default.
- W2155890498 cites W1594978818 @default.
- W2155890498 cites W1787794677 @default.
- W2155890498 cites W1864809073 @default.
- W2155890498 cites W1866602906 @default.
- W2155890498 cites W1890757170 @default.
- W2155890498 cites W1919876035 @default.
- W2155890498 cites W1927627186 @default.
- W2155890498 cites W1965219901 @default.
- W2155890498 cites W1965246707 @default.
- W2155890498 cites W1967298352 @default.
- W2155890498 cites W1975710321 @default.
- W2155890498 cites W1983387657 @default.
- W2155890498 cites W2003328537 @default.
- W2155890498 cites W2013656249 @default.
- W2155890498 cites W2018307445 @default.
- W2155890498 cites W2030043066 @default.
- W2155890498 cites W2030545388 @default.
- W2155890498 cites W2044243926 @default.
- W2155890498 cites W2046376265 @default.
- W2155890498 cites W2053239712 @default.
- W2155890498 cites W2056090110 @default.
- W2155890498 cites W2056473486 @default.
- W2155890498 cites W2062261415 @default.
- W2155890498 cites W2082341001 @default.
- W2155890498 cites W2085243286 @default.
- W2155890498 cites W2088957632 @default.
- W2155890498 cites W2102582319 @default.
- W2155890498 cites W2168157119 @default.
- W2155890498 cites W21704270 @default.
- W2155890498 cites W2208316638 @default.
- W2155890498 cites W2472803516 @default.
- W2155890498 cites W2615281588 @default.
- W2155890498 cites W291421590 @default.
- W2155890498 doi "https://doi.org/10.1111/j.1432-1033.1977.tb11634.x" @default.
- W2155890498 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/908330" @default.
- W2155890498 hasPublicationYear "1977" @default.
- W2155890498 type Work @default.
- W2155890498 sameAs 2155890498 @default.
- W2155890498 citedByCount "83" @default.
- W2155890498 countsByYear W21558904982013 @default.
- W2155890498 countsByYear W21558904982015 @default.
- W2155890498 countsByYear W21558904982017 @default.
- W2155890498 crossrefType "journal-article" @default.
- W2155890498 hasAuthorship W2155890498A5002313372 @default.
- W2155890498 hasAuthorship W2155890498A5031172878 @default.
- W2155890498 hasBestOaLocation W21558904981 @default.
- W2155890498 hasConcept C107563273 @default.
- W2155890498 hasConcept C134621786 @default.
- W2155890498 hasConcept C141280058 @default.
- W2155890498 hasConcept C178790620 @default.
- W2155890498 hasConcept C181199279 @default.
- W2155890498 hasConcept C181440489 @default.
- W2155890498 hasConcept C185592680 @default.
- W2155890498 hasConcept C2776159390 @default.
- W2155890498 hasConcept C2776740245 @default.
- W2155890498 hasConcept C2779268744 @default.
- W2155890498 hasConcept C523546767 @default.
- W2155890498 hasConcept C537208039 @default.
- W2155890498 hasConcept C54355233 @default.
- W2155890498 hasConcept C55493867 @default.
- W2155890498 hasConcept C86803240 @default.
- W2155890498 hasConceptScore W2155890498C107563273 @default.
- W2155890498 hasConceptScore W2155890498C134621786 @default.
- W2155890498 hasConceptScore W2155890498C141280058 @default.
- W2155890498 hasConceptScore W2155890498C178790620 @default.
- W2155890498 hasConceptScore W2155890498C181199279 @default.
- W2155890498 hasConceptScore W2155890498C181440489 @default.
- W2155890498 hasConceptScore W2155890498C185592680 @default.
- W2155890498 hasConceptScore W2155890498C2776159390 @default.
- W2155890498 hasConceptScore W2155890498C2776740245 @default.
- W2155890498 hasConceptScore W2155890498C2779268744 @default.
- W2155890498 hasConceptScore W2155890498C523546767 @default.
- W2155890498 hasConceptScore W2155890498C537208039 @default.
- W2155890498 hasConceptScore W2155890498C54355233 @default.
- W2155890498 hasConceptScore W2155890498C55493867 @default.
- W2155890498 hasConceptScore W2155890498C86803240 @default.
- W2155890498 hasIssue "1" @default.
- W2155890498 hasLocation W21558904981 @default.
- W2155890498 hasLocation W21558904982 @default.
- W2155890498 hasOpenAccess W2155890498 @default.
- W2155890498 hasPrimaryLocation W21558904981 @default.
- W2155890498 hasRelatedWork W1583887726 @default.
- W2155890498 hasRelatedWork W1759578540 @default.
- W2155890498 hasRelatedWork W1994969373 @default.
- W2155890498 hasRelatedWork W2046376265 @default.
- W2155890498 hasRelatedWork W2155890498 @default.
- W2155890498 hasRelatedWork W2202821626 @default.
- W2155890498 hasRelatedWork W2315746299 @default.