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- W2155941870 abstract "Fluorescence microscopic analysis of newly replicated DNA has revealed discrete granular sites of replication (RS). The average size and number of replication sites from early to mid S-phase suggest that each RS contains numerous replicons clustered together. We are using fluorescence laser scanning confocal microscopy in conjunction with multidimensional image analysis to gain more precise information about RS and their spatial-temporal dynamics. Using a newly improved imaging segmentation program, we report an average of ∼1,100 RS after a 5-min pulse labeling of 3T3 mouse fibroblast cells in early S-phase. Pulse-chase-pulse double labeling experiments reveal that RS take ∼45 min to complete replication. Appropriate calculations suggest that each RS contains an average of 1 mbp of DNA or ∼6 average-sized replicons. Double pulse–double chase experiments demonstrate that the DNA sequences replicated at individual RS are precisely maintained temporally and spatially as the cell progresses through the cell cycle and into subsequent generations. By labeling replicated DNA at the G1/S borders for two consecutive cell generations, we show that the DNA synthesized at early S-phase is replicated at the same time and sites in the next round of replication." @default.
- W2155941870 created "2016-06-24" @default.
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- W2155941870 date "1998-12-14" @default.
- W2155941870 modified "2023-10-11" @default.
- W2155941870 title "Spatial and Temporal Dynamics of DNA Replication Sites in Mammalian Cells" @default.
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- W2155941870 doi "https://doi.org/10.1083/jcb.143.6.1415" @default.
- W2155941870 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2132991" @default.
- W2155941870 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9852140" @default.
- W2155941870 hasPublicationYear "1998" @default.
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