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- W215596806 abstract "Microbial life in extreme environments has been studied primarily for their metabolic activities. Very few commercial or industrial applications have been known from these systems. In this study, the metabolic pathways and properties of bacteria from a haloalkaliphilic environment of Soap Lake, Washington were employed in two research applications related to energy production. In the first study, the bacterial cultures that were known to reduce iron (III) were used in a Microbial Fuel Cell (MFC) reactor. Iron reducing bacteria have shown to shunt their electrons on to the surface of a carbon electrode of an MFC, and the electrons can be harvested to produce current. The research showed that haloalkaliphilic bacteria colonized the electrode surface efficiently and produced current densities up to 12.5 mA/m. The open circuit voltage was constant over several days reaching up to ~1V. Molecular characterization of 16S rRNA gene and RFLP analysis showed that the bacterium responsible was a single species with 97% 16S rRNA gene similarity to Halanaerobium sp. The second study focused on applying the fermentative mechanism of the bacterial cultures from Soap Lake. Bacteria were found to use glycerol as the substrate and ferment it to ethanol. Glycerol is a major by-product of biodiesel industry and therefore bacterial fermentation of this glycerol to ethanol would help to manage the waste as well as produce a value-added product. Analysis of glycerol and ethanol concentrations showed a conversion rate of about 2.83%. Several strategies were adopted to identify the bacterial species involved in the fermentation process. Results from RFLP and 16S rRNA gene analysis indicated that this bacterium showed 97% gene similarity to Halanaerobium sp." @default.
- W215596806 created "2016-06-24" @default.
- W215596806 creator A5072279814 @default.
- W215596806 date "2009-01-01" @default.
- W215596806 modified "2023-09-26" @default.
- W215596806 title "Electricity generation and ethanol production using iron-reducing, haloalkaliphilic bacteria" @default.
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