Matches in SemOpenAlex for { <https://semopenalex.org/work/W2156020419> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2156020419 endingPage "865" @default.
- W2156020419 startingPage "864" @default.
- W2156020419 abstract "Double emulsion solvent evaporation is a widely used method to prepare poly(dl-lactide-co-glycolide) (PLGA) microparticles encapsulating plasmid DNA. There are inherent problems associated with preparing plasmid DNA in this form, in particular the DNA is liable to degrade during manufacture and the resulting powder has low encapsulation efficiencies. This study compares the use of two buffers, 0.1 M NaHCO3 and 0.07 M Na2HPO4 and the effect these have on the encapsulation efficiency and other critical parameters associated with these encapsulated DNA materials. Both buffers preserved the conformation of the original plasmid DNA during the homogenization process, but those made with 0.07 M Na2HPO4 had higher encapsulation efficiencies, as well as smaller diameters, compared with those made with 0.1 M NaHCO3 (encapsulation efficiencies of 40.72–45.65%, and mean volume diameters of 2.96–4.45 μm). Buffers with a range of pH from 5 to 12 were investigated, and it was demonstrated that pH 9 was the point at which the highest amount of supercoiled DNA was balanced with the highest encapsulation efficiency. To simulate in vitro release, it was shown that microparticles made with 0.07 M Na2HPO4 had lower DNA release rates than those made with 0.1 M NaHCO3. These results demonstrate that the use of different buffers can aid in retaining the conformation of plasmid DNA, and can also modulate the amount of DNA encapsulated and the release profiles of microparticles." @default.
- W2156020419 created "2016-06-24" @default.
- W2156020419 creator A5026909200 @default.
- W2156020419 date "2006-01-01" @default.
- W2156020419 modified "2023-09-27" @default.
- W2156020419 title "Controlled release of DNA vaccines: Does in vitro release profile correlate with immune response?" @default.
- W2156020419 cites W1972243488 @default.
- W2156020419 cites W1998429819 @default.
- W2156020419 cites W2044352403 @default.
- W2156020419 doi "https://doi.org/10.1016/j.mehy.2005.11.031" @default.
- W2156020419 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16406686" @default.
- W2156020419 hasPublicationYear "2006" @default.
- W2156020419 type Work @default.
- W2156020419 sameAs 2156020419 @default.
- W2156020419 citedByCount "2" @default.
- W2156020419 countsByYear W21560204192013 @default.
- W2156020419 crossrefType "journal-article" @default.
- W2156020419 hasAuthorship W2156020419A5026909200 @default.
- W2156020419 hasConcept C12554922 @default.
- W2156020419 hasConcept C127413603 @default.
- W2156020419 hasConcept C130217890 @default.
- W2156020419 hasConcept C163588942 @default.
- W2156020419 hasConcept C171250308 @default.
- W2156020419 hasConcept C185592680 @default.
- W2156020419 hasConcept C18903297 @default.
- W2156020419 hasConcept C192562407 @default.
- W2156020419 hasConcept C202751555 @default.
- W2156020419 hasConcept C22744801 @default.
- W2156020419 hasConcept C2778123984 @default.
- W2156020419 hasConcept C2778722038 @default.
- W2156020419 hasConcept C2780165375 @default.
- W2156020419 hasConcept C42360764 @default.
- W2156020419 hasConcept C43617362 @default.
- W2156020419 hasConcept C552990157 @default.
- W2156020419 hasConcept C55493867 @default.
- W2156020419 hasConcept C86803240 @default.
- W2156020419 hasConceptScore W2156020419C12554922 @default.
- W2156020419 hasConceptScore W2156020419C127413603 @default.
- W2156020419 hasConceptScore W2156020419C130217890 @default.
- W2156020419 hasConceptScore W2156020419C163588942 @default.
- W2156020419 hasConceptScore W2156020419C171250308 @default.
- W2156020419 hasConceptScore W2156020419C185592680 @default.
- W2156020419 hasConceptScore W2156020419C18903297 @default.
- W2156020419 hasConceptScore W2156020419C192562407 @default.
- W2156020419 hasConceptScore W2156020419C202751555 @default.
- W2156020419 hasConceptScore W2156020419C22744801 @default.
- W2156020419 hasConceptScore W2156020419C2778123984 @default.
- W2156020419 hasConceptScore W2156020419C2778722038 @default.
- W2156020419 hasConceptScore W2156020419C2780165375 @default.
- W2156020419 hasConceptScore W2156020419C42360764 @default.
- W2156020419 hasConceptScore W2156020419C43617362 @default.
- W2156020419 hasConceptScore W2156020419C552990157 @default.
- W2156020419 hasConceptScore W2156020419C55493867 @default.
- W2156020419 hasConceptScore W2156020419C86803240 @default.
- W2156020419 hasIssue "4" @default.
- W2156020419 hasLocation W21560204191 @default.
- W2156020419 hasLocation W21560204192 @default.
- W2156020419 hasOpenAccess W2156020419 @default.
- W2156020419 hasPrimaryLocation W21560204191 @default.
- W2156020419 hasRelatedWork W2007980458 @default.
- W2156020419 hasRelatedWork W2032618026 @default.
- W2156020419 hasRelatedWork W2341434149 @default.
- W2156020419 hasRelatedWork W2521199450 @default.
- W2156020419 hasRelatedWork W2577300078 @default.
- W2156020419 hasRelatedWork W2947795167 @default.
- W2156020419 hasRelatedWork W2963062456 @default.
- W2156020419 hasRelatedWork W3019069663 @default.
- W2156020419 hasRelatedWork W4200378785 @default.
- W2156020419 hasRelatedWork W4300628354 @default.
- W2156020419 hasVolume "66" @default.
- W2156020419 isParatext "false" @default.
- W2156020419 isRetracted "false" @default.
- W2156020419 magId "2156020419" @default.
- W2156020419 workType "article" @default.